LOCUS       sequence-205559-       10012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-338
FEATURES             Location/Qualifiers
     source          1..10012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     564..583
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(603..680)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     886..905
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     989..1008
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        989..1007
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1008..1032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1086..1103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4753..4842
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5935..5953)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5949..5996
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6033..6488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6120..6139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6330..6351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6580..7395)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6580..7395)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7303..7322
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7387..7406
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7517..8105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8006..8025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8259..8276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8291..8430
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8356..8375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8516..8538
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8532..8723)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9532..10012,1..602))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
       61 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ctcatgggag
      121 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
      181 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
      241 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
      301 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
      361 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
      421 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
      481 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
      541 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
      601 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
      661 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
      721 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
      781 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
      841 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      901 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      961 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
     1021 gataacaatt cccctctaga aataattttg ccttaacttt aagaaggaga tataccatgg
     1081 gcagccacca ccaccaccac cacagcggaa tgcagatctt tgttaagact ctcaccggaa
     1141 agacaatcac cctagaggtg gaaagctccg acaccatcga caacgttaag gccaagatcc
     1201 aggataagga gggcattcct ccggatcagc agaggcttat tttcgccggc aagcagctag
     1261 aggatggccg tacgttggct gattacaata tccagaagga atccaccctc cacttggtcc
     1321 tcaggctccg tggtggttaa gcttgcaatt aactttaaga aggagatata caatgcttca
     1381 caagcgagct agtgaagcta acgataagaa cgataatacc atcatcggtt ccgatttagc
     1441 atcatctaag aaacgccgta tcgatttcac tgaatcttcg tctgataagt catcttcgat
     1501 tttagcttct ggtagtagca ggggttttca cggcgatagc gtcgttcagc aaatcgacat
     1561 ggcttttggt aattcgaacc gtcaggagat tgatgaagat ctgcacagtc gccagctcgc
     1621 cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc aatgttctca tctcggggat
     1681 gcacggtctt ggtgccgaga ttgccaagaa tcttatactt gctggtgtga agtctgtgac
     1741 cttgcatgat gaaagagttg tagagctatg ggacttatca agcaactttg ttttctcgga
     1801 ggatgatgtt ggcaagaata gagcagatgc ttctgttcag aagttacagg atcttaacaa
     1861 tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa gaggatcttt ctggcttcca
     1921 ggttgttgta ttctctgaca taagcatgga aagagcaatc gaatttgatg actattgtca
     1981 cagccatcag cctcctatcg cttttgtcaa ggctgatgtc aggggtcttt tcggctctgt
     2041 cttttgtgat tttgggcctg aatttgcggt tcttgatgtt gatggagagg aaccccatac
     2101 aggcatcatt gcgtctatct ctaatgagaa ccaggcattt atttcctgtg tcgacgatga
     2161 gaggcttgag tttgaagatg gtgacctagt tgttttctca gaagttgagg gtatgacaga
     2221 attgaatgat gggaaaccaa ggaagataaa aagtacacgg ccatattcat tcacccttga
     2281 tgaggacact actaactatg gaacatatgt gaagggtggt attgtcactc aagtgaaaca
     2341 gccaaagtta ctgaatttca agccattgag ggaagcgctt aaggatccag gggattttct
     2401 atttagtgat ttctctaagt ttgatcggcc tccactcctt cacttagcat tccaggcact
     2461 tgatcatttt aaagctgaag ctggcagatt tcctgttgct ggctcagaag aggacgctca
     2521 gaagcttata tcgattgcca cagccatcaa taccgggcag ggtgatttaa aggtggagaa
     2581 tgttgatcag aagcttctaa gacacttttc ctttggagcc aaggctgtcc tgaatcctat
     2641 ggctgcaatg tttggcggta ttgttggaca ggaggttgtt aaagcttgct ctggaaaatt
     2701 tcatcctctc ttccagtttt tctactttga ttcagtggag tcactcccct ctgaacctgt
     2761 ggactcgagt gactttgcac caagaaacag ccggtatgat gcccaaatat ctgtatttgg
     2821 ggccaagttc cagaagaaac ttgaagatgc taaagttttc acagtagggt ctggtgctct
     2881 tggctgtgag ttcttgaaaa atttggctct gatgggggtt tcttgtggaa gccaagggaa
     2941 gctgacagtg acagatgatg atatcattga gaagagtaac ctcagtcgtc aatttctgtt
     3001 ccgtgactgg aacattggac aggctaaatc cacagttgct gcttcggctg ctgctgttat
     3061 aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt ggcgcggaga ctgagaatgt
     3121 atttgacgat gccttctggg agaacttaac tgttgtcgtc aatgcactgg ataatgttaa
     3181 tgcgaggctc tatgttgatt cgaggtgctt gtatttccag aagcctctcc ttgagtctgg
     3241 aactcttggt acaaagtgca acacacagtc ggttatccca catctaactg aaaattacgg
     3301 tgcctcaagg gacccaccag agaaacaggc ccccatgtgt acagtgcatt ccttcccgca
     3361 taacattgat cactgtttaa cttgggctcg ctctgagttt gagggtctgc tggagaaaac
     3421 tcccgctgaa gtgaatgcat atctttctag cccagttgag tacactaact caatgatgag
     3481 tgctggtgat gctcaggcga gggacacatt ggagaggatt gttgagtgcc ttgaaaagga
     3541 gaagtgtgaa accttccaag actgcttaac ctgggcacga ctgaggtttg aggattactt
     3601 tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa gatgctgcga ctagtaccgg
     3661 agctccattc tggtctgctc caaaaagatt ccctcgtcca ctccagtact cgtcgtccga
     3721 cccaagtctc cttaacttta tcacagcgac tgctatttta cgagcagaga catttgggat
     3781 cccgatacct gagtggacta agaacccaaa ggaagcagca gaagctgttg acagagtgat
     3841 agtcccagac tttgagccaa ggcaagatgc aaagattgtg actgatgaga aagctaccac
     3901 tttaaccact gcttcggtgg acgacgctgc agtcatcgac gatctcattg ctaagatcga
     3961 ccagtgtagg cataacttgt cgccagactt caggatgaaa cctattcagt tcgaaaagga
     4021 tgatgataca aactatcaca tggatgtgat agcaggacta gccaacatga gggcgagaaa
     4081 ttacagcatc cctgaagtgg acaagctgaa agcaaagttc atagcaggga gaatcatccc
     4141 agccattgcg acttcaacag ccatggctac tgggctggtc tgcctcgagc tgtacaaggt
     4201 ccttgatgga ggacataaag tggaagctta cagaaacaca tttgccaacc tggcacttcc
     4261 actattctcc atggctgaac cacttccacc aaaggtggtg aagcaccgtg acatggcttg
     4321 gacagtttgg gacagatggg ttctcaaggg aaacccaact cttcgtgagg tgttgcagtg
     4381 gctggaggac aaagggctta gcgcatacag catctcttgt ggaagctgtc tactgttcaa
     4441 cagtatgttc acaaggcaca aggagaggat ggacaagaag gttgtggatc tcgctcggga
     4501 tgttgcgaaa gtcgagttac cgccttatcg caaccatctt gatgtagtgg tggcttgtga
     4561 ggatgaagat gacaatgacg tcgatattcc tctcgtctct atctacttca ggtgagctta
     4621 ctattaactt taagaaggag atataccatg tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga ttgtatccat atgacgttcc agattacgcc
     4741 actagagctg cttacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg
     4801 gactatgcag gattgtatcc atatgacgtt ccagattacg caatggccaa tagtaatctt
     4861 ccccgacgaa tcatcaagga aactcaacgt ctgcttagtg aacctgctcc tgggataagt
     4921 gcgtctccat cagaggaaaa tatgagatat ttcaatgtta tgattcttgg tcctactcaa
     4981 tctccttatg aaggaggagt tttcaagttg gagctctttt tgcctgaaga atatcctatg
     5041 gcagctccca aggttaggtt tctcaccaag atttaccatc ctaacatcga caagcttgga
     5101 agaatctgcc ttgacatact gaaagacaag tggagtcctg cactacaaat acgaactgtg
     5161 ctcttgagta tccaagctct tttaagtgca ccaaacccag atgatcctct gtctgagaac
     5221 atagctaagc attggaagag caatgaggca gaagctgtgg aaacagctaa ggaatggact
     5281 cgtctttacg caagtggtgc ttaagctttt acttaacttt aagaaggaga tatacaatga
     5341 ctcttggctc aggaggatct agcgttgttg ttccgaggaa ttttcggttg ttggaggagc
     5401 ttgaacgtgg ggagaaaggt attggggatg gaactgtgag ctatggaatg gatgatggag
     5461 atgacatcta tatgcgttct tggactggca ccatcatcgg tcctcacaac actgttcatg
     5521 aaggtagaat ctatcagttg aagctctttt gtgacaaaga ttacccggag aaacctccaa
     5581 ctgtccgatt ccattcacgt gtcaacatgg cttgtgtcaa ccacgaaaca ggagtggtgg
     5641 atcctaagaa atttgggcta cttgcaaact ggcaaagaga gtacacaatg gaggatatac
     5701 tggtacagtt gaagaaggaa atgtctacgt cgcataaccg taagcttgtt caacctccgg
     5761 aaggtacttg cttctaggct tcagagcttt taagccagcc ccgacacccg ccaacacccg
     5821 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacgg
     5881 gatgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     5941 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6001 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6181 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6241 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6361 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6481 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6541 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
     6601 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     6661 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     6721 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     6781 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     6841 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     6901 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
     6961 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     7021 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     7081 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     7141 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     7201 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     7261 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     7321 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
     7381 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     7441 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7501 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7561 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7621 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7681 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7741 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7801 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7861 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7921 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7981 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8041 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8101 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8161 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8221 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8281 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8341 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     8401 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8461 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgactccggg
     8521 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     8581 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     8641 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     8701 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     8761 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     8821 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     8881 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     8941 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     9001 cgcgtttcca gactttacga aacacggaaa cccaagacca ttcatgttgt tgctcaggtc
     9061 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     9121 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     9181 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     9241 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     9301 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     9361 gccggcacct gtcctacgag ttgcatgata aagaacacag tcataagtgc ggcgacgata
     9421 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     9481 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     9541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9601 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg acacgggcaa
     9661 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     9721 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     9781 gtcctcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     9841 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     9901 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     9961 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gc
//
