LOCUS       sequence-205637-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SDC3, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) SDC3
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgactc tagtccagtg tggtggaatt ctgcagatat caacaagttt
      121 gtacaaaaaa gcaggcttca ccatgaaacc aggtcctccc catcgagcgg gagcagcgca
      181 cggcgcggga gccggggcag gtgctgcagc tgggcccggt gcaagggggt tactcctccc
      241 tccactcctt ctattacttc ttgcgggaag agctgcaggt gctcagagat ggagatctga
      301 gaacttcgag agacccgtag atttagaggg tagtggtgac gacgatagct ttcccgatga
      361 tgagcttgat gatttgtatt ccgggagcgg gagcgggtac ttcgaacagg aaagcggcat
      421 agagacggct atgcggttta gtcccgacgt cgcgctagcg gtgtccacaa ctcctgcagt
      481 tctaccgact accaatatac agcctgtcgg gacgcctttc gaagaactgc cgtcggaaag
      541 acccactttg gagccagcaa catctcccct tgtggtaacg gaggtgccag aggagccatc
      601 acaacgggcc accacagtga gcacaacgat ggcaacgacc gcagcaacct ctaccgggga
      661 tccaacagta gcgactgtcc cagccacggt ggctacggca acaccctcta cacccgccgc
      721 ccctccgttc acagcaacta ccgccgttat tcgtaccaca ggagtccggc gactgctgcc
      781 actgcccctc acaaccgtgg caacagcccg tgctactacc cccgaagctc cttccccgcc
      841 tactactgct gccgtctt
//
