LOCUS       sequence-205536-        6354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses E. coli HPII catalase with TAG stop codon at site 283 and 
            N-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-HPII TAG283
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..300
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     223..242
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             346..363
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             367..399
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(403..411,412..426)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(2770..2787)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2940..3026
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3118..3145
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3164..3255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3256..3324,3325..4116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3474..3493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4287..4875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4776..4795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5029..5046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5061..5201
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5127..5146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5287..5309
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5465..6343)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
       61 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
      121 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
      181 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
      241 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
      301 acccgttttt tgggctaaca ggaggaatta accatggggg gttctcatca tcatcatcat
      361 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      421 gataaggatc gatggggatc cgagctcgag tcgcaacata acgaaaagaa cccacatcag
      481 caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc actggcacct
      541 gaggacggct ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca acctaccgcc
      601 ccagggagcc tgaaagcccc tgatacgcgt aacgaaaaac ttaattctct ggaagacgta
      661 cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat cgccgacgat
      721 caaaactcac tgcgtgccgg tagccgtggt ccaacgctgc tggaagattt tattctgcgc
      781 gagaaaatca cccactttga ccatgagcgc attccggaac gtattgttca tgcacgcgga
      841 tcagccgctc acggttattt ccagccatat aaaagcttaa gcgatattac caaagcggat
      901 ttcctctcag atccgaacaa aatcacccca gtatttgtac gtttctctac cgttcagggt
      961 ggtgctggct ctgctgatac cgtgcgtgat atccgtggct ttgccaccaa gttctatacc
     1021 gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat ccaggatgcg
     1081 cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc aattccacaa
     1141 gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga aactctgcac
     1201 aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac catggaaggc
     1261 ttcggtattc acaccttccg cctgattaat gcctagggga aggcaacgtt tgtacgtttc
     1321 cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca aaaactcacc
     1381 ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc aggcgatttt
     1441 ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa gttcgacttc
     1501 gatcttctcg atccaaccaa acttatcccg gaagaactgg tgcccgttca gcgtgtcggc
     1561 aaaatggtgc tcaatcgcaa cccggataac ttctttgctg aaaacgaaca ggcggctttc
     1621 catcctgggc atatcgtgcc gggactggac ttcaccaacg atccgctgtt gcagggacgt
     1681 ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt ccatgagatt
     1741 ccgattaacc gtccgacctg cccttaccat aatttccagc gtgacggcat gcatcgcatg
     1801 gggatcgaca ctaacccggc gaattacgaa ccgaactcga ttaacgataa ctggccgcgc
     1861 gaaacaccgc cggggccgaa acgcggcggt tttgaatcat accaggagcg cgtggaaggc
     1921 aataaagttc gcgagcgcag cccatcgttt ggcgaatatt attcccatcc gcgtctgttc
     1981 tggctaagtc agacgccatt tgagcagcgc catattgtcg atggtttcag ttttgagtta
     2041 agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc gcatattgat
     2101 ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga cgaccagctg
     2161 aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt aagtttgtac
     2221 gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa tgatgaagtg
     2281 agatcggcag accttctggc cattctcaag gcgctgaagg ccaaaggcgt tcatgccaaa
     2341 ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt gcctatagcc
     2401 gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc ttgcggcaat
     2461 atcgcggata tcgctgacaa cggcgatgcc aactactacc tgatggaagc ctacaaacac
     2521 cttaaaccga ttgcgctggc gggtgacgcg cgcaagttta aagcaacaat caagatcgct
     2581 gaccagggtg aagaagggat tgtggaagct gacagcgctg acggtagttt tatggatgaa
     2641 ctgctaacgc tgatggcagc acaccgcgtg tggtcacgca ttcctaagat tgacaaaatt
     2701 cctgcctgat ggggtaccat atgggaattc gaagcttggc tgttttggcg gatgagagaa
     2761 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     2821 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     2881 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     2941 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3001 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     3061 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     3121 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
     3181 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3241 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3301 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3361 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3421 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3481 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     3541 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3601 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3661 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3721 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3781 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3841 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3901 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3961 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4021 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4081 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4141 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4201 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4261 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4861 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4921 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4981 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5041 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5101 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5161 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     5221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     5341 agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg
     5401 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
     5461 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     5521 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     5581 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     5641 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     5701 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     5761 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     5821 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     5881 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     5941 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     6001 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     6061 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     6121 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     6181 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     6241 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     6301 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atac
//
