LOCUS       sequence-205562-       12139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation A331T conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTA331T-myc
FEATURES             Location/Qualifiers
     source          1..12139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      873..2662
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2964..4883)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGGS
                     GGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPG
                     RRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRRK
                     KGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYN
                     LRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDA
                     IKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGE
                     EGQE"
     primer_bind     complement(5318..5336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5404..5508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5509..5577,5578..6369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5727..5746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6540..7128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7029..7048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7559..7704
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7747..8784
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8782..8801)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8826..8874
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9528..9907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9908..10111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10062..10082
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10108..10132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10160..10169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10835..10864
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
      481 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
      541 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
      601 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
      661 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
      721 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
      781 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
      841 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
      901 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
      961 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     1021 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     1081 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     1141 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     1201 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     1261 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     1321 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     1381 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     1441 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     1501 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     1561 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     1621 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     1681 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     1741 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     1801 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     1861 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     1921 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     1981 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     2041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     2101 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     2161 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     2221 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     2281 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     2341 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2401 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     2461 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2521 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     2581 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt
     2641 ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca
     2701 ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg
     2761 cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc
     2821 atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca
     2881 tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg
     2941 agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct
     3001 ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga
     3061 aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag
     3121 gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat
     3181 atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt
     3241 tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat
     3301 tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca
     3361 tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta
     3421 gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga
     3481 ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg
     3541 ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg
     3601 ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc
     3661 cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat
     3721 gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc
     3781 ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca
     3841 ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga
     3901 ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
     3961 cctcctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     4021 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     4081 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     4141 cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     4201 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct
     4261 cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
     4321 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     4381 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
     4441 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     4501 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     4561 cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt
     4621 ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca
     4681 tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt
     4741 cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg
     4801 ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct
     4861 gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta
     4921 gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc
     4981 ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga
     5041 accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc
     5101 aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga
     5161 cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga
     5221 tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga
     5281 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5341 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5401 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5461 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5521 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5581 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5641 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5701 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     5761 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5821 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5881 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5941 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6001 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6061 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6661 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7141 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
     7201 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     7261 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
     7321 agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     7381 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     7441 ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg
     7501 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
     7561 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
     7621 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
     7681 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa
     7741 tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     7801 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     7861 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     7921 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     7981 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     8041 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     8101 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     8161 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     8221 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     8281 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     8341 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     8401 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     8461 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     8521 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     8581 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     8641 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     8701 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggat
     8761 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     8821 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     8881 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     8941 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     9001 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg
     9061 gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc
     9121 gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg
     9181 catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc
     9241 cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat
     9301 gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa
     9361 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
     9421 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
     9481 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
     9541 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9601 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9661 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9721 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9781 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9841 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9901 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     9961 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    10021 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    10081 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag
    10141 ctgggtacca gctgctagcg ccaccatgga gaccaccccc ctgaacagtc aaaagcaact
    10201 ctctgcctgt gaggatggag aggactgtca ggagaatggc gttcttcaaa aggttgtacc
    10261 cacccctgga gataaggtgg aaagcggcca gatttccaac ggctacagcg ccgtgccatc
    10321 acctggagcc ggggatgata cacggcacag cattcccgca accaccacga cactggttgc
    10381 agagcttcac cagggcgaga gggagacgtg gggcaaaaag gtagattttc tgctttccgt
    10441 gatcggttac gcggtcgatc tggggaacgt gtggaggttc ccttacattt gttaccaaaa
    10501 cggagggggt gccttcttgc tgccctacac aattatggcg atttttgggg gtatcccgct
    10561 cttctacatg gaactggcct tgggtcagta ccaccggaac ggctgcatta gcatttggcg
    10621 caagatatgc cccattttca agggaatcgg atacgcaatc tgtataatcg cgttctatat
    10681 tgcatcatat tacaacacca tcatggcctg ggccctttat tacttgatca gcagtttcac
    10741 cgaccaattg ccgtggacga gctgtaagaa ctcatggaat accgggaact gtaccaacta
    10801 cttctctgag gacagctcag gaggtagcgg gggtgaacag aagctgatta gcgaggagga
    10861 cttgggcagt ccaggcggaa gcggcaacat cacttggaca ctccatagta cgagccccgc
    10921 agaggagttt tacaccaggc acgtactgca gattcatagg agcaagggtc tgcaagacct
    10981 gggcggtatt agctggcagc tcgcattgtg cataatgttg atctttacgg tgatctattt
    11041 ctctatctgg aagggagtaa agacatccgg caaggtggtg tgggtgacgg cgaccttccc
    11101 ctacattata ctcagcgtgt tgttggtgag gggggcaacc ctgcctgggg cctggcgagg
    11161 cgtgctgttc tatctcaagc caaactggca gaagcttctt gaaaccggag tgtggatcga
    11221 cgcggccacg caaatcttct tttccctcgg tcccggcttc ggggtccttc tggcattcgc
    11281 aagctacaac aagttcaaca ataactgcta ccaggacgcc ctcgtgacaa gtgtagtgaa
    11341 ctgcatgacc tctttcgtga gcgggtttgt gatcttcaca gtactcggat atatggctga
    11401 gatgaggaac gaagatgtta gtgaggttgc caaagacgct ggcccgagcc tgttgttcat
    11461 cacatacgcc gaggctatcg cgaacatgcc cgcatctact ttcttcgcca tcatcttttt
    11521 cctgatgctg ataacccttg ggctggatag caccttcgcc ggactggagg gtgtcatcac
    11581 tgccgttctc gacgagttcc cccatgtgtg ggccaagcga cgggaaaggt tcgtgctggc
    11641 cgtcgtaatc acatgcttct tcggctcact ggtgaccctt acctttggcg gtgcgtacgt
    11701 ggtcaagctg ctcgaagagt acgccactgg accggcggtt ctgaccgtgg cattgatcga
    11761 ggccgtggcc gtaagctggt tttacggcat cacgcagttc tgtagggacg tgaaagagat
    11821 gctgggcttt tctcccggtt ggttttggag gatttgttgg gtagcaatta gccccctgtt
    11881 tctccttttc attatttgct cttttctgat gtctccaccg cagctccgac tgtttcagta
    11941 caactacccc tactggtcaa ttatcctggg ctattgtatc ggcacctcaa gtttcatctg
    12001 catccccaca tacatcgcat acagactgat aattacgccc ggtactttta aggagcgaat
    12061 cattaaaagc ataacccccg aaacccccac cgagatccct tgtggcgata taagactcaa
    12121 tgcggtctaa tagctcgag
//
