LOCUS       sequence-205408-       11030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL3-deltaTM
FEATURES             Location/Qualifiers
     source          1..11030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     476..495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     729..746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    863..884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(899..904,905..922,923..929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    937..953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     942..964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     961..977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     961..977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     996..1016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        998..1016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1044..1270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1271..1451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1498..1623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2116..2349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2534..2578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2845..2961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2995..3019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3150..4328
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3381..4319
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4276..4296
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4345..4363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4408..4410,4411..4413,4414..5124)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4408..4410,4411..4413,4414..5124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4453..4474)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4714..4733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5061..5082
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6438..6462)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6493..7081
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6546..6566)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6964..6975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7132..7631
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7167..7190)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7564..7583
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7645..8448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7708..7727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8318..8337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8592..8825
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8897..9018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8934..8953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8988..9007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9058..9193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9214..9232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9242..9259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9242..9258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9251..9273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9400..9855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9487..9506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9697..9718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9881..9985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9986..10054,10055..10846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10204..10223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11017..11030,1..575)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
       61 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      961 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     1021 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     1081 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     1141 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     1201 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     1261 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     1321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     1381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1441 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1501 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1561 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1621 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1681 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1741 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1801 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1861 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1921 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1981 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     2041 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     2101 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     2161 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     2221 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     2281 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     2341 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     2401 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2461 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2521 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2581 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2641 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2701 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2761 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2821 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     2881 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2941 aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcaacaagt
     3001 ttgtacaaaa aagcaggctc caccatggga accgacattg attattgact aggcttttgc
     3061 aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     3121 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     3181 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     3241 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     3301 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     3361 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3421 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     3481 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     3541 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     3601 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     3661 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     3721 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3781 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3841 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3901 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3961 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     4021 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     4081 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     4141 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     4201 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     4261 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     4321 tgtcgtgagg aattagcttg gtactaatac gactcactat agggagaccc aagctggcta
     4381 ggtaagcttg gtaccgagct cggatccatg gtgagcaagg gcgaggagct gttcaccggg
     4441 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4501 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4561 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4621 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4681 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4741 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4801 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4861 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4921 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4981 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5041 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5101 ctcggcatgg acgagctgta caagggtgga tctggaggtt ctggtggaat ggagagcagc
     5161 aagcctggtc cagtgcaggt tgttttggtt cagaaagatc aacattcctt tgagctagat
     5221 gagaaagcct tggccagcat cctcttgcag gaccacatcc gagatcttga tgtggtggtg
     5281 gtttcagtgg ctggtgcctt ccgaaagggc aagtccttca ttctggattt tatgctacga
     5341 tacttatatt ctcagaagga aagtggccat tcaaattggt tgggtgaccc agaagaaccg
     5401 ttaacaggat tttcctggag agggggatct gatccagaaa ccactgggat tcaaatctgg
     5461 agtgaagttt tcactgtgga gaagccaggt gggaagaagg ttgcagttgt tctgatggat
     5521 acccaggggg catttgacag ccagtcaact gtgaaagact gtgctaccat ctttgctcta
     5581 agcactatga ctagttctgt tcagatttat aatttatctc agaacattca agaagatgat
     5641 cttcaacagc tgcagctctt cacagaatac ggtcgtctgg caatggatga aattttccaa
     5701 aagcctttcc agacactgat gtttttggtt agagattgga gtttccctta tgaatatagc
     5761 tatggactcc aaggaggaat ggcatttttg gataagcgtt tacaggtgaa ggaacatcaa
     5821 catgaagaaa ttcagaatgt tcgaaatcac attcactcat gtttctccga tgtcacctgc
     5881 tttctcttac cacatccagg actccaggtg gccacaagcc ctgactttga tgggaaatta
     5941 aaagatattg ctggtgaatt caaagagcag ttacaggcac tgataccgta tgtattaaac
     6001 ccatctaagt taatggaaaa ggagatcaat ggctcaaagg tcacctgtcg gggactactg
     6061 gagtatttta aggcatatat taaaatttat caaggagaag atctgcctca ccccaagtcc
     6121 atgcttcagg ccactgctga agccaacaac ttagcagctg cagcctctgc caaggacatt
     6181 tattataaca acatggaaga ggtttgtggg ggagagaaac cttatttgtc tccagacatt
     6241 ctagaggaga agcactgtga attcaaacaa cttgctctgg accattttaa gaagaccaag
     6301 aagatgggtg ggaaggattt cagctttcgt taccagcagg agctggagga ggaaatcaag
     6361 gaattatatg agaacttctg caagcacaat ggtagcaaga acgtcttcag caccttccga
     6421 acccctgcat agtctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     6481 cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6541 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6601 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6661 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6721 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6781 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6841 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6901 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6961 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7021 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7081 cctggacctg caggattctg cagatatccg gactagtgat ctaattctac cgggtagggg
     7141 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     7201 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     7261 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     7321 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     7381 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     7441 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     7501 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     7561 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     7621 cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
     7681 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7741 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7801 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7861 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7921 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7981 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     8041 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     8101 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     8161 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     8221 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8281 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8341 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8401 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac gcgtcgcccg
     8461 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc
     8521 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8581 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     8641 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8701 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8761 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8821 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8881 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9001 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     9061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9181 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     9241 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     9301 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9361 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     9421 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9481 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9541 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9601 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9661 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9721 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9781 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9841 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9901 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9961 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10021 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10081 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10141 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10201 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10261 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10321 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10381 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10441 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10501 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10561 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10621 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10681 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10741 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10801 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10861 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10921 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10981 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
//
