LOCUS       sequence-205659-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing RALB, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) RALB
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    114..138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(766..790)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(874..894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagtccagt gtggtggaat tctgcagata tcaacaagtt
      121 tgtacaaaaa agcaggcttc accatggctg ccaacaagag taagggccag agctccttgg
      181 ccctccacaa ggtgatcatg gttggcagcg gaggcgttgg caagtcagcc ctgacgcttc
      241 agttcatgta tgacgagttt gtagaagact atgaacctac caaagctgac agttatagaa
      301 agaaagtggt tcttgatggg gaagaagttc agatagatat tctggacacc gctgggcaag
      361 aggactacgc agccattcga gataactact ttcggagtgg ggaagggttt cttcttgtgt
      421 tctcaatcac agaacatgaa tcctttacag caactgccga attcagggaa cagattctcc
      481 gtgtgaaggc tgaagaagat aaaattccac tgctcgtcgt gggaaacaag tctgacctag
      541 aggagcggag gcaggtgcct gtggaggagg ccaggagtaa agccgaagag tggggcgtgc
      601 agtacgtgga gacgtcagcg aagacccggg ccaacgtgga caaggtgttc tttgacctaa
      661 tgagagaaat cagaacaaag aagatgtcag aaaacaaaga caagaatggc aagaaaagca
      721 gcaagaacaa gaaaagtttt aaagaaagat gttgcttact atgaaaccca gctttcttgt
      781 acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
      841 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      901 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
      961 cttgtataaa tcctgg
//
