LOCUS       sequence-205708-        4221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains the VGF promoter (about 700 bp) plus 800 bp of 5' 
            untranslated sequence. The HindIII (5')-HincII(3') fragment acts as 
            a NGF-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Possenti R, Di Rocco G, Nasi S, Levi A
  TITLE     Regulatory elements in the promoter region of vgf, a nerve growth 
            factor-inducible gene.
  JOURNAL   Proc Natl Acad Sci U S A. 1992 May 1;89(9):3815-9.
  PUBMED    1570299
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1992-05-1"; volume: "89(9)"; pages: 
            "3815-9"
COMMENT     Sequence Label: pXT
FEATURES             Location/Qualifiers
     source          1..4221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     516..535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     769..786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    903..924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(939..944,945..962,963..969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    977..993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     982..1004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1001..1017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1001..1017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2622..2639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2622..2638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2631..2653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2847..2866)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2966..2988
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3026..3044)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3112..3216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3217..3285,3286..4077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3435..3454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      781 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      841 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      901 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      961 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1021 gattacgcca agcttaaggg tgtgggagag ttggattaaa agggggcaca acagggaccc
     1081 cttatcaacc acagcaaagt ccgctggcca gaagggcaga cacacctttt ttccccaccc
     1141 ccttcagaac tggtttgaac agacaggacc cagaggcaca cgtatcgtca atctttccct
     1201 tttctcttcc gactaggacc ctttccaagg tgattaccca aaccgcaaga tccacccatc
     1261 tgcgctgacc ctgggcgggc atcccacacc gcagatccac cccctcttcc tctcaggttc
     1321 aagtcgtcct tgggcgtgct ccctatagac catactgtgg cggagtaggg aggcaggccc
     1381 cttccccggc tttcaatccc ttaccgtccc cccatccagt actgatcttt tactggcgcc
     1441 caattagatg ctggcggtgc cctcttccta ttctgctcag caacgcgggc tccgcgcctc
     1501 aagagctttg taacccaccc tcccgccttc tggctcctct gtacccaccc ttccattcat
     1561 ttttccattc attcattcat ccttttctcc tcgtccctcc ttcattcatt catagccccc
     1621 tgccccgccc gcctagacat ttcattcatt cattcattca ttcatttccc ggagctccgc
     1681 tagctcacgc ccctctagcc gagggcccta ctgcgcaggt gcaggccggg agcgacgctt
     1741 atcctccaat cattggactt ccacagtcca ccaggcaccc gcccgcttcc ccatgaatga
     1801 acattgacgt caatggggcg gggcgagacc acgtgacccc gcgcgctccc ctttataaag
     1861 cagcggtggc gcgggcgctg tccagcgtgc tgaagccgga gcgagagcgc tgttgctgac
     1921 ccagctgagc ccagctccta ggacgccagc cctcgaccat ctttcatact ccagccacgg
     1981 aacggagcca gggcagacgg gtccggattt tccccctgcc ccgaccctcc tctccacctc
     2041 ccgccgtcgt gacaccggct ggtaaatact ccgctgtcca tccctctaac cctcttcact
     2101 cgtgggcgtg aaaggaggat tttctttcct tatggggtat tgagagcata gcggtgtgcc
     2161 cccgggaagg gtctttggta aacggggagt ctctgcttct ctgtcgctga tgaagtcgcc
     2221 cacgcaccat ccggggagtc ctgtggggag ggaacagatc ccccctccca cttatcattg
     2281 ctgcttagta cacgggcgct ggctgagaga tgactttggg aagggggcga caggaggcac
     2341 tgggtaccca gaacgaggat tgcgagcgtt ctctgaccat ttgcacgacc ccagagattg
     2401 ccacatcttt cttgttctct gctaaacgtt tctcttcggg taaggttaga gaaatgaggg
     2461 aaaccccgga tttcctgctt aaaggtgggg ggagggaatg cagaaataga aagagggaaa
     2521 ttcttataga aatcctctat ttctctggtt tcttctgttt ttcagtctct ggcagcccgt
     2581 tggtcaactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa
     2641 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2701 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2761 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2821 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2881 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2941 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3001 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3061 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3241 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3301 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3361 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3421 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3481 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3541 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3601 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3661 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3721 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3781 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3841 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3901 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3961 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4021 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4081 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4201 tcgttccact gagcgtcaga c
//
