LOCUS       sequence-205783-        8599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc 
            using the KpnI and NotI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Braun JE, Friedman LJ, Gelles J, Moore MJ
  TITLE     Synergistic assembly of human pre-spliceosomes across introns and 
            exons.
  JOURNAL   Elife. 2018 Jun 22;7. pii: 37751. doi: 10.7554/eLife.37751.
  PUBMED    29932423
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-06-22"; volume: "7. pii: 37751. doi"; pages: " 
            10.7554/eLife.37751"
COMMENT     Sequence Label: pcDNA5-FRT-TetO-Snu114-fSNAPc
FEATURES             Location/Qualifiers
     source          1..8599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3965..4510
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(4551..4568)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4557..4781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4914..4933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5065..5112
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5120..6142
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5120..6142
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6272..6393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6309..6328)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6363..6382
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6442..6458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6442..6458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6455..6477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6466..6482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6490..6496,6497..6514,6515..6520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6535..6556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6673..6690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6844..7432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6924..6943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7603..8394,8395..8463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8226..8245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8464..8568)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctgccaccat ggacaagctt tccagcggta ccatggatac
     1021 cgacttatat gatgagtttg ggaattatat tggaccagag cttgattctg atgaagatga
     1081 tgatgaattg ggtagagaga ccaaagatct tgatgagatg gatgatgatg acgacgacga
     1141 tgacgtagga gatcatgacg atgaccaccc tgggatggag gtggtgctgc atgaggacaa
     1201 gaagtactac ccaacagccg aggaggtgta tggtcctgag gtggagacca tagttcaaga
     1261 ggaagacact cagcctctca cagaacccat tattaagcca gtgaaaacca agaaattcac
     1321 tctgatggag cagacattac ctgttacggt gtatgagatg gatttcttgg cggatctgat
     1381 ggataactca gagctcatca gaaatgtgac cctttgtgga catctccacc atggcaagac
     1441 atgttttgtg gattgtttaa ttgaacagac tcacccggaa atcagaaagc gctatgacca
     1501 agatctgtgc tatactgaca tcctcttcac agagcaagag agaggtgtag gcatcaaaag
     1561 cactcctgtg acagtggtct tgccagacac caaaggaaaa tcttatctct tcaatatcat
     1621 ggacactcca ggacatgtga atttctctga tgaggtcaca gctggcttgc gcatctcaga
     1681 tggagtggtc cttttcattg atgctgctga gggggtgatg ctgaacacag agcggctgat
     1741 caagcatgcg gtgcaggaga ggctggcagt cactgtgtgc atcaacaaga ttgaccggct
     1801 gatcctggag ctgaagctgc ctccaactga tgcttattac aagctgcgcc acattgtgga
     1861 tgaggtcaat ggattaataa gcatgtattc cactgatgag aacctgatcc tttccccact
     1921 cctgggtaac gtctgcttct ccagctccca gtacagcatc tgcttcacgc tgggctcctt
     1981 tgccaagatc tatgccgaca cctttggtga cattaattac caagaatttg ctaaaagact
     2041 ctggggtgac atctacttca accctaagac gcgaaagttc accaaaaagg ccccaactag
     2101 cagctcccag agaagtttcg tggagtttat cttggagcct ctttataaga tcctcgccca
     2161 ggttgtaggt gacgtggaca ccagcctccc acggacccta gacgagcttg gcatccacct
     2221 gacgaaggag gagctgaagc tgaacatccg ccccttgctc aggctggtct gcaaaaagtt
     2281 ctttggcgag ttcacaggct ttgtggacat gtgtgtgcag catatccctt ctccaaaggt
     2341 gggcgccaag cccaagattg agcacaccta caccggtggt gtggactccg acctcggcga
     2401 ggctatgagt gactgtgacc ctgatggccc cctgatgtgc cacactacta agatgtacag
     2461 cacagatgat ggagtccagt ttcacgcctt tggccgggtg ctgagtggca ccattcatgc
     2521 tgggcagcct gtgaaggtac tgggggagaa ctacaccctg gaggatgagg aagactccca
     2581 gatatgcacc gtgggccgcc tttggatctc tgtggccaga taccacatcg aggtgaaccg
     2641 tgttcctgct ggcaactggg ttctgattga aggtgttgat caaccaattg tgaagacagc
     2701 aaccataacc gaaccccgag gcaatgagga ggctcagatt ttccgaccct tgaagttcaa
     2761 taccacatct gttatcaaga ttgctgtgga gccagtcaac ccctcagagc tgcccaagat
     2821 gcttgatggc ctgcgcaagg tcaacaagag ctatccatcc ctcaccacca aggtggagga
     2881 gtctggcgag catgtgatcc tgggcactgg ggagctctac ctggactgtg tgatgcatga
     2941 tttgcggaag atgtactcag agatagacat caaggtggct gacccagttg tcacgttttg
     3001 tgagacggtg gtggaaacat cctccctcaa gtgctttgct gaaacgccta ataagaagaa
     3061 caagatcacc atgattgctg agcctcttga gaagggcctg gcagaggaca tagagaatga
     3121 ggtggtccag attacgtgga acaggaagaa gctgggagag ttcttccaga ccaagtacga
     3181 ttgggatctg ctggctgccc gttccatctg ggcttttggc cctgatgcga ctggccccaa
     3241 cattctggtg gatgatactc tgccctctga ggtggacaag gctcttcttg gttcagtgaa
     3301 ggacagcatc gttcaaggtt tccagtgggg aaccagggag ggccccctct gtgatgaatt
     3361 gattcggaat gtcaagttta agatcctgga tgcggtggtt gcccaggagc ccctgcaccg
     3421 gggcgggggc cagatcatcc ccacagccag gagagtcgtc tactctgcct tcctcatggc
     3481 tactcctcgt ctgatggagc cttactactt tgtagaggtc caggcccctg cagattgcgt
     3541 ctctgcagtt tataccgtcc tggccaggcg cagggggcac gtgactcagg atgcacccat
     3601 cccaggctcc cctctgtaca ccatcaaagc ttttatcccg gccatcgact cttttggctt
     3661 tgagactgat ctccggactc acacccaggg acaagccttt tctctgtctg tcttccacca
     3721 ctggcagatt gtgcctggtg atcccctgga caagagcatt gtcatccgcc ccttggagcc
     3781 acagccagct cctcacctgg cccgggaatt catgatcaaa acccgccgta ggaagggcct
     3841 cagtgaagat gtgagcatca gcaaattctt cgatgatcct atgttgctgg aacttgccaa
     3901 acaggatgtt gtgctcaatt accccatgtg cggccgcggc tccggaggct ccggcgggag
     3961 cggcatggac aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct
     4021 ggaactgtct gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc
     4081 tgccgccgac gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact
     4141 gatgcaggcc accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt
     4201 ccctgtgcca gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct
     4261 gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggccgc
     4321 cctggccggc aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt
     4381 gcccattctg atcccctgcc accgggtggt gcagggcgac ctggacgtgg ggggctacga
     4441 gggcgggctc gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc
     4501 tgggctgggt tagctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4561 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4621 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4681 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4741 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4801 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5041 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     5101 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     5161 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     5221 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     5281 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     5341 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     5401 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     5461 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     5521 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     5581 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     5641 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     5701 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     5761 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     5821 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     5881 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     5941 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     6001 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     6061 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     6121 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     6181 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6241 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6301 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6361 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6421 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8581 aaaagtgcca cctgacgtc
//
