LOCUS       sequence-205177-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A 
            mutation in human) in retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula 
            L, Hehnly H
  TITLE     Gravin regulates centrosome function through PLK1.
  JOURNAL   Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524.
  PUBMED    29282278
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 
            10.1091/mbc.E17-08-0524."
COMMENT     Sequence Label: pBABE-SSeCKs T751A
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             40..509
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    574..773
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             974..1390
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1320..1342
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1358..1374
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6516..6536)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6521..6850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6701..6836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6860..7459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6860..6879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7356..7376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7680..8149
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8279..8296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8450..9038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8530..8549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9198..9989,9990..10058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9821..9840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10059..10154,1..9))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttccgcgc acatttcccc gaaaagtgcc acctgcagcc tgaatatggg ccaaacagga
       61 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
      121 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      181 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
      241 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
      301 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
      361 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
      421 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
      481 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
      541 tgcccaggga ccaccgaccc accaccggga ggcaagctgg ccagcaactt atctgtgtct
      601 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
      661 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
      721 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
      781 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
      841 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
      901 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
      961 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1021 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1081 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1141 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1201 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1261 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1321 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1381 tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga atgaattcaa tgggtgcagg
     1441 cagttccacc gagcagcgga gccccgagca gccggcggag agcgacacgc cgagcgagct
     1501 ggagctcagt ggccatgggc ccgcagcgga agcgtcggga gcagctggag atcccgctga
     1561 cgcggacccc gccaccaagc tcccacagaa gaatggtcag ctgtctgccg tcaatggtgt
     1621 agctgaacaa gaagatgtcc acgtccaaga ggaaagccag gatgggcaag aggaagaagt
     1681 cactgttgaa gatgttggac agagagagtc agaagatgtg aaagaaaaag accgagctaa
     1741 agaaatggca gccagttcca cagttgttga agatatcaca aaggacgagc aggaggaaac
     1801 accggaaata atcgaacaga tccctgcttc agagagcaat gtggaagaaa tggcgcaggc
     1861 tgctgagtcc caagctaatg acgtcggctt caagaaggta tttaaatttg ttggttttaa
     1921 attcacggtg aagaaggata aaaacgaaaa gtcagatacc gtccagctac tcactgtcaa
     1981 gaaggatgaa ggcgaagggg cagaagcctc cgtcggagca ggagaccacc aagagcccgg
     2041 agtggagacc gtcggcgaat cagcatccaa agaaagtgag ctgaagcaat ccacagagaa
     2101 gcaagaaggc accctgaagc aagcacagag cagcacagaa attccccttc aagccgaatc
     2161 tggtcaaggg accgaggaag aagcagccaa agatggagaa gaaaaccgag agaaagaacc
     2221 taccaagccc ctagaatctc cgaccagccc tgtcagcaat gagacaacat cttccttcaa
     2281 gaaattcttc actcacggct gggccggctg gcgcaagaag accagcttca agaaaccaaa
     2341 ggaagatgat ctggaaactt ccgagaagag aaaggagcaa gaggctgaaa aagtagacga
     2401 ggaagaaggg gaaaagacag agccagcccc agccgaggag caggagcctg cagaaggcac
     2461 agaccaggcc aggttgtcag ccgactatga gaaggtggag ttgcctttgg aagaccaggt
     2521 cggtgacctg gaggcattgt cggagaagtg tgctcctttg gcaacggaag tgtttgatga
     2581 gaagacggaa gcccaccaag aagttgttgc agaggtccac gtgagcaccg tggagaagat
     2641 gacgaaaggg caaggaggag cagaggtgga aggggatgtg gtggtggaag gatcgggaga
     2701 atccttgccc cctgagaaac tggctgagac ccaggaggtc ccccaggaag ctgagcctgt
     2761 ggaggagctg atgaagacca aagaagtatg cgtctctggg ggtgaccata ctcagctgac
     2821 agatctaagt cctgaagaga agatgctacc caaacacccc gaaggcattg tcagtgaggt
     2881 ggagatgctg tcctctcagg agagaatcaa ggtacaggga agtcccctga agaagctctt
     2941 cagcagttcg ggcttaaaga agctctccgg gaagaagcag aaggggaaga gaggaggagg
     3001 cgggggagat gaagagccag gagaatacca acacattcaa accgagtccc cagagagtgc
     3061 tgacgagcag aagggagaga gctctgcctc ttcccctgaa gagcccgagg agatcgcgtg
     3121 tctggagaag gggccatcgg aagcacccca ggaagcggaa gctgaggaag gagcgacttc
     3181 cgacggagag aagaaaaggg aagggatcac cccctgggca tccttcaaaa agatggtgac
     3241 acccaagaaa cgggtccgaa gaccttctga gagcgacaag gaagaagagc tggataaggt
     3301 caagagtgcc accttgtcct ccacggagag cacggcgtct ggaatgcagg atgaggtcag
     3361 agcggttggc gaggagcaaa ggtcagagga gccaaagcgc agggtggata cttcagtgtc
     3421 ttgggaggcg ttgatttgtg tcggatcgtc caagaagaga gcgaggaagg catcctcttc
     3481 agatgatgaa ggagggccaa gaacactggg aggggatggc cacagagcgg aggaggctag
     3541 caaagacaaa gaagcagatg ctcttcctgc cagcacccag gaacaagacc aagcgcacgg
     3601 aagttcctca cccgagccag ctggaagccc ttctgaaggg gagggcgtct ccacctggga
     3661 gtcatttaag agattagtcg caccacgaaa aaaatccaag tcaaaactgg aagagagagc
     3721 cgaagactcc ggtgcagagc agttggcctc cgagatcgaa ccaagtagag aggaatcttg
     3781 ggtttccatt aagaaattta ttcctggacg gcggaagaaa agggcagatg ggaagcaaga
     3841 acaggccgcc gttgaagact cggggccagg agagatcaat gaggacgacc ccgacgtccc
     3901 agctgttgtg cctctgtctg agtacgatgc ggtagagaga gagaagctgg aagcgcagcg
     3961 agctcaggag aacgtggagc tgccccagct gaagggggct gtgtatgtgt ctgaggagct
     4021 tagtaagact ctggttcaca ccgtgagtgt cgcggtcatt gatgggacca gggcagtcac
     4081 cagtgccgaa gagcggtccc cttcgtggat atctgcttcc atgacagaac ctcttgagca
     4141 cgcagaggga gtggccacac cgcctgttgg agaggtcact gaaaaagaca tcactgcaga
     4201 agcaactcct gcactcgccc agactttacc agggggcaaa gatgcccatg acgacatagt
     4261 caccagtgaa gtggatttta cctcagaagc agtgacagcc gcagaaacca cagaggcgct
     4321 ccgcgctgaa gaacttaccg aagcatcagg ggcagaagag accacagaca tggtgtctgc
     4381 agtttcccag ctgtccgact ccccggacac cacagaggaa gccaccccag ttcaggaggt
     4441 agagggtggc atgctagata cggaagaaca ggagcgccag acgcaggccg tcctccaagc
     4501 cgttgcagac aaagtgaaag aggactccca ggtgcctgca acccagactc tgcagagagc
     4561 agggccgaaa gcactggaga aggtggagga ggtagaggag gactccgagg tgctggctac
     4621 cgagaaagag aaggatgttg tgccggaagg acccgtgcag gaagctgaaa ctgagcatct
     4681 tgcacagggc tccgagactg tacaggctac cccagagagc cttgaagttc ctgaagtcac
     4741 agaggatgta gaccgtgcca ccacatgcca ggttatcaag caccagcagc tgatggaaca
     4801 ggctgtggcc cctgagtcat ctgaaacctt gacagacagt gagacaaatg gaagtactcc
     4861 cctcgcagat tcagacactc caaacgggac acagcaagac gagaccgttg acagccagga
     4921 cagtaatgcc attgccgccg tcaagcagtc acaggtcact gaagaggagg cagctgctgc
     4981 tcagacggag gggccttcaa caccatctag ttttccagcc caggaagaac acagggaaaa
     5041 accaggaagg gatgttctag aacccacaca agcgctggct gccggggcag tgcctattct
     5101 ggcaaaggct gaggtgggtc aagagggtga ggctggccag tttgatggag aaaaagtcaa
     5161 agacggacag tgtgttaaag aactggaggt gcctgtgcac actggaccca acagtcaaaa
     5221 gactgctgac ttgacacgtg acagtgaagt aatggaagtg gccagatgtc aggaaactga
     5281 gagtaatgaa gaacagagta ttagcccgga gaaaagagag atgggaaccg acgttgaaaa
     5341 ggaggaaaca gagaccaaga cagagcaagc cagtgaagaa catgagcagg aaacagctgc
     5401 tcctgagcat gaaggaaccc accctaagcc agtcctgaca gctgacatgc ctcactcaga
     5461 gaggggaaag gcactgggca gccttgaagg aagcccttct ctcccagacc aagacaaagc
     5521 agattgcata gaggttcaag ttcaaagctc agacacacca gtcactcaaa caaccgaagc
     5581 tgtgaaaaag gtcgaagaaa ctgtggcaac ttcagagatg gatgaaagtt tggagtgtgc
     5641 aggtgcgcaa tcattaccag ctgagaagct ctccgaaacc ggtggctacg ggactcttca
     5701 gcatggagag gacaccgtgc cccaggggcc tgagtctcag gcagagtcca tcccaataat
     5761 agtaactcct gctcctgaaa gcatcctaca ttctgacctt caaagagaag tgagcgcatc
     5821 ccagaaacag agatcagatg aagataacaa gccagatgct ggtcctgatg ctgccagcaa
     5881 ggagagtgca gcaagagaga aaatcctcag ggctgaacct gagatcttgg aacttgagag
     5941 taagagcaat aagattgtcc agagtgtcat ccagacagcc gtcgaccagt ttgcacgtac
     6001 agaaacagcc cccgaaaccc acgcttctga tttacagaat caggttcctg tgatgcaggc
     6061 tgacagccag ggagcacaac agatgctgga caaagatgaa agcgaccttc aagtctcccc
     6121 ccaagatgga acactcagtg ccgtagccca ggaaggactt gcggtttctg atagttctga
     6181 aggcatgagc aaggcttcag aaatgatcac cacgcttgca gttgaaagtg ccagtgtcaa
     6241 agaaagtgtt gagaagctgc ctcttcagtg caaagatgaa aaggaacatg ctgctgacgg
     6301 cccccagcac caaagcttag ccaaggcaga ggcggatgcc tctggaaatc taaccaaaga
     6361 gtccccagac accaacggac caaagctaac cgaggaagga gatgccctga aagaagaaat
     6421 gaacaaggcc cagacagaag aggacgacct acaggagcca aagggagacc tgacagaatc
     6481 ctaactcgag tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
     6541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6601 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6661 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6841 cttttgcaaa aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     7021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     7081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     7141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7441 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     7501 acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     7561 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     7621 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     7681 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     7741 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     7801 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     7861 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     7921 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     7981 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     8041 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     8101 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     8161 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     8221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8281 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8341 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8881 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9121 caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag
     9181 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9241 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9301 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9361 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9421 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9481 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9541 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9601 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9661 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9721 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9781 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9841 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9901 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9961 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10021 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10081 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10141 aataaacaaa tagg
//
