LOCUS       sequence-205652-       11490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation F263C with approximately
            wildtype activity, decreased surface expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTF263C-myc
FEATURES             Location/Qualifiers
     source          1..11490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(703..721)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        789..893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(894..962,963..1754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1112..1131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1925..2513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2414..2433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2944..3089
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3132..4169
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4167..4186)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4211..4259
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4913..5292
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5293..5496
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5447..5467
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5493..5517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5545..5554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6220..6249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7538..7672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7562..7581)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7616..7635
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7815..7837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8397..10186
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
       61 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
      121 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
      181 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
      241 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
      301 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
      361 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
      421 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
      481 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
      541 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
      601 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
      661 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
      721 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      781 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      901 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      961 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1021 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1081 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1141 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1201 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1261 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1321 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1381 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     1441 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1501 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1561 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1621 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1681 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1741 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1801 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1861 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1921 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1981 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2041 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2101 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2161 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2221 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2281 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2341 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2401 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2461 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2521 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt
     2581 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
     2641 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
     2701 agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     2761 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     2821 tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat ccccgtggcc
     2881 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
     2941 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
     3001 tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
     3061 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg
     3121 ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     3181 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     3241 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     3301 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     3361 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     3421 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     3481 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     3541 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     3601 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     3661 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     3721 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     3781 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     3841 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     3901 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     3961 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     4021 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     4081 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     4141 cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4201 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     4261 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4321 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4381 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg
     4441 ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc
     4501 gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc
     4561 ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac
     4621 acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt
     4681 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     4741 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     4801 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     4861 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     4921 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4981 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5041 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5101 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5161 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5221 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     5281 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     5341 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     5401 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     5461 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
     5521 agaagctggg taccagctgc tagcgccacc atggagacca cccccctgaa cagtcaaaag
     5581 caactctctg cctgtgagga tggagaggac tgtcaggaga atggcgttct tcaaaaggtt
     5641 gtacccaccc ctggagataa ggtggaaagc ggccagattt ccaacggcta cagcgccgtg
     5701 ccatcacctg gagccgggga tgatacacgg cacagcattc ccgcaaccac cacgacactg
     5761 gttgcagagc ttcaccaggg cgagagggag acgtggggca aaaaggtaga ttttctgctt
     5821 tccgtgatcg gttacgcggt cgatctgggg aacgtgtgga ggttccctta catttgttac
     5881 caaaacggag ggggtgcctt cttgctgccc tacacaatta tggcgatttt tgggggtatc
     5941 ccgctcttct acatggaact ggccttgggt cagtaccacc ggaacggctg cattagcatt
     6001 tggcgcaaga tatgccccat tttcaaggga atcggatacg caatctgtat aatcgcgttc
     6061 tatattgcat catattacaa caccatcatg gcctgggccc tttattactt gatcagcagt
     6121 ttcaccgacc aattgccgtg gacgagctgt aagaactcat ggaataccgg gaactgtacc
     6181 aactacttct ctgaggacag ctcaggaggt agcgggggtg aacagaagct gattagcgag
     6241 gaggacttgg gcagtccagg cggaagcggc aacatcactt ggacactcca tagtacgagc
     6301 cccgcagagg agttttacac caggcacgta ctgcagattc ataggagcaa gggtctgcaa
     6361 gacctgggcg gtattagctg gcagctcgca ttgtgcataa tgttgatctg cacggtgatc
     6421 tatttctcta tctggaaggg agtaaagaca tccggcaagg tggtgtgggt gacggcgacc
     6481 ttcccctaca ttatactcag cgtgttgttg gtgagggggg caaccctgcc tggggcctgg
     6541 cgaggcgtgc tgttctatct caagccaaac tggcagaagc ttcttgaaac cggagtgtgg
     6601 atcgacgcgg ccgcgcaaat cttcttttcc ctcggtcccg gcttcggggt ccttctggca
     6661 ttcgcaagct acaacaagtt caacaataac tgctaccagg acgccctcgt gacaagtgta
     6721 gtgaactgca tgacctcttt cgtgagcggg tttgtgatct tcacagtact cggatatatg
     6781 gctgagatga ggaacgaaga tgttagtgag gttgccaaag acgctggccc gagcctgttg
     6841 ttcatcacat acgccgaggc tatcgcgaac atgcccgcat ctactttctt cgccatcatc
     6901 tttttcctga tgctgataac ccttgggctg gatagcacct tcgccggact ggagggtgtc
     6961 atcactgccg ttctcgacga gttcccccat gtgtgggcca agcgacggga aaggttcgtg
     7021 ctggccgtcg taatcacatg cttcttcggc tcactggtga cccttacctt tggcggtgcg
     7081 tacgtggtca agctgctcga agagtacgcc actggaccgg cggttctgac cgtggcattg
     7141 atcgaggccg tggccgtaag ctggttttac ggcatcacgc agttctgtag ggacgtgaaa
     7201 gagatgctgg gcttttctcc cggttggttt tggaggattt gttgggtagc aattagcccc
     7261 ctgtttctcc ttttcattat ttgctctttt ctgatgtctc caccgcagct ccgactgttt
     7321 cagtacaact acccctactg gtcaattatc ctgggctatt gtatcggcac ctcaagtttc
     7381 atctgcatcc ccacatacat cgcatacaga ctgataatta cgcccggtac ttttaaggag
     7441 cgaatcatta aaagcataac ccccgaaacc cccaccgaga tcccttgtgg cgatataaga
     7501 ctcaatgcgg tctaatagct cgaggccggc aaggccggat ccagacatga taagatacat
     7561 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7621 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     7681 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     7741 gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt
     7801 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7861 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7921 ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga
     7981 ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt
     8041 acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg
     8101 gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg
     8161 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     8221 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     8281 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     8341 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg
     8401 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     8461 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     8521 aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc
     8581 cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca
     8641 gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa
     8701 ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac
     8761 gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata
     8821 taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat
     8881 ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac
     8941 acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa
     9001 gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca
     9061 aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg
     9121 aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt
     9181 tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg
     9241 actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa
     9301 accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc
     9361 gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct
     9421 gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg
     9481 ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     9541 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     9601 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     9661 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     9721 tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa
     9781 tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     9841 tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     9901 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     9961 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
    10021 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca
    10081 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
    10141 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
    10201 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
    10261 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
    10321 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
    10381 ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg
    10441 aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct
    10501 tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg
    10561 gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg
    10621 cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc
    10681 gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca
    10741 atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga
    10801 cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg
    10861 taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg
    10921 gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca
    10981 aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc
    11041 ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg
    11101 gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg
    11161 tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg
    11221 ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt
    11281 tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca
    11341 cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga
    11401 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
    11461 cctgcccctc ctcctgctcc tgcccctcct
//
