LOCUS       sequence-205696-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing CRIP2, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) CRIP2
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(607..631)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(715..735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcgacaagac cgtatatttt gcggaaaagg tctctagtct gggtaaagat tggcacaaat
       61 tctgcctcaa gtgcgaacgt tgctcaaaga ccctgacacc tggtggccat gctgagcacg
      121 atggtaaacc cttttgtcat aagccttgtt atgcaaccct ttttggcccc aagggggtga
      181 acattggtgg ggccgggtcc tacatatacg agaagccatt ggccgaggga ccgcaagtta
      241 ccgggcccat tgaggtcccc gcagcccggg cagaggaacg gaaggccagc gggccgccta
      301 agggcccatc tcgcgcctcc tcagtgacca cattcactgg ggaacctaat acatgtcctc
      361 gatgtagtaa aaaggtttac ttcgccgaaa aagtcacgag cttaggaaag gattggcata
      421 gaccttgcct aaggtgtgag aggtgcggaa aaacactcac tcccggagga cacgctgagc
      481 acgacggcca gccatactgc cacaagccct gttacggcat cctgttcggc cccaaaggag
      541 tgaacactgg cgctgtgggc agctatatct atgacagaga cccagagggc aaagtgcagc
      601 catgaaaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
      661 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atga
//
