LOCUS       sequence-205855-        8962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of 
            P39880), with an N-terminal Myc tag and a C-terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Wilson BJ, Harada R, LeDuy L, Hollenberg MD, Nepveu A
  TITLE     CUX1 transcription factor is a downstream effector of the 
            proteinase-activated receptor 2 (PAR2).
  JOURNAL   J Biol Chem. 2009 Jan 2;284(1):36-45. doi: 10.1074/jbc.M803808200. 
            Epub 2008 Oct 24.
  PUBMED    18952606
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M803808200"; journalName: "J Biol Chem"; date: 
            "2009-01-2- 2"; volume: "284"; issue: "1"; pages: "36-45"
COMMENT     Sequence Label: pXJ42-p200 CUX1
FEATURES             Location/Qualifiers
     source          1..8962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        345..548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     499..519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     545..569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          803..1375
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1383..1402
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1430..1449
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1430..1448
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1482..1511
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6027..6053
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6087..6208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6124..6143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6178..6197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6242..6259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6413..7001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6493..6512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7172..7963,7964..8032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7795..7814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8033..8137)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8205..8223
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8261..8283)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8383..8402
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8469..8924)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8606..8627)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8818..8837
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctagagtc cgttacataa cttacggtaa
       61 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      301 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      361 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      421 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      481 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      541 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      601 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgg
      661 acccctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
      721 gacycyksrk yskkswwysk tkywykskaw ygkammcyta ggcttttgca aaaagctccg
      781 gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
      841 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
      901 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
      961 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     1021 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     1081 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     1141 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     1201 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     1261 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     1321 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1381 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt aatacgactc
     1441 actatagggc gaattagatc tggtaccctc gagccaccat ggagcagaag ctcatctctg
     1501 aagaggacct catgttgtgc gtacgcggag ccaggttgaa gagagaactc gatgccaccg
     1561 caacggtatt ggcgaaccgg caggatgaga gtgagcagtc cagaaagcgg cttatcgaac
     1621 agagccggga gttcaagaag aacactccag aggatttgcg caagcaggta gcgccgctgc
     1681 tgaagagttt ccaaggagag attgatgcac tgagtaaaag aagcaaggaa gctgaagcag
     1741 ctttcttgaa tgtctacaaa agattgattg acgtcccaga tcccgtacca gctttggatc
     1801 tcggacagca actccagctc aaagtgcagc gcctgcacga tattgaaaca gagaaccaga
     1861 aacttaggga aactctggaa gaatacaaca aggaatttgc tgaagtgaaa aatcaagagg
     1921 ttacgataaa agcacttaaa gagaaaatcc gagaatatga acagacactg aagaaccaag
     1981 ccgaaaccat agctcttgag aaggaacaga agttacagaa tgactttgca gaaaaggaga
     2041 gaaagctgca ggagacacag atgtccacca cctcaaagct ggaggaagct gagcataagg
     2101 ttcagagcct acaaacagcc ctggaaaaaa ctcgaacaga attatttgac ctgaaaacca
     2161 aatacgatga agaaactact gcaaaggccg acgagattga aatgatcatg acggaccttg
     2221 aaagggcaaa ccagagggca gaggtggctc agagagaggc ggagacctta agggaacagc
     2281 tctcatcggc caatcactcc ctccagctgg cctcacagat ccagaaggca ccagacgtgg
     2341 agcaggccat agaggtgctg acccgctcca gcctagaagt tgagttggcc gccaaggagc
     2401 gggagatcgc acagctggtg gaggacgtgc agagactcca ggccagcctc accaagctgc
     2461 gggagaattc ggccagccag atctcacagc ttgagcagca gctgagcgcc aaaaacagca
     2521 cactcaaaca actggaagaa aaactcaaag gccaggctga ctatgaagag gtgaaggaag
     2581 agctgaacat tctgaagtcc atggagtttg caccgtccga gggcgctggg acacaggatg
     2641 cggccaagcc cctggaggtg ctgttgctgg agaagaaccg ctcgctgcag tccgagaacg
     2701 ccgcgctgcg catctccaac agcgacctga gcgggtcagc caggaggaaa gggaaagacc
     2761 agcctgaaag tcggcgcccg ggatctttgc cggccccccc tccttctcag ttgccccgca
     2821 acccggggga gcaggcttcc aatactaatg gtacacacca gttctcacca gcggggttaa
     2881 gtcaagactt tttcagctca tccctggcaa gccccagcct acccctggct tctacaggaa
     2941 aatttgcact aaactctctt ctccagcggc agctaatgca gtccttctac tccaaggcta
     3001 tgcaggaagc cggaagcaca agcatgattt tttcaacagg tccatacagc acaaactcca
     3061 tatcttccca aagtccatta caacaaagcc cagatgtcaa tggcatggcc ccatccccca
     3121 gccagtcaga aagtgctggg agcgtctccg agggcgagga gatggacact gcagaaatcg
     3181 cccggcaggt caaagagcag ctgattaagc acaatatcgg acaacgtatt ttcggacatt
     3241 atgtgttggg actgtctcaa gggtccgtga gcgagattct ggcccggccc aagccatgga
     3301 ataaactgac tgttcgtggc aaggagccat ttcacaagat gaaacagttc ctctccgatg
     3361 agcagaacat cctggccctc cgtagcatcc aaggcagaca aggagagaat ccaggccaga
     3421 gcctgaacag actatttcag gaagtaccga aacgaagaaa tgggtctgaa ggtaacatca
     3481 ccacccggat ccgagcctcg gagactggct ctgatgaagc catcaagtcc atcctagagc
     3541 aagccaagag ggagctccaa gtgcagaaaa ctgcagagcc ggcccagcct tcctccgcat
     3601 ccggcagcgg gaactctgat gacgccatcc gctccatcct gcagcaagcc cgccgggaga
     3661 tggaggccca gcaggctgcc ctcgaccctg ccttaaagca ggcaccactg tcccagagtg
     3721 acatcaccat cctcaccccc aagcttctgt ccacctcgcc catgcccacc gtgtccagct
     3781 acccacctct cgccatctcc ctgaagaagc cctccgcagc tcctgaggcc ggtgcctctg
     3841 ctctgccgaa ccccccggcc ctcaaaaagg aggcccagga cgcccccggg ctggaccccc
     3901 agggagcagc cgattgtgca caaggggtcc tgagacaggt gaaaaatgag gtgggccgca
     3961 gcggtgcctg gaaggaccac tggtggagcg cggtgcagcc ggagagaaga aatgccgcct
     4021 cctccgagga ggccaaggcc gaagaaacgg gcggcgggaa agagaagggc agcggtggca
     4081 gcggaggtgg cagccagcct cgggccgagc gcagtcagct ccagggaccc tcgtcgtcag
     4141 agtactggaa ggagtggccc agcgctgagt ccccatactc ccagagctca gagctgagtc
     4201 tgaccggggc cagccgcagc gagacaccac agaacagccc cctgccatcc tccccgatcg
     4261 tgcccatgtc caagcccacc aagccctcgg tccccccgct gacccccgag cagtacgagg
     4321 tctacatgta ccaggaggtg gacaccatcg agctcacccg gcaggttaag gaaaagctgg
     4381 ccaagaacgg catctgccag agaatcttcg gggagaaggt gctgggcctg tcccagggca
     4441 gcgtcagcga catgctgtcc cgaccgaagc catggagcaa gctgacgcag aaaggccgag
     4501 aacccttcat ccggatgcag ctctggctga acggcgagct aggccagggt gttctacccg
     4561 tccagggcca gcagcaaggg ccagtcctcc actccgtgac atcgctccag gacccgctgc
     4621 agcagggctg tgtgagctca gaaagcactc caaagacctc cgccagctgc agccctgccc
     4681 ctgagtcccc gatgagttcc agtgagtcgg tgaagagcct gaccgagctg gtccagcagc
     4741 cctgtccccc catcgaggcg agcaaggaca gcaagccacc agagcccagt gacccgccag
     4801 catccgactc ccagcccaca accccgctgc ctctctccgg acactcggcc ctcagcatcc
     4861 aagaattagt agccatgtcc ccggagctgg acacctacgg cataaccaag cgggtgaagg
     4921 aggtgctgac ggacaacaac ctcggccagc gcttatttgg ggagaccatc ttagggctca
     4981 cccaaggctc tgtctctgac ctccttgccc gccccaaacc ctggcataag ctcagtctga
     5041 aaggacgaga gcccttcgtc cggatgcagc tgtggctgaa cgaccccaac aatgtggaga
     5101 agctgatgga catgaaacgg atggagaaga aagcctacat gaagcggcgg cacagctcag
     5161 tcagtgacag ccagccctgc gaaccgccct ctgtcggcac cgagtacagc cagggcgcca
     5221 gcccccagcc ccagcaccag ctgaagaaac cccgggtggt gctggctccg gaggagaagg
     5281 aggcgctgaa acgagcgtat cagcaaaagc catacccgtc accaaaaacc atcgaagacc
     5341 tcgccaccca gctcaacctg aaaaccagca ccgtcatcaa ctggttccac aactacaggt
     5401 ctcggatccg cagagaactg ttcattgagg aaattcaggc cgggagtcag ggccaggcgg
     5461 gcgccagcga ctcaccctcg gcccgcagcg gccgggcggc gcccagctcg gagggcgaca
     5521 gctgcgacgg cgtggaggcc actgagggcc caggcagcgc cgacaccgag gagcccaagt
     5581 ctcagggaga ggccgagcgg gaggaggtgc cgcggccggc ggagcagacg gagccgccgc
     5641 cctcggggac cccgggcccg gacgacgccc gcgacgacga ccacgaggga ggccccgtgg
     5701 aaggcccggg gcccctgccc agccccgcct cckckaccsm taccgscgcg cccgnngccc
     5761 ccgaggacgc cgcyaccwmw gyskmcgmcg sgccggggga gggccccgcg gccccgagct
     5821 ccgcgccgcc gcccagcaac agcagcagca gcagcgcccc ccgcaggccc agctcgctgc
     5881 agagcctttt cggcctcccc gaggccgcgg gcgcccggga ctcgcgcgac aaccccctgc
     5941 gcaagaagaa ggccgcgaac ttgaacagca tcatccaccg cctggagaag gccgccagcc
     6001 gggaggaacc tatcgaatgg gagttctatc catacgacgt acctgactac gcatgagcgg
     6061 ccgcgaattc gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     6121 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6181 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagac tcttccgctt
     6241 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6301 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6421 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6481 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6541 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6601 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6661 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6721 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6781 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6841 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6901 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6961 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7021 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7081 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7141 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7201 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7261 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7321 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7381 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7441 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7501 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7561 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7621 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7681 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7741 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7801 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7861 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7921 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7981 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8041 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8101 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8161 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     8221 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8281 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8341 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     8401 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8461 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8521 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     8581 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     8641 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     8701 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     8761 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     8821 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     8881 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     8941 ctgcgcaact gttgggaagg gc
//
