LOCUS       sequence-205676-       11487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation L406I with approximately
            wildtype activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTL406I-myc
FEATURES             Location/Qualifiers
     source          1..11487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(700..718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        786..890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(891..959,960..1751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1109..1128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1922..2510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2411..2430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2941..3086
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3129..4166
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4164..4183)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4208..4256
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4910..5289
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5290..5493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5444..5464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5490..5514
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5542..5551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6217..6246
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7535..7669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7559..7578)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7613..7632
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7812..7834)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8394..10183
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
       61 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
      121 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
      181 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
      241 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
      301 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
      361 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
      421 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
      481 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
      541 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
      601 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
      661 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      901 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      961 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1021 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1081 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     1141 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1201 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1261 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1321 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1381 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     1441 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1501 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1561 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1621 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1681 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1741 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1801 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2041 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2521 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
     2581 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     2641 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     2701 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     2761 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     2821 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
     2881 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
     2941 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
     3001 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     3061 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
     3121 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     3181 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     3241 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     3301 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     3361 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     3421 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     3481 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     3541 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     3601 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     3661 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     3721 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     3781 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     3841 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     3901 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     3961 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4021 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4081 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     4141 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4201 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     4261 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4321 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4381 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
     4441 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     4501 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     4561 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     4621 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
     4681 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     4741 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     4801 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     4861 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     4921 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4981 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5041 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5101 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5161 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5221 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5281 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     5341 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5401 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     5461 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
     5521 agctgggtac cagctgctag cgccaccatg gagaccaccc ccctgaacag tcaaaagcaa
     5581 ctctctgcct gtgaggatgg agaggactgt caggagaatg gcgttcttca aaaggttgta
     5641 cccacccctg gagataaggt ggaaagcggc cagatttcca acggctacag cgccgtgcca
     5701 tcacctggag ccggggatga tacacggcac agcattcccg caaccaccac gacactggtt
     5761 gcagagcttc accagggcga gagggagacg tggggcaaaa aggtagattt tctgctttcc
     5821 gtgatcggtt acgcggtcga tctggggaac gtgtggaggt tcccttacat ttgttaccaa
     5881 aacggagggg gtgccttctt gctgccctac acaattatgg cgatttttgg gggtatcccg
     5941 ctcttctaca tggaactggc cttgggtcag taccaccgga acggctgcat tagcatttgg
     6001 cgcaagatat gccccatttt caagggaatc ggatacgcaa tctgtataat cgcgttctat
     6061 attgcatcat attacaacac catcatggcc tgggcccttt attacttgat cagcagtttc
     6121 accgaccaat tgccgtggac gagctgtaag aactcatgga ataccgggaa ctgtaccaac
     6181 tacttctctg aggacagctc aggaggtagc gggggtgaac agaagctgat tagcgaggag
     6241 gacttgggca gtccaggcgg aagcggcaac atcacttgga cactccatag tacgagcccc
     6301 gcagaggagt tttacaccag gcacgtactg cagattcata ggagcaaggg tctgcaagac
     6361 ctgggcggta ttagctggca gctcgcattg tgcataatgt tgatctttac ggtgatctat
     6421 ttctctatct ggaagggagt aaagacatcc ggcaaggtgg tgtgggtgac ggcgaccttc
     6481 ccctacatta tactcagcgt gttgttggtg aggggggcaa ccctgcctgg ggcctggcga
     6541 ggcgtgctgt tctatctcaa gccaaactgg cagaagcttc ttgaaaccgg agtgtggatc
     6601 gacgcggccg cgcaaatctt cttttccctc ggtcccggct tcggggtcct tctggcattc
     6661 gcaagctaca acaagttcaa caataactgc taccaggacg ccctcgtgac aagtgtagtg
     6721 aactgcatga cctctttcgt gagcgggttt gtgatcttca cagtactcgg atatatggct
     6781 gagatgagga acgaagatgt tagtgaggtt gccaaagacg ctggcccgag cctgatcttc
     6841 atcacatacg ccgaggctat cgcgaacatg cccgcatcta ctttcttcgc catcatcttt
     6901 ttcctgatgc tgataaccct tgggctggat agcaccttcg ccggactgga gggtgtcatc
     6961 actgccgttc tcgacgagtt cccccatgtg tgggccaagc gacgggaaag gttcgtgctg
     7021 gccgtcgtaa tcacatgctt cttcggctca ctggtgaccc ttacctttgg cggtgcgtac
     7081 gtggtcaagc tgctcgaaga gtacgccact ggaccggcgg ttctgaccgt ggcattgatc
     7141 gaggccgtgg ccgtaagctg gttttacggc atcacgcagt tctgtaggga cgtgaaagag
     7201 atgctgggct tttctcccgg ttggttttgg aggatttgtt gggtagcaat tagccccctg
     7261 tttctccttt tcattatttg ctcttttctg atgtctccac cgcagctccg actgtttcag
     7321 tacaactacc cctactggtc aattatcctg ggctattgta tcggcacctc aagtttcatc
     7381 tgcatcccca catacatcgc atacagactg ataattacgc ccggtacttt taaggagcga
     7441 atcattaaaa gcataacccc cgaaaccccc accgagatcc cttgtggcga tataagactc
     7501 aatgcggtct aatagctcga ggccggcaag gccggatcca gacatgataa gatacattga
     7561 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7621 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     7681 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     7741 aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat
     7801 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7861 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7921 gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta
     7981 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     8041 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     8101 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     8161 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     8221 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     8281 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     8341 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     8401 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     8461 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     8521 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     8581 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     8641 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     8701 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     8761 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     8821 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     8881 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     8941 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     9001 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     9061 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     9121 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     9181 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     9241 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     9301 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     9361 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     9421 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     9481 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     9541 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     9601 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     9661 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     9721 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     9781 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     9841 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     9901 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     9961 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
    10021 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat
    10081 acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca
    10141 cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct
    10201 taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa
    10261 tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac
    10321 atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca
    10381 gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat
    10441 acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc
    10501 tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca
    10561 gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac
    10621 acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca
    10681 tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg
    10741 gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga
    10801 ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa
    10861 agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta
    10921 gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat
    10981 ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg
    11041 cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg
    11101 ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg
    11161 tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc
    11221 ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct
    11281 ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt
    11341 cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc
    11401 cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct
    11461 gcccctcctc ctgctcctgc ccctcct
//
