LOCUS       sequence-205480-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-302
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     933..952
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(972..1049)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1255..1274
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1358..1377
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1358..1376
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1377..1401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1455..1472
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5020..5109
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5122..5211
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5817..5835)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5831..5878
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5915..6370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6002..6021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6212..6233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6462..7277)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6462..7277)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7185..7204
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7269..7288
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7399..7987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7888..7907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8141..8158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8173..8312
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8238..8257)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8398..8420
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8414..8605)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9414..9525,1..971))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 accagtgaca cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
       61 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
      121 gggatataac atgagctgtc ctcggtatcg tcgtatccca ctaccgagat atccgcacca
      181 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
      241 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
      301 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
      361 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
      421 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtcc
      481 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
      541 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
      601 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
      661 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
      721 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
      781 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
      841 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
      901 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
      961 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     1021 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
     1081 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
     1141 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     1201 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     1261 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     1321 tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa
     1381 ttgtgagcgg ataacaattc ccctctagaa ataattttgc cttaacttta agaaggagat
     1441 ataccatggg cagccaccac caccaccacc acagcggaat gcagatcttt gttaagactc
     1501 tcaccggaaa gacaatcacc ctagaggtgg aaagctccga caccatcgac aacgttaagg
     1561 ccaagatcca ggataaggag ggcattcctc cggatcagca gaggcttatt ttcgccggca
     1621 agcagctaga ggatggccgt acgttggctg attacaatat ccagaaggaa tccaccctcc
     1681 acttggtcct caggctccgt ggtggttaag cttgcaatta actttaagaa ggagatatac
     1741 aatgcttcac aagcgagcta gtgaagctaa cgataagaac gataatacca tcatcggttc
     1801 cgatttagca tcatctaaga aacgccgtat cgatttcact gaatcttcgt ctgataagtc
     1861 atcttcgatt ttagcttctg gtagtagcag gggttttcac ggcgatagcg tcgttcagca
     1921 aatcgacatg gcttttggta attcgaaccg tcaggagatt gatgaagatc tgcacagtcg
     1981 ccagctcgcc gtctatggtc gtgagactat gaggcgtctc tttgcttcca atgttctcat
     2041 ctcggggatg cacggtcttg gtgccgagat tgccaagaat cttatacttg ctggtgtgaa
     2101 gtctgtgacc ttgcatgatg aaagagttgt agagctatgg gacttatcaa gcaactttgt
     2161 tttctcggag gatgatgttg gcaagaatag agcagatgct tctgttcaga agttacagga
     2221 tcttaacaat gctgtggttg tctctagctt gaccaaaagc ttaaacaaag aggatctttc
     2281 tggcttccag gttgttgtat tctctgacat aagcatggaa agagcaatcg aatttgatga
     2341 ctattgtcac agccatcagc ctcctatcgc ttttgtcaag gctgatgtca ggggtctttt
     2401 cggctctgtc ttttgtgatt ttgggcctga atttgcggtt cttgatgttg atggagagga
     2461 accccataca ggcatcattg cgtctatctc taatgagaac caggcattta tttcctgtgt
     2521 cgacgatgag aggcttgagt ttgaagatgg tgacctagtt gttttctcag aagttgaggg
     2581 tatgacagaa ttgaatgatg ggaaaccaag gaagataaaa agtacacggc catattcatt
     2641 cacccttgat gaggacacta ctaactatgg aacatatgtg aagggtggta ttgtcactca
     2701 agtgaaacag ccaaagttac tgaatttcaa gccattgagg gaagcgctta aggatccagg
     2761 ggattttcta tttagtgatt tctctaagtt tgatcggcct ccactccttc acttagcatt
     2821 ccaggcactt gatcatttta aagctgaagc tggcagattt cctgttgctg gctcagaaga
     2881 ggacgctcag aagcttatat cgattgccac agccatcaat accgggcagg gtgatttaaa
     2941 ggtggagaat gttgatcaga agcttctaag acacttttcc tttggagcca aggctgtcct
     3001 gaatcctatg gctgcaatgt ttggcggtat tgttggacag gaggttgtta aagcttgctc
     3061 tggaaaattt catcctctct tccagttttt ctactttgat tcagtggagt cactcccctc
     3121 tgaacctgtg gactcgagtg actttgcacc aagaaacagc cggtatgatg cccaaatatc
     3181 tgtatttggg gccaagttcc agaagaaact tgaagatgct aaagttttca cagtagggtc
     3241 tggtgctctt ggctgtgagt tcttgaaaaa tttggctctg atgggggttt cttgtggaag
     3301 ccaagggaag ctgacagtga cagatgatga tatcattgag aagagtaacc tcagtcgtca
     3361 atttctgttc cgtgactgga acattggaca ggctaaatcc acagttgctg cttcggctgc
     3421 tgctgttata aatcctcgct tcaacattga ggccctgcaa aaccgtgttg gcgcggagac
     3481 tgagaatgta tttgacgatg ccttctggga gaacttaact gttgtcgtca atgcactgga
     3541 taatgttaat gcgaggctct atgttgattc gaggtgcttg tatttccaga agcctctcct
     3601 tgagtctgga actcttggta caaagtgcaa cacacagtcg gttatcccac atctaactga
     3661 aaattacggt gcctcaaggg acccaccaga gaaacaggcc cccatgtgta cagtgcattc
     3721 cttcccgcat aacattgatc actgtttaac ttgggctcgc tctgagtttg agggtctgct
     3781 ggagaaaact cccgctgaag tgaatgcata tctttctagc ccagttgagt acactaactc
     3841 aatgatgagt gctggtgatg ctcaggcgag ggacacattg gagaggattg ttgagtgcct
     3901 tgaaaaggag aagtgtgaaa ccttccaaga ctgcttaacc tgggcacgac tgaggtttga
     3961 ggattacttt gtgaaccgtg tcaagcaatt gatatacaca tttcctgaag atgctgcgac
     4021 tagtaccgga gctccattct ggtctgctcc aaaaagattc cctcgtccac tccagtactc
     4081 gtcgtccgac ccaagtctcc ttaactttat cacagcgact gctattttac gagcagagac
     4141 atttgggatc ccgatacctg agtggactaa gaacccaaag gaagcagcag aagctgttga
     4201 cagagtgata gtcccagact ttgagccaag gcaagatgca aagattgtga ctgatgagaa
     4261 agctaccact ttaaccactg cttcggtgga cgacgctgca gtcatcgacg atctcattgc
     4321 taagatcgac cagtgtaggc ataacttgtc gccagacttc aggatgaaac ctattcagtt
     4381 cgaaaaggat gatgatacaa actatcacat ggatgtgata gcaggactag ccaacatgag
     4441 ggcgagaaat tacagcatcc ctgaagtgga caagctgaaa gcaaagttca tagcagggag
     4501 aatcatccca gccattgcga cttcaacagc catggctact gggctggtct gcctcgagct
     4561 gtacaaggtc cttgatggag gacataaagt ggaagcttac agaaacacat ttgccaacct
     4621 ggcacttcca ctattctcca tggctgaacc acttccacca aaggtggtga agcaccgtga
     4681 catggcttgg acagtttggg acagatgggt tctcaaggga aacccaactc ttcgtgaggt
     4741 gttgcagtgg ctggaggaca aagggcttag cgcatacagc atctcttgtg gaagctgtct
     4801 actgttcaac agtatgttca caaggcacaa ggagaggatg gacaagaagg ttgtggatct
     4861 cgctcgggat gttgcgaaag tcgagttacc gccttatcgc aaccatcttg atgtagtggt
     4921 ggcttgtgag gatgaagatg acaatgacgt cgatattcct ctcgtctcta tctacttcag
     4981 gtgagcttac tattaacttt aagaaggaga tataccatgt acccatacga tgttcctgac
     5041 tatgcgggct atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca
     5101 gattacgcca ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat
     5161 gacgtcccgg actatgcagg attgtatcca tatgacgttc cagattacgc aatggcttct
     5221 aagaggatct taaaggagct caaggatctg cagaaggatc ctccttcaaa ctgcagcgct
     5281 ggtccggtgg ctgaggacat gttccattgg caagccacta tcatgggacc tcctgaaagt
     5341 ccgtatgccg gaggagtgtt tttggtttct attcatttcc ctccggatta tcctttcaag
     5401 ccaccaaagg tgtcttttaa gactaaggtg taccatccaa acatcaatag caatggaagt
     5461 atttgccttg atattttgaa agagcaatgg agccctgctc ttactatctc taaggttctg
     5521 ttgtcgattt gctcgctgtt gactgaccca aacccagatg atcctcttgt gccggagata
     5581 gctcacatgt acaagacaga tagatccaag tacgagtcaa ctgcgagaag ctggacacaa
     5641 aagtacgcaa tgggttgagc ttttacgctt ttaagccagc cccgacaccc gccaacaccc
     5701 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5761 gggatgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     5821 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     5881 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6121 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6241 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6361 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6421 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     6481 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     6541 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     6601 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     6661 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     6721 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     6781 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
     6841 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     6901 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     6961 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     7021 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     7081 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     7141 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     7201 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     7261 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     7321 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7381 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7441 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7501 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7561 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7621 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7681 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7741 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7801 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7861 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7921 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7981 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8041 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8101 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8161 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8221 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     8281 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     8341 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgactccg
     8401 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     8461 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     8521 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     8581 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     8641 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     8701 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     8761 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     8821 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     8881 tccgcgtttc cagactttac gaaacacgga aacccaagac cattcatgtt gttgctcagg
     8941 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     9001 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     9061 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     9121 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     9181 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     9241 ctgccggcac ctgtcctacg agttgcatga taaagaacac agtcataagt gcggcgacga
     9301 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     9361 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     9421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9481 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttc
//
