LOCUS       sequence-204962-       13547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with puro selection for expression of S. aureus 
            dCas9-KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, 
            Gersbach CA
  TITLE     RNA-guided transcriptional silencing in vivo with S. aureus 
            CRISPR-Cas9 repressors.
  JOURNAL   Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
  PUBMED    29700298
REFERENCE   2  (bases 1 to 13547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-26"; volume: "9(1):1674. doi"; pages: " 
            10.1038/s41467-018-04048-4"
COMMENT     Sequence Label: pLV hUbC-dSaCas9-KRAB-T2A-PuroR
FEATURES             Location/Qualifiers
     source          1..13547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        112..491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     646..666
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             709..889
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    936..1061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1554..1787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1972..2016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2314..2431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2496..3707
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3544..3563
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3745..3771
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3778..3798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6979..7026
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7060..7254
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             7321..7341
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7348..7401
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7402..8001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7402..7421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7898..7918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(8055..8088)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8113..8701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8166..8186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8584..8595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8704..8720)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8705..8721)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9226..9406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9432..9449)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9438..9662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9708..10136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9795..9814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10005..10026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10145..10165)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10150..10479
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10330..10465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10392..10411
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10527..10574
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10593..10967
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11097..11218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11134..11153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11188..11207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11267..11283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11267..11283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11280..11302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11291..11307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11315..11321,11322..11339,11340..11345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11360..11381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11498..11515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11669..12257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11749..11768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12428..13219,13220..13288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13051..13070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13289..13393)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13468..13487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
       61 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      121 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      181 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      241 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      301 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      361 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      421 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      481 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      541 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      601 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      661 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      901 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      961 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1021 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1081 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1141 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1201 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1261 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1321 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1381 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1441 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1501 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1561 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1621 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1681 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1741 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1801 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1861 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1921 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1981 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2041 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2101 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2161 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2221 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2281 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2341 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2401 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2461 agtttggtta attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc
     2521 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
     2581 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
     2641 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
     2701 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
     2761 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     2821 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     2881 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     2941 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     3001 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     3061 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     3121 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     3181 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     3241 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     3301 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     3361 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     3421 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     3481 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     3541 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     3601 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     3661 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg
     3721 caggtcgact ctagagccac catgtaccca tacgatgttc cagattacgc tatggcccca
     3781 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccaagcggaa ctacatcctg
     3841 ggcctggcca tcggcatcac cagcgtgggc tacggcatca tcgactacga gacacgggac
     3901 gtgatcgatg ccggcgtgcg gctgttcaaa gaggccaacg tggaaaacaa cgagggtagg
     3961 cggagcaaga gaggcgccag aaggctgaag cggcggaggc ggcatagaat ccagagagtg
     4021 aagaagctgc tgttcgacta caacctgctg accgaccaca gcgagctgag cggcatcaac
     4081 ccctacgagg ccagagtgaa gggcctgagc cagaagctga gcgaggaaga gttctctgcc
     4141 gccctgctgc acctggccaa gagaagaggc gtgcacaacg tgaacgaggt ggaagaggac
     4201 accggcaacg agctgtccac caaagagcag atcagccgga acagcaaggc cctggaagag
     4261 aaatacgtgg ccgaactgca gctggaacgg ctgaagaaag acggcgaagt gcggggcagc
     4321 atcaacagat tcaagaccag cgactacgtg aaagaagcca aacagctgct gaaggtgcag
     4381 aaggcctacc accagctgga ccagagcttc atcgacacct acatcgacct gctggaaacc
     4441 cggcggacct actatgaggg acctggcgag ggcagcccct tcggctggaa ggacatcaaa
     4501 gaatggtacg agatgctgat gggccactgc acctacttcc ccgaggaact gcggagcgtg
     4561 aagtacgcct acaacgccga cctgtacaac gccctgaacg acctgaacaa tctcgtgatc
     4621 accagggacg agaacgagaa gctggaatat tacgagaagt tccagatcat cgagaacgtg
     4681 ttcaagcaga agaagaagcc caccctgaag cagatcgcca aagaaatcct cgtgaacgaa
     4741 gaggatatta agggctacag agtgaccagc accggcaagc ccgagttcac caacctgaag
     4801 gtgtaccacg acatcaagga cattaccgcc cggaaagaga ttattgagaa cgccgagctg
     4861 ctggatcaga ttgccaagat cctgaccatc taccagagca gcgaggacat ccaggaagaa
     4921 ctgaccaatc tgaactccga gctgacccag gaagagatcg agcagatctc taatctgaag
     4981 ggctataccg gcacccacaa cctgagcctg aaggccatca acctgatcct ggacgagctg
     5041 tggcacacca acgacaacca gatcgctatc ttcaaccggc tgaagctggt gcccaagaag
     5101 gtggacctgt cccagcagaa agagatcccc accaccctgg tggacgactt catcctgagc
     5161 cccgtcgtga agagaagctt catccagagc atcaaagtga tcaacgccat catcaagaag
     5221 tacggcctgc ccaacgacat cattatcgag ctggcccgcg agaagaactc caaggacgcc
     5281 cagaaaatga tcaacgagat gcagaagcgg aaccggcaga ccaacgagcg gatcgaggaa
     5341 atcatccgga ccaccggcaa agagaacgcc aagtacctga tcgagaagat caagctgcac
     5401 gacatgcagg aaggcaagtg cctgtacagc ctggaagcca tccctctgga agatctgctg
     5461 aacaacccct tcaactatga ggtggaccac atcatcccca gaagcgtgtc cttcgacaac
     5521 agcttcaaca acaaggtgct cgtgaagcag gaagaagcca gcaagaaggg caaccggacc
     5581 ccattccagt acctgagcag cagcgacagc aagatcagct acgaaacctt caagaagcac
     5641 atcctgaatc tggccaaggg caagggcaga atcagcaaga ccaagaaaga gtatctgctg
     5701 gaagaacggg acatcaacag gttctccgtg cagaaagact tcatcaaccg gaacctggtg
     5761 gataccagat acgccaccag aggcctgatg aacctgctgc ggagctactt cagagtgaac
     5821 aacctggacg tgaaagtgaa gtccatcaat ggcggcttca ccagctttct gcggcggaag
     5881 tggaagttta agaaagagcg gaacaagggg tacaagcacc acgccgagga cgccctgatc
     5941 attgccaacg ccgatttcat cttcaaagag tggaagaaac tggacaaggc caaaaaagtg
     6001 atggaaaacc agatgttcga ggaaaagcag gccgagagca tgcccgagat cgaaaccgag
     6061 caggagtaca aagagatctt catcaccccc caccagatca agcacattaa ggacttcaag
     6121 gactacaagt acagccaccg ggtggacaag aagcctaata gagagctgat taacgacacc
     6181 ctgtactcca cccggaagga cgacaagggc aacaccctga tcgtgaacaa tctgaacggc
     6241 ctgtacgaca aggacaatga caagctgaaa aagctgatca acaagagccc cgaaaagctg
     6301 ctgatgtacc accacgaccc ccagacctac cagaaactga agctgattat ggaacagtac
     6361 ggcgacgaga agaatcccct gtacaagtac tacgaggaaa ccgggaacta cctgaccaag
     6421 tactccaaaa aggacaacgg ccccgtgatc aagaagatta agtattacgg caacaaactg
     6481 aacgcccatc tggacatcac cgacgactac cccaacagca gaaacaaggt cgtgaagctg
     6541 tccctgaagc cctacagatt cgacgtgtac ctggacaatg gcgtgtacaa gttcgtgacc
     6601 gtgaagaatc tggatgtgat caaaaaagaa aactactacg aagtgaatag caagtgctat
     6661 gaggaagcta agaagctgaa gaagatcagc aaccaggccg agtttatcgc ctccttctac
     6721 aacaacgatc tgatcaagat caacggcgag ctgtatagag tgatcggcgt gaacaacgac
     6781 ctgctgaacc ggatcgaagt gaacatgatc gacatcacct accgcgagta cctggaaaac
     6841 atgaacgaca agaggccccc caggatcatt aagacaatcg cctccaagac ccagagcatt
     6901 aagaagtaca gcacagacat tctgggcaac ctgtatgaag tgaaatctaa gaagcaccct
     6961 cagatcatca aaaagggcaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
     7021 aaaaagggat ccgatgctaa gtcactgact gcctggtccc ggacactggt gaccttcaag
     7081 gatgtgtttg tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc
     7141 ctgtacagaa atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt
     7201 actaagccag atgtgatcct ccggttggag aagggagaag agccctggct ggtggagaga
     7261 gaaattcacc aagagaccca tcctgattca gagactgcat ttgaaatcaa atcatcagtt
     7321 ccgaaaaaga aacgcaaagt tgctagcgag ggcagaggaa gtcttctaac atgcggtgac
     7381 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     7441 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7501 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7561 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7621 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7681 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7741 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt gtcgcccgac
     7801 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7861 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7921 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7981 acccgcaagc ccggtgcctg accagcacac tggcggccgt tactagcttc tgcagcacga
     8041 ccggttgata atagataact tcgtatagca tacattatac gaagttatga attcgatatc
     8101 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8161 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8221 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8281 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8341 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8401 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8461 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8521 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8581 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8641 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8701 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     8761 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8821 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8881 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8941 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     9001 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9061 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     9121 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     9181 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     9241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     9421 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9481 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9541 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9601 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     9661 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     9721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9841 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9901 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9961 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10021 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10081 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    10141 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10201 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10261 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10381 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    10441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    10501 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    10561 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    10621 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    10681 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    10741 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    10801 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    10861 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    10921 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    10981 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11041 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11101 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11161 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11221 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11281 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11341 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11401 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11461 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    11581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    11641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    11701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    11761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    11821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    11881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    11941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    12601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    12661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    12721 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    12781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    12841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    12901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13021 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    13441 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    13501 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcga
//
