LOCUS       sequence-205651-        8655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eGFP, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) eGFP
FEATURES             Location/Qualifiers
     source          1..8655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      39..627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     528..547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     781..798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    915..936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(951..956,957..974,975..981)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    989..1005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     994..1016
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1013..1029
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1013..1029
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1048..1068
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1050..1068
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1096..1322
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1323..1503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1550..1675
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2168..2401
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2586..2630
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2928..3176
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2928..2948
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3099..3118
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        3331..3710
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3711..3914
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3865..3885
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3911..3935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4009..4011,4012..4014,4015..4728)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4009..4011,4012..4014,4015..4725)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4054..4075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4315..4334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4662..4683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4745..4769)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4844..4961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5010..5520
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5439..5457
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5542..6141
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5542..5561)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6038..6058
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6269..6502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6574..6695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6611..6630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6665..6684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6735..6870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6890..6909)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6891..6909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6919..6936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6919..6935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6928..6950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7077..7532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7164..7183)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7374..7395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7558..7662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7663..7731,7732..8523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7881..7900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
       61 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      181 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      781 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      841 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      901 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      961 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     1021 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     1081 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     1141 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     1201 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     1261 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     1321 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1501 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1561 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1621 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1681 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1741 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1801 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1861 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1921 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1981 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     2041 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     2101 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     2161 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     2221 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     2281 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     2341 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2401 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2461 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2521 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2581 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2641 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2701 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2761 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2821 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     2881 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc
     2941 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     3001 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     3061 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     3121 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggta
     3181 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     3241 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     3301 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
     3361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     3601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     3661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3721 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3781 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3841 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3901 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3961 ttgacctcca tagaagattc tagattcgaa tcgcgagaat tcggtaccat ggtgagcaag
     4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtagct cgagtgcggc
     4741 cgcaacccag ctttcttgta caaagtggtt gatcgcgtgc atgcgacgtc atagctctct
     4801 cccaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     4861 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     4921 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     4981 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
     5041 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     5101 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     5161 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     5221 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     5281 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     5341 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     5401 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     5461 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     5521 ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5581 gacgacgtcc ccagggcggt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5641 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5701 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5761 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5821 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5881 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5941 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6001 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6061 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6121 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     6181 gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6241 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6301 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6361 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6421 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6481 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6541 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6721 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6781 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6841 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6901 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6961 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7021 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7501 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     7561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8041 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8281 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8341 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8401 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8521 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8641 gagttttcgt tccac
//
