LOCUS       sequence-205660-        8498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing VOPP1, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) VOPP1
FEATURES             Location/Qualifiers
     source          1..8498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    901..922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(937..942,943..960,961..967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     980..1002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     999..1015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     999..1015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1034..1054
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1036..1054
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1082..1308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1309..1489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1536..1661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2154..2387
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2572..2616
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2883..2999
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3022..3325
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3326..3529
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3480..3500
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3526..3550
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3634..3658
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4184..4208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4239..4827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4292..4312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4710..4721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4841..5340
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4876..4899)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5273..5292
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5361..5960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5361..5380)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5857..5877
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6098..6331
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6403..6524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6440..6459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6494..6513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6564..6699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6719..6738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6720..6738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6748..6765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6748..6764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6757..6779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6906..7361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6993..7012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7203..7224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7387..7491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7492..7560,7561..8352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7710..7729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      901 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      961 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1021 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     1081 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     1141 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     1201 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     1261 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1501 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1561 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1621 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1681 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1741 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1801 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1861 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1921 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1981 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2041 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2101 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2161 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2221 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2281 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2341 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2401 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2461 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2521 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2581 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2641 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2701 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2761 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2821 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     2881 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2941 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     3001 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3061 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3121 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3181 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3241 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3301 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3361 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3421 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3481 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     3541 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
     3601 ctagtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggcttc
     3661 accatgaggc gccagcctgc gaaggtggcg gcgctgctgc tcgggctgct cttggagtgc
     3721 acagaagcca aaaagcattg ctggtatttc gaaggactct atccaaccta ttatatatgc
     3781 cgctcctacg aggactgctg tggctccagg tgctgtgtgc gggccctctc catacagagg
     3841 ctgtggtact tctggttcct tctgatgatg ggcgtgcttt tctgctgcgg agccggcttc
     3901 ttcatccgga ggcgcatgta ccccccgccg ctgatcgagg agccagcctt caatgtgtcc
     3961 tacaccaggc agcccccaaa tcccggccca ggagcccagc agccggggcc gccctattac
     4021 accgacccag gaggaccggg gatgaaccct gtcgggaatt ccatggcaat ggctttccag
     4081 gtcccaccca actcacccca ggggagtgtg gcctgcccgc cccctccagc ctactgcaac
     4141 acgcctccgc ccccgtacga acaggtagtg aaggccaagt agaacccagc tttcttgtac
     4201 aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     4261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4621 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4741 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4801 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc
     4861 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     4921 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     4981 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     5041 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     5101 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     5161 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     5221 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     5281 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     5341 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5401 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5461 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5521 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5581 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5641 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5701 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5761 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5821 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5881 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5941 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     6001 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
     6061 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
     6121 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6181 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6241 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6301 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6361 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     6421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     6541 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6601 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6661 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     6721 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6781 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6841 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6901 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6961 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7021 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7321 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     7381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7441 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7501 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7561 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7621 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7681 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7741 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7801 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7861 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7921 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7981 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8041 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8101 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8161 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8221 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8281 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8341 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8401 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8461 ccttaacgtg agttttcgtt ccactgagcg tcagaccc
//
