LOCUS       sequence-205464-       13009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-412
FEATURES             Location/Qualifiers
     source          1..13009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     921..940
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(960..1037)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1243..1262
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1346..1365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1365..1389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1443..1460
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5008..5097
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5110..5199
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5679..6332
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             6339..6356
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             8054..8143
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             8084..8173
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(9289..9307)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9303..9350
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9387..9842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9474..9493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9684..9705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9934..10749)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9934..10749)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10657..10676
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10741..10760
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10871..11459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11360..11379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11613..11630
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11645..11784
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11710..11729)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11870..11892
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11886..12077)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(12886..13009,1..959))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
       61 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
      121 gagctgtcct cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      181 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
      241 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
      301 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
      361 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
      421 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa
      481 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
      541 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
      601 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
      661 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
      721 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
      781 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
      841 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
      901 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
      961 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     1021 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     1081 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     1141 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     1201 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     1261 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     1321 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     1381 aacaattccc ctctagaaat aattttgcct taactttaag aaggagatat accatgggca
     1441 gccaccacca ccaccaccac agcggaatgc agatctttgt taagactctc accggaaaga
     1501 caatcaccct agaggtggaa agctccgaca ccatcgacaa cgttaaggcc aagatccagg
     1561 ataaggaggg cattcctccg gatcagcaga ggcttatttt cgccggcaag cagctagagg
     1621 atggccgtac gttggctgat tacaatatcc agaaggaatc caccctccac ttggtcctca
     1681 ggctccgtgg tggttaagct tgcaattaac tttaagaagg agatatacaa tgcttcacaa
     1741 gcgagctagt gaagctaacg ataagaacga taataccatc atcggttccg atttagcatc
     1801 atctaagaaa cgccgtatcg atttcactga atcttcgtct gataagtcat cttcgatttt
     1861 agcttctggt agtagcaggg gttttcacgg cgatagcgtc gttcagcaaa tcgacatggc
     1921 ttttggtaat tcgaaccgtc aggagattga tgaagatctg cacagtcgcc agctcgccgt
     1981 ctatggtcgt gagactatga ggcgtctctt tgcttccaat gttctcatct cggggatgca
     2041 cggtcttggt gccgagattg ccaagaatct tatacttgct ggtgtgaagt ctgtgacctt
     2101 gcatgatgaa agagttgtag agctatggga cttatcaagc aactttgttt tctcggagga
     2161 tgatgttggc aagaatagag cagatgcttc tgttcagaag ttacaggatc ttaacaatgc
     2221 tgtggttgtc tctagcttga ccaaaagctt aaacaaagag gatctttctg gcttccaggt
     2281 tgttgtattc tctgacataa gcatggaaag agcaatcgaa tttgatgact attgtcacag
     2341 ccatcagcct cctatcgctt ttgtcaaggc tgatgtcagg ggtcttttcg gctctgtctt
     2401 ttgtgatttt gggcctgaat ttgcggttct tgatgttgat ggagaggaac cccatacagg
     2461 catcattgcg tctatctcta atgagaacca ggcatttatt tcctgtgtcg acgatgagag
     2521 gcttgagttt gaagatggtg acctagttgt tttctcagaa gttgagggta tgacagaatt
     2581 gaatgatggg aaaccaagga agataaaaag tacacggcca tattcattca cccttgatga
     2641 ggacactact aactatggaa catatgtgaa gggtggtatt gtcactcaag tgaaacagcc
     2701 aaagttactg aatttcaagc cattgaggga agcgcttaag gatccagggg attttctatt
     2761 tagtgatttc tctaagtttg atcggcctcc actccttcac ttagcattcc aggcacttga
     2821 tcattttaaa gctgaagctg gcagatttcc tgttgctggc tcagaagagg acgctcagaa
     2881 gcttatatcg attgccacag ccatcaatac cgggcagggt gatttaaagg tggagaatgt
     2941 tgatcagaag cttctaagac acttttcctt tggagccaag gctgtcctga atcctatggc
     3001 tgcaatgttt ggcggtattg ttggacagga ggttgttaaa gcttgctctg gaaaatttca
     3061 tcctctcttc cagtttttct actttgattc agtggagtca ctcccctctg aacctgtgga
     3121 ctcgagtgac tttgcaccaa gaaacagccg gtatgatgcc caaatatctg tatttggggc
     3181 caagttccag aagaaacttg aagatgctaa agttttcaca gtagggtctg gtgctcttgg
     3241 ctgtgagttc ttgaaaaatt tggctctgat gggggtttct tgtggaagcc aagggaagct
     3301 gacagtgaca gatgatgata tcattgagaa gagtaacctc agtcgtcaat ttctgttccg
     3361 tgactggaac attggacagg ctaaatccac agttgctgct tcggctgctg ctgttataaa
     3421 tcctcgcttc aacattgagg ccctgcaaaa ccgtgttggc gcggagactg agaatgtatt
     3481 tgacgatgcc ttctgggaga acttaactgt tgtcgtcaat gcactggata atgttaatgc
     3541 gaggctctat gttgattcga ggtgcttgta tttccagaag cctctccttg agtctggaac
     3601 tcttggtaca aagtgcaaca cacagtcggt tatcccacat ctaactgaaa attacggtgc
     3661 ctcaagggac ccaccagaga aacaggcccc catgtgtaca gtgcattcct tcccgcataa
     3721 cattgatcac tgtttaactt gggctcgctc tgagtttgag ggtctgctgg agaaaactcc
     3781 cgctgaagtg aatgcatatc tttctagccc agttgagtac actaactcaa tgatgagtgc
     3841 tggtgatgct caggcgaggg acacattgga gaggattgtt gagtgccttg aaaaggagaa
     3901 gtgtgaaacc ttccaagact gcttaacctg ggcacgactg aggtttgagg attactttgt
     3961 gaaccgtgtc aagcaattga tatacacatt tcctgaagat gctgcgacta gtaccggagc
     4021 tccattctgg tctgctccaa aaagattccc tcgtccactc cagtactcgt cgtccgaccc
     4081 aagtctcctt aactttatca cagcgactgc tattttacga gcagagacat ttgggatccc
     4141 gatacctgag tggactaaga acccaaagga agcagcagaa gctgttgaca gagtgatagt
     4201 cccagacttt gagccaaggc aagatgcaaa gattgtgact gatgagaaag ctaccacttt
     4261 aaccactgct tcggtggacg acgctgcagt catcgacgat ctcattgcta agatcgacca
     4321 gtgtaggcat aacttgtcgc cagacttcag gatgaaacct attcagttcg aaaaggatga
     4381 tgatacaaac tatcacatgg atgtgatagc aggactagcc aacatgaggg cgagaaatta
     4441 cagcatccct gaagtggaca agctgaaagc aaagttcata gcagggagaa tcatcccagc
     4501 cattgcgact tcaacagcca tggctactgg gctggtctgc ctcgagctgt acaaggtcct
     4561 tgatggagga cataaagtgg aagcttacag aaacacattt gccaacctgg cacttccact
     4621 attctccatg gctgaaccac ttccaccaaa ggtggtgaag caccgtgaca tggcttggac
     4681 agtttgggac agatgggttc tcaagggaaa cccaactctt cgtgaggtgt tgcagtggct
     4741 ggaggacaaa gggcttagcg catacagcat ctcttgtgga agctgtctac tgttcaacag
     4801 tatgttcaca aggcacaagg agaggatgga caagaaggtt gtggatctcg ctcgggatgt
     4861 tgcgaaagtc gagttaccgc cttatcgcaa ccatcttgat gtagtggtgg cttgtgagga
     4921 tgaagatgac aatgacgtcg atattcctct cgtctctatc tacttcaggt gagcttacta
     4981 ttaactttaa gaaggagata taccatgtac ccatacgatg ttcctgacta tgcgggctat
     5041 ccctatgacg tcccggacta tgcaggattg tatccatatg acgttccaga ttacgccact
     5101 agagctgctt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     5161 tatgcaggat tgtatccata tgacgttcca gattacgcaa tggcttcgaa acggatcttg
     5221 aaggagctga aggatctcca gaaagaccct ccaacctcct gcagtgcagg tccagttgct
     5281 gaggacatgt ttcattggca agctacaatt atgggtcctg cagagagtcc gtattcaggc
     5341 ggtgtgtttc tcgttaccat tcacttccct ccggactatc cattcaaacc accaaaggtt
     5401 gcatttagga cgaaggtgtt tcaccctaat atcaacagca acggaagcat ttgccttgac
     5461 attttgaaag aacaatggag ccctgccctc accatttcca aggtgttgct ctcgatatgt
     5521 tcgctgttaa cagatccaaa tccagatgac cctttggtac cagagattgc acacatgtac
     5581 aaaaccgaca gagccaaata cgaggctact gcaagaaact ggactcagaa gtatgccatg
     5641 ggctaagctt ttacttaact ttaagaagga gatataccat gtcccctata ctaggttatt
     5701 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
     5761 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
     5821 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
     5881 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
     5941 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
     6001 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
     6061 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
     6121 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
     6181 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     6241 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     6301 ccacgtttgg tggtggcgac catcctccaa aatcggatct ggttccgcgt ggatcaatgg
     6361 cgggtatcaa tagagcggga ccatcgggcg cttattttgt tggccacaca gaccccgagc
     6421 cagtatcggg gcaagcacac ggatccggca gcggcgccag ctcctcgaac agtccgcagg
     6481 ttcagccgcg accctcgaat actcccccgt cgaacgcgcc cgcaccgccg ccaaccggac
     6541 gtgagaggct ttcacgatcc acggcgctgt cgcgccaaac cagggagtgg ctggagcagg
     6601 gtatgcctac agcggaggat gccagcgtgc gtcgtaggcc acaggtgact gccgatgccg
     6661 caacgccgcg tgcagaggca agacgcacgc cggaggcaac tgccgatgcc agcgcaccgc
     6721 gtagaggggc ggttgcacac gccaacagta tcgttcagca attggtcagt gagggcgctg
     6781 atatttcgca tactcgtaac atgctccgca atgcaatgaa tggcgacgca gtcgcttttt
     6841 ctcgagtaga acagaacata tttcgccagc atttcccgaa catgcccatg catggaatca
     6901 gccgagattc ggaactcgct atcgagctcc gtggagcgct tcgtcgagcg gttcaccaac
     6961 aggcggcgtc agcgccagtg aggtcgccca cgccaacacc ggccagccct gcggcatcat
     7021 catcgggcag cagtcagcgt tctttatttg gacggtttgc ccgtttgatg gcgccaaacc
     7081 agggacggtc gtcgaacact gccgcctctc agacgccggt cgacaggagc ccgccacgcg
     7141 tcaaccaaag acccatacgc gtcgacaggg ctgcgatgcg taatcgtggc aatgacgagg
     7201 cggacgccgc gctgcggggg ttagtacaac agggggtcaa tttagagcac ctgcgcacgg
     7261 cccttgaaag acatgtaatg cagcgcctcc ctatccccct cgatataggc agcgcgttgc
     7321 agaatgtggg aattaaccca agtatcgact tgggggaaag ccttgtgcaa catcccctgc
     7381 tgaatttgaa tgtagcgttg aatcgcatgc tggggctgcg tcccagcgct gaaagagcgc
     7441 ctcgtccagc cgtccccgtg gctcccgcga ccgcctccag gcgaccggat ggtacgcgtg
     7501 caacacgatt gcgggtgatg ccggagcggg aggattacga aaataatgtg gcctatggag
     7561 tgcgcttgct taacctgaac ccgggggtgg gggtaaggca ggctgttgcg gcctttgtaa
     7621 ccgaccgggc tgagcggcca gcagtggtgg ctaatatccg ggcagccctg gaccctatcg
     7681 cgtcacaatt cagtcagctg cgcacaattt cgaaggccga tgctgaatct gaagagctgg
     7741 gttttaagga tgcggcagat catcacacgg atgacgtgac gcactgtctt tttggcggag
     7801 aattgtcgct gagtaatccg gatcagcagg tgatcggttt ggcgggtaat ccgacggaca
     7861 cgtcgcagcc ttacagccaa gagggaaata aggacctggc gttcatggat atgaaaaaac
     7921 ttgcccaatt cctcgcaggc aagcctgagc atccgatgac cagagaaacg cttaacgccg
     7981 aaaatatcgc caagtatgct tttagaatag tcccctgagc ttcagattaa ctttaagaag
     8041 gagatatacc atggaacaaa agttgatctc tgaagaggac cttgagcaga aattgatctc
     8101 tgaggaagat cttgagcaga agcttatctc agaggaggat ttggagcaaa aacttatttc
     8161 tgaagaggac ctaatgggaa gcaagtattc caaggcaaca aattcgataa atgatgcttc
     8221 taacttgagt tatggcgttc cttttgaaaa ttatcgagtt ccttttgtag atttggagga
     8281 agcaactaac aactttgatg acaatttttt cattggagag ggtggatttg ggaaggttta
     8341 caggggtgtt ttgcgtgatg gaacaaaggt cgccctgaaa aagcataaac ctgagtcctc
     8401 acaaggtatt gaagagttcg aaacagaaat tgagattctc tcattttgca gccatccgca
     8461 tctggtttca ttgataggat tctgtgatga aagaaatgag atgattctaa tttatgacta
     8521 catggagaat gggaacctca agagccattt gtatggatca gatctaccct ccatgagctg
     8581 ggagcagagg ctggagatat gtatcggggc agccagaggc cttcactacc ttcataagaa
     8641 tgcagttata catcgtgatg tcaagtgtac aaacatattg cttgatgaga attttgtgcc
     8701 aaaaattact gattttggaa tatctaagac aatgcccgag cttgaccaaa cccatcttag
     8761 cacagtagtg agaggaaata taggctacat tgcccctgaa tatgctttgt ggggacagct
     8821 gacagaaaaa tctgatgttt attctttcgg tgttgtttta ttcgaagttc tttgtgctag
     8881 gcctgcctta gatcgttcag agataatgag tttagatgat gagacgcaga agatgggaca
     8941 gttggaacaa attgtagatc ccactattgc ggccaaaata agaccagagt ccctcaggat
     9001 gtttggagaa acagcgatca aatgcttagc tccgtctagt aaaaataggc catcaatggg
     9061 tgatgtgctg tggaaactgg agtatgcact ttgtctccaa gagcctacca ttcaagatga
     9121 tcctgaatga gctttgtggc ttttaagcca gccccgacac ccgccaacac ccgctgacgc
     9181 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgggatgag
     9241 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     9301 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     9361 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9421 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9481 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9541 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9601 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9661 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9721 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9781 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9841 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9901 cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg
     9961 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
    10021 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
    10081 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
    10141 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
    10201 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
    10261 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc
    10321 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
    10381 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
    10441 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
    10501 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
    10561 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
    10621 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
    10681 taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat
    10741 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
    10801 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10981 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    11041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11521 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11581 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11641 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg
    11701 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    11761 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    11821 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgactc cgggagctgc
    11881 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
    11941 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
    12001 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
    12061 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
    12121 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
    12181 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
    12241 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
    12301 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
    12361 tccagacttt acgaaacacg gaaacccaag accattcatg ttgttgctca ggtcgcagac
    12421 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
    12481 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
    12541 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
    12601 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
    12661 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
    12721 acctgtccta cgagttgcat gataaagaac acagtcataa gtgcggcgac gatagtcatg
    12781 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
    12841 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
    12901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    12961 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgacacg
//
