LOCUS       sequence-205606-       10220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  single sgRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, 
            Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
  TITLE     Dual gene activation and knockout screen reveals directional 
            dependencies in genetic networks.
  JOURNAL   Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.
  PUBMED    29334369
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062."
COMMENT     Sequence Label: sgLenti
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..48)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        220..599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        601..799
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     754..774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             817..997
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1044..1169
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1666..1899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2084..2128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2426..2543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2796..2829)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2833..2853
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4431..4642
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4643..5581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5538..5558
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5602..6198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5602..5621)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6098..6118
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6208..6261
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6268..6277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6274..6981
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6419..6437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6687..6706
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6991..7011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7091..7679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7144..7164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7562..7573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7682..7698)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7683..7699)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8208..8388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8450..9038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8530..8549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9209..10000,10001..10069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9832..9851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10070..10174)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
       61 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
      121 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
      181 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      301 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      361 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      421 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      481 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      541 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      601 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      661 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      721 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      781 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      841 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      901 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      961 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1021 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1081 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1141 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1201 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1261 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1321 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1381 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1441 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1501 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1561 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1621 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1681 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1741 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1801 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1861 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1921 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1981 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2041 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2101 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2161 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2221 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2281 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2341 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     2401 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2461 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2521 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     2581 tagtaccggg cccgctctag agatccgacg cgccatctct aggcccgcgc cggccccctc
     2641 gcacggactt gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat
     2701 ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat
     2761 actagctaca ttttacatga taggcttgga tttctataac ttcgtatagc atacattata
     2821 cgaagttata aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg
     2881 agactataag tatcccttgg agaaccacct tgttggagac gcaggtgtca ctggcgctgt
     2941 tggtgccggc acctgacaca gcagaggatg cagagcccaa ggaaacccag aaagccgaag
     3001 gagccaccca gcacgcaagc caggaacact gtctggttca cccctctgga taaaacctgg
     3061 atcctggagt ccatcttgac cttatcacct tcactcagta gacactgcca cagccctgtc
     3121 tcaggggcca ccacttgaac tactttctgc tcctcagaga ccctggcctc ctggttctcc
     3181 tgcttcaggg tcagtctcat cttgggagag gtaggtccca tcacctcaca ggtcaaagta
     3241 ttgttgagct gagccacttt catcaccacc aggttcactt cctgatgcag tgtccctttg
     3301 tccagagtca gagtcaggtt gccagaacca gcaaactgaa gcgagacctg gggtatcttg
     3361 agggtgagtg ggagcgtttc cttcagctgg agcttgaggt ctttggtgga cttttgtacg
     3421 gacacctctt tgttctttat ggagaaggag atccagggct ggaagaaaga atccttctct
     3481 gccttccaca tcagctctcc ccacccgttt tcctctgcaa agttgagtgg gaaggagaac
     3541 tccgctgact ctccctcact cttataggcc gtgatagctg tgctctgaaa acccagcact
     3601 gagagtgtca tgccgaacca gttctttttc tggtccaggg tcacggtgca gttccagaag
     3661 tcgctgtcct gaacccttag gttggacatg gagagaactt tggaaccact gacaacttta
     3721 ccctttttgt gtttgcactc tgtcaagggg ttagagacct tagagttgct atccaaggtc
     3781 agggtcaggc tctgcccttg caacaggctg gtacccggac tgaaggtcac tttgaacacc
     3841 cacaactcca cctcctcttt cctgttctcc agctcacaga tataagtctg agagtcttcc
     3901 atcttaagtt tattgatgat gagaggaaac gatcctttct cccatgcccc ttttttggaa
     3961 tcaaaacgat caaactgcga aggcgaacct cctctaatta atacaccttt gccatgctgc
     4021 cccagaatct tcctctggtc agagaacttc caggtgaaga ctgtgatctt cttctgggaa
     4081 ctctcgcagg gcagttctgc tgattcccct tccttcccca gcaccagcgt cttcccttga
     4141 gtgacagcta ggagttgtga cagctgcagc agcagcagca gcaagcgcct aagagagatg
     4201 gctcggcaca tgtcacctgc gatcgtttaa gagctatgct ggaaacagca tagcaagttt
     4261 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgcg
     4321 gccgcgtgga taaccgtatt accgccatgc atggtcacag actagcgtag gggttgcaga
     4381 tcggaagagc acacgcgtct gaactccagt cactgcatgt gcccgtcagt gggcagagcg
     4441 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     4501 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4561 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4621 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     4681 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     4741 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     4801 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     4861 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     4921 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     4981 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     5041 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     5101 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     5161 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     5221 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     5281 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     5341 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg cgcttttgga gtacgtcgtc
     5401 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     5461 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     5521 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     5581 ggtgtcgtga gctagcccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5641 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5701 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5761 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5821 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5881 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5941 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6001 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6061 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6121 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6181 acccgcaagc ccggtgccgg atcgggagag ggcagaggaa gtctgctaac atgcggtgac
     6241 gtcgaggaga atcctggccc accggtcgcc accatggtga gcaagggcga ggaggataac
     6301 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     6361 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     6421 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     6481 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     6541 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     6601 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     6661 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     6721 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     6781 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     6841 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     6901 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     6961 ggcatggacg agctgtacaa gggagctgat ccaaaaaaga agagaaaggt atagttaatt
     7021 aagtcgaggg acctaacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
     7081 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7141 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7201 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7261 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7321 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7381 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7441 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7501 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7561 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7621 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7681 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7741 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7801 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7861 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7921 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7981 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8041 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     8101 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     8161 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     8221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa
     8401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8881 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10201 acgtcgacgg atcgggagat
//
