LOCUS       sequence-205114-        7651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI 
            CIDs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..7651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..38)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22..38)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(35..57)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(70..76,77..94,95..100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      221..355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    375..509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(412..431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     466..485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(577..594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(748..1336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(828..847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1507..2298,2299..2367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2130..2149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2368..2472)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2503..2882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2884..3159
                     /label=chicken beta-actin promoter
     intron          3160..4175
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4099..4121
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4183..4202
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4322..4339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6674..6697
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6698..6718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6728..6952
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7192..7211)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7257..7312
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7311..7330)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
       61 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      181 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      361 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      481 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
      541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1381 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1441 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1501 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1561 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1621 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1681 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1741 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1801 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1861 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1921 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1981 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2041 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2101 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2161 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2221 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2281 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2341 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2401 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2461 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     2521 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2581 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2641 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2701 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2761 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2821 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2881 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     2941 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     3001 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt
     3061 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg
     3181 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     3241 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     3301 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
     3361 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     3421 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     3481 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     3541 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     3601 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     3661 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3721 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3781 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3841 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3901 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     3961 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     4021 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     4081 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     4141 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     4201 tgctgtctca tcattttggc aaagaattga ttaattcgag cgaaggcgcg cccattactc
     4261 gcatccattc tcaggctgtc tcgtctcgtc tcgcacgcgt gccgccacca tgaagcggga
     4321 ccaccaccat caccatcatc aggacaagaa aaccatgatg atgaacgaag aggacgacgg
     4381 caacggcatg gacgagctgc tggctgtgct gggctacaaa gtgcggagca gcgagatggc
     4441 cgacgtggcc cagaaactgg aacagctgga agtgatgatg agcaacgtgc aggaagatga
     4501 cctgtcccag ctggccaccg agacagtgca ctacaacccc gccgagctgt acacctggct
     4561 ggactccatg ctgaccgacc tgaacgggcc ggccggcggc ggtagcggcg gtggctctgg
     4621 cggtcctgca gggggcgaga agatcgccag ctgtgccgcc gagctgacct acctgtgctg
     4681 gatgatcacc cacaacggca ccgccatcaa gagggccacc ttcatgagct acaacaccat
     4741 catcagcaac agcctgagct tcgacatcgt gaacaagagc ctgcagttca agtacaagac
     4801 ccagaaggcc accatcctgg aggccagcct gaagaagctg atccccgcct gggagttcac
     4861 catcatccct tacaacggcc agaagcacca gagcgacatc accgacatcg tgtccagcct
     4921 gcagctgcag ttcgagagca gcgaggaggc cgacaagggc aacagccaca gcaagaagat
     4981 gctgaaggcc ctgctgtccg agggcgagag catctgggag atcaccgaga agatcctgaa
     5041 cagcttcgag tacaccagca ggttcaccaa gaccaagacc ctgtaccagt tcctgttcct
     5101 ggccacattc atcaactgcg gcaggttcag cgacatcaag aacgtggacc ccaagagctt
     5161 caagctggtg cagaacaagt acctgggcgt gatcattcag tgcctggtga ccgagaccaa
     5221 gacaagcgtg tccaggcaca tctacttttt cagcgccaga ggcaggatcg accccctggt
     5281 gtacctggac gagttcctga ggaacagcga gcccgtgctg aagagagtga acaggaccgg
     5341 caacagcagc agcaacaagc aggagtacca gctgctgaag gacaacctgg tgcgcagcta
     5401 caacaaggcc ctgaagaaga acgcccccta ccccatcttc gctatcaaga acggccctaa
     5461 gagccacatc ggcaggcacc tgatgaccag ctttctgagc atgaagggcc tgaccgagct
     5521 gacaaacgtg gtgggcaact ggagcgacaa gagggcctcc gccgtggcca ggaccaccta
     5581 cacccaccag atcaccgcca tccccgacca ctacttcgcc ctggtgtcca ggtactacgc
     5641 ctacgacccc atcagcaagg agatgatcgc cctgaaggac gagaccaacc ccatcgagga
     5701 gtggcagcac atcgagcagc tgaagggcag cgccgagggc tccggagggt ctggctccgg
     5761 atcaagtggt ggcagcggta cccctttgta tggttttact tcgatttgtg gaagaagacc
     5821 tgagatggaa gatgctgttt cgactatacc aagattcctt caatcttcct ctggttcgat
     5881 gttagatggt cggtttgatc ctcaatccgc cgctcatttc ttcggtgttt acgacggcca
     5941 tggcggttct caggtagcga actattgtag agagaggatg catttggctt tggcggagga
     6001 gatagctaag gagaaaccga tgctctgcga tggtgatacg tggctggaga agtggaagaa
     6061 agctcttttc aactcgttcc tgagagttga ctcggagatt gggtcagttg cgccggagac
     6121 ggttgggtca acgtcggtgg ttgccgttgt tttcccgtct cacatcttcg tcgctaactg
     6181 cggtgactct agagccgttc tttgccgcgg caaaactgca cttccattat ccgttgacca
     6241 taaaccggat agagaagatg aagctgcgag gattgaagcc gcaggaggga aagtgattca
     6301 gtggaatgga gctcgtgttt tcggtgttct cgccatgtcg agatccattg gcgatagata
     6361 cttgaaacca tccatcattc ctgatccgga agtgacggct gtgaagagag taaaagaaga
     6421 tgattgtctg attttggcga gtgacggggt ttgggatgta atgacggatg aagaagcgtg
     6481 tgagatggca aggaagcgga ttctcttgtg gcacaagaaa aacgcggtgg ctggggatgc
     6541 atcgttgctc gcggatgagc ggagaaagga agggaaagat cctgcggcga tgtccgcggc
     6601 tgagtatttg tcaaagctgg cgatacagag aggaagcaaa gacaacataa gtgtggtggt
     6661 ggttgatttg aaggattaca aggacgatga cgataagccc aagaaaaagc ggaaggtgtg
     6721 agaattcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6781 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6841 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6901 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6961 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cgtgtaccgc
     7021 tgggagttcg tagacggaaa caaacgcaga atccaagcgc ggccgcccag gatacataga
     7081 ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg ctgcctatca
     7141 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     7201 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     7261 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     7321 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     7381 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     7441 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     7501 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     7561 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     7621 tcttctctta tggagatccc tcgacctgca g
//
