LOCUS       sequence-204997-        6460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA-mCherry fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TCB
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     420..439
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    492..525
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    588..621
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(628..1335)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(906..925)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1175..1193
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1770..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1786..1819)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1890..1923)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1947..2535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2000..2020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2418..2429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2559..3035
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2758..2777)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3081..3221
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3081..3210
                     /label=AAV2 ITR
     rep_origin      3296..3751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3383..3402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3593..3614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3768..3787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3887..3909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3947..3965)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4033..4137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4138..4206,4207..4998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4356..4375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5169..5757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5658..5677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5819..5948
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        6038..6341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
      421 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgattt gataccgcgg
      481 gcccggccgg gataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga
      541 ccaatgcttc accatcgacc cgaattgcca agcatcacca tcgacacata acttcgtata
      601 atgtatgcta tacgaagtta tgtcgactta cttgtacagc tcgtccatgc cgccggtgga
      661 gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      721 gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt
      781 gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt
      841 gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc
      901 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac
      961 cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc
     1021 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta
     1081 gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga
     1141 caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg
     1201 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
     1261 ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc
     1321 ctcgcccttg ctcaccccac cccccttgga tgcgctttca agactgaaga tgtcagacct
     1381 gctttttgct ttggacttcc cccatgcagc gataccaccc acagctacca ggcagcacag
     1441 gagcactgca gtgatcagca tccacatgcg gccgtgattc ctggctggca gagcacgtcc
     1501 aggagcaggg gcagtgggag cctctgtgcc gttgtccgtg ggcaccgcgg ggatcgcgct
     1561 ggtcccgcag ccccactcgt cccgcccgtc gtcgcagtcg gggtgtccgt cgcacagcca
     1621 gtcctgcggg taacactccc cgtgggcacc gggcggctcc gagcagcgga actgaccggg
     1681 ggggcaacgg gacaaagaac cgttaccgga cccgttaccg gtcacgttac cgggcagcag
     1741 cagcagcagc agcgcgggca gcagccgcgc catggtggcg cgcctataac ttcgtatagg
     1801 atactttata cgaagttatc attgggattc ttcctatttt gatccaagca tcaccatcga
     1861 ccctctagtc cagatctcac catcgaccca taacttcgta tagcatacat tatacgaagt
     1921 tataagctta tatgcggccg atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     1981 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2041 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2101 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2161 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2221 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2281 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2341 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2401 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2461 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2521 gggccgcctc cccgcatcga taccgtagat cgatctacgg gtggcatccc tgtgacccct
     2581 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     2641 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     2701 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     2761 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     2821 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     2881 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     2941 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3001 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3061 gaccgaacta gtgcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3121 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3181 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3241 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     3301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3361 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3481 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     3541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3601 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     3661 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3721 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     3781 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3841 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3901 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3961 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4021 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4081 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4141 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4201 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4261 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4321 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4381 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4441 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4501 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4561 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4621 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4681 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4741 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4801 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4861 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4921 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4981 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5041 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc
     5821 tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg
     5881 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     5941 gggttcctgc ggccgcacgc gtaattcccg gggaatctag ttattaatag taatcaatta
     6001 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     6061 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     6121 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     6181 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     6241 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     6301 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     6361 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     6421 tttaattatt ttgtgcagcg atgggggcgg gggggggggg
//
