LOCUS       sequence-205646-        8768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FITM2, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) FITM2
FEATURES             Location/Qualifiers
     source          1..8768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     515..534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     768..785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    902..923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(938..943,944..961,962..968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    976..992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     981..1003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1000..1016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1000..1016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1035..1055
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1037..1055
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1083..1309
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1310..1490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1537..1662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2155..2388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2573..2617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2884..3000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3023..3326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3327..3530
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3481..3501
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3527..3551
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3635..3659
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4455..4479)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4510..5098
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4563..4583)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4981..4992)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5112..5611
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5147..5170)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5544..5563
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5632..6231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5632..5651)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6128..6148
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6369..6602
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6674..6795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6711..6730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6765..6784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6835..6970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6990..7009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6991..7009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7019..7036)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7019..7035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7028..7050)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7177..7632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7264..7283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7474..7495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7658..7762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7763..7831,7832..8623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7981..8000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
       61 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     1021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     1081 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     1141 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     1201 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     1261 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     1321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1501 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1561 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1621 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1681 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1741 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1801 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1861 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1921 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1981 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     2041 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     2101 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     2161 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     2221 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     2281 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     2341 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     2401 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     2461 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2521 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2581 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2641 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2701 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2761 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2821 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     2881 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2941 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     3001 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3061 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3121 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3181 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3241 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3301 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3361 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3421 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3481 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     3541 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     3601 tctagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt
     3661 caccatggaa cacttagagc ggtgcgagtg gttgttaaga ggtacactgg tccgagcggc
     3721 tgtgaggcgc tatttgcctt gggcccttgt agcctccatg cttgccggat ctctattgaa
     3781 agaacttagc ccccttccgg aatcatattt atctaataag cgcaatgtat tgaacgttta
     3841 tttcgttaaa gttgcctggg cgtggacgtt ttgcttgctg ctgcctttta tagcactaac
     3901 gaattaccac ctaacaggca aggcaggcct tgtcctcagg cggctgtcta ccctcctcgt
     3961 cggcaccgcc atttggtaca tatgtacaag tatcttttcc aacattgagc attatactgg
     4021 cagctgctat caaagtccag cacttgaagg tgtgagaaag gaacatcaat cgaagcagca
     4081 gtgccatcag gagggcggtt tttggcacgg atttgacatc tccggacact catttctcct
     4141 caccttctgc gctctgatga ttgtggagga gatgagcgtg ctgcatgagg tcaaaaccga
     4201 tcgttcccac tgtctgcaca cagctatcac aactctcgtg gtggcactcg gaatcctcac
     4261 tttcatctgg gtgctgatgt tcctgtgtac tgccgtgtac ttccataacc tgagccagaa
     4321 agtgtttggg accctgttcg ggctgctgtc ttggtacggc acctacgggt tctggtaccc
     4381 aaaggctttc tcccccgggc tgccccctca gtcatgtagc ctgaacctga agcaggacag
     4441 ttacaagaaa tgaaacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
     4501 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4561 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4621 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4681 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4741 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4801 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4861 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4921 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4981 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5041 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5101 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     5161 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     5221 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     5281 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     5341 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     5401 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5461 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5521 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5581 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     5641 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5701 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5761 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5821 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5881 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5941 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6001 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6061 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6121 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6181 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     6241 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     6301 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6361 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     6421 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6481 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6541 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6601 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6661 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6781 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     6841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6961 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7021 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7081 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7141 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7381 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7621 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8761 gtcagacc
//
