LOCUS       sequence-205605-        2283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nucleus targetting gTEMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y, Nagai T
  TITLE     Genetically encoded ratiometric fluorescent thermometer with wide 
            range and rapid response.
  JOURNAL   PLoS One. 2017 Feb 17;12(2):e0172344. doi: 
            10.1371/journal.pone.0172344. eCollection 2017.
  PUBMED    28212432
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-02-17"; volume: "12(2):e0172344. doi"; pages: " 
            10.1371/journal.pone.0172344. eCollection 2017"
COMMENT     Sequence Label: nuc-gTEMP_pcDNA3
FEATURES             Location/Qualifiers
     source          1..2283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             466..717
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDEPYK"
     CDS             1120..1173
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1180..1182,1183..1185,1186..1896)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgcagtt cggcgtgctg tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacgggat aagctcaaac gtatatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagcg tccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt ccgtgaccgc cgccgggatc actctcggca tggacgagcc gtacaaggaa
      721 ttcatgccgg agccagcgaa gtctgctccc gcgcccaaga agggctcgaa gaaagccgtg
      781 actaaggcgc agaagaagga cggcaagaag cgcaagcgca gccgcaagga gagctactcc
      841 gtatacgtgt acaaggtgct gaagcaggtc caccccgaca ccggcatctc ctctaaggcc
      901 atgggaatca tgaactcctt cgtcaacgac atcttcgaac gcatcgcggg tgaggcttcc
      961 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg
     1021 cgcctgctgc tgcccgggga gttggccaag cacgccgtgt ccgagggcac caaggccgtc
     1081 accaagtaca ccagcgctaa gcgcatgcat ggcagcggcg agggcagagg cagcctgctg
     1141 acctgcggcg acgtggagga gaaccccggc cccgagctca tggtgagcaa gggcgaggag
     1201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttctcctac
     1381 ggcgtgatgg tgttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac
     1621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggc caacttcaag
     1681 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     1741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcat ccagtccaag
     1801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1861 gccgggatca ctctcggcat ggacgagctg tacaaggaat tcatgccgga gccagcgaag
     1921 tctgctcccg cgcccaagaa gggctcgaag aaagccgtga ctaaggcgca gaagaaggac
     1981 ggcaagaagc gcaagcgcag ccgcaaggag agctactccg tatacgtgta caaggtgctg
     2041 aagcaggtcc accccgacac cggcatctcc tctaaggcca tgggaatcat gaactccttc
     2101 gtcaacgaca tcttcgaacg catcgcgggt gaggcttccc gcctggcgca ttacaacaag
     2161 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcccggggag
     2221 ttggccaagc acgccgtgtc cgagggcacc aaggccgtca ccaagtacac cagcgctaag
     2281 taa
//
