LOCUS       sequence-205407-       11318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL3
FEATURES             Location/Qualifiers
     source          1..11318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     476..495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     729..746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    863..884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(899..904,905..922,923..929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    937..953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     942..964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     961..977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     961..977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     996..1016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        998..1016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1044..1270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1271..1451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1498..1623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2116..2349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2534..2578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2845..2961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2995..3019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3150..4328
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3381..4319
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4276..4296
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4345..4363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4408..4410,4411..4413,4414..5124)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4408..4410,4411..4413,4414..5124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4453..4474)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4714..4733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5061..5082
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6726..6750)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6781..7369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6834..6854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7252..7263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7420..7919
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7455..7478)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7852..7871
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7933..8736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7996..8015)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8606..8625
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8880..9113
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9185..9306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9222..9241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9276..9295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9346..9481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9502..9520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9530..9547)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9530..9546)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9539..9561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9688..10143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9775..9794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9985..10006
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10169..10273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10274..10342,10343..11134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10492..10511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11305..11318,1..575)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
       61 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      961 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     1021 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     1081 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     1141 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     1201 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     1261 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     1321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     1381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1441 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1501 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1561 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1621 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1681 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1741 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1801 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1861 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1921 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1981 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     2041 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     2101 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     2161 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     2221 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     2281 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     2341 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     2401 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2461 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2521 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2581 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2641 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2701 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2761 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2821 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     2881 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2941 aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcaacaagt
     3001 ttgtacaaaa aagcaggctc caccatggga accgacattg attattgact aggcttttgc
     3061 aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     3121 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     3181 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     3241 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     3301 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     3361 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3421 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     3481 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     3541 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3601 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3661 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3721 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3781 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3841 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3901 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3961 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     4021 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     4081 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     4141 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     4201 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     4261 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     4321 tgtcgtgagg aattagcttg gtactaatac gactcactat agggagaccc aagctggcta
     4381 ggtaagcttg gtaccgagct cggatccatg gtgagcaagg gcgaggagct gttcaccggg
     4441 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4501 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4561 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4621 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4681 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4741 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4801 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4861 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4921 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4981 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5041 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5101 ctcggcatgg acgagctgta caagggtgga tctggaggtt ctggtggaat ggagagcagc
     5161 aagcctggtc cagtgcaggt tgttttggtt cagaaagatc aacattcctt tgagctagat
     5221 gagaaagcct tggccagcat cctcttgcag gaccacatcc gagatcttga tgtggtggtg
     5281 gtttcagtgg ctggtgcctt ccgaaagggc aagtccttca ttctggattt tatgctacga
     5341 tacttatatt ctcagaagga aagtggccat tcaaattggt tgggtgaccc agaagaaccg
     5401 ttaacaggat tttcctggag agggggatct gatccagaaa ccactgggat tcaaatctgg
     5461 agtgaagttt tcactgtgga gaagccaggt gggaagaagg ttgcagttgt tctgatggat
     5521 acccaggggg catttgacag ccagtcaact gtgaaagact gtgctaccat ctttgctcta
     5581 agcactatga ctagttctgt tcagatttat aatttatctc agaacattca agaagatgat
     5641 cttcaacagc tgcagctctt cacagaatac ggtcgtctgg caatggatga aattttccaa
     5701 aagcctttcc agacactgat gtttttggtt agagattgga gtttccctta tgaatatagc
     5761 tatggactcc aaggaggaat ggcatttttg gataagcgtt tacaggtgaa ggaacatcaa
     5821 catgaagaaa ttcagaatgt tcgaaatcac attcactcat gtttctccga tgtcacctgc
     5881 tttctcttac cacatccagg actccaggtg gccacaagcc ctgactttga tgggaaatta
     5941 aaagatattg ctggtgaatt caaagagcag ttacaggcac tgataccgta tgtattaaac
     6001 ccatctaagt taatggaaaa ggagatcaat ggctcaaagg tcacctgtcg gggactactg
     6061 gagtatttta aggcatatat taaaatttat caaggagaag atctgcctca ccccaagtcc
     6121 atgcttcagg ccactgctga agccaacaac ttagcagctg cagcctctgc caaggacatt
     6181 tattataaca acatggaaga ggtttgtggg ggagagaaac cttatttgtc tccagacatt
     6241 ctagaggaga agcactgtga attcaaacaa cttgctctgg accattttaa gaagaccaag
     6301 aagatgggtg ggaaggattt cagctttcgt taccagcagg agctggagga ggaaatcaag
     6361 gaattatatg agaacttctg caagcacaat ggtagcaaga acgtcttcag caccttccga
     6421 acccctgcag tgctgttcac gggcattgta gctttgtaca tagcctcagg cctcactggc
     6481 ttcataggtc ttgaggttgt agcccagttg ttcaactgta tggttggact actgttaata
     6541 gcactcctca cctggggcta catcaggtat tctggtcaat atcgtgagct gggcggagct
     6601 attgattttg gtgccgcata tgtgttggag caggcttctt ctcatatcgg taattccact
     6661 caggccactg tgagggatgc agttgttgga agaccatcca tggataaaaa agctcaatag
     6721 tctagaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgac
     6781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6841 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6901 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6961 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7021 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7081 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7141 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7201 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7261 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7321 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggacctgca
     7381 ggattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag gcgcttttcc
     7441 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     7501 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     7561 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     7621 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     7681 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     7741 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     7801 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     7861 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     7921 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     7981 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8041 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8101 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     8161 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8221 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8281 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8341 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8401 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8461 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8521 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8581 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8641 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8701 atcgccttct atcgccttct tgacgagttc ttctgaacgc gtcgcccgcc ccacgacccg
     8761 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     8821 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8881 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8941 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9001 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9061 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9121 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9181 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9241 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9301 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9361 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9421 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9481 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     9541 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     9601 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     9661 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     9721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9841 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9901 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9961 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10021 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10081 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    10141 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    10201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    10261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    10321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    10381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    10441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    10501 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    10561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    10621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    10681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    10741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    10801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    10861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    11041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    11101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    11161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    11221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11281 cgtagaaaag atcaaaggat cttcttgaga tccttttt
//
