LOCUS       sequence-205832-        2802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC6
FEATURES             Location/Qualifiers
     source          1..2802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..72
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(610..627)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(741..759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        827..931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1153..1944
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2115..2703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2604..2623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
       61 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      121 ccgattcatt aatcactctg tggtctcaaa tggcgtcgcc gagcaaacgt cgagagatgg
      181 atatgatgaa actgatgatg agtgattaca aggtggatac tgtcaacgac gatttgcaaa
      241 tgttctatgt cacattccat ggacccactg acagtcttta tcaggggggt gtatggaaga
      301 taaaagtgga acttcccgaa gcttatccat acaaatctcc atctgttgga tttgtcaaca
      361 agatttatca ccctaatgtc gatgaatctt ctggtgctgt ttgcttagat gttataaacc
      421 agacttggag cccaatgttt gatcttataa atgtgttcga atccttcctt ccgcaactgc
      481 ttctatatcc aaacccatca gatccattta atggagaagc cgcttctctg ttgatgcgtg
      541 accgtgcagc ctatgagctc aaagtgaaag aatattgtga gaaatatgca aaaccagagg
      601 agattttaag tgatgatgat gatgatgata gcatgagcga agatggttcg gactctgatg
      661 atgatgacga cgacgaaatc gttgggaaag ccgatccatg agctttgaga ccacgaagtg
      721 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      781 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      901 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
      961 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1021 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1081 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1141 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1201 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1261 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1321 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1381 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1441 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1501 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1561 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1621 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1681 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1741 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1801 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     1861 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     1921 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     1981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2761 atgttctttc ctgcgttatc ccctgattct gtggataacc gt
//
