LOCUS       sequence-206130-        2736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC18
FEATURES             Location/Qualifiers
     source          1..2736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..55
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(658..676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        744..848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1070..1861
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2032..2620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2521..2540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
       61 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
      121 ctgtggtctc aaatgacaag ctcttcatct ccttcacgca aggcgttaag caagatcgcg
      181 tgtaataggt tacaaaagga gttatcagag tggcaagtaa atccaccgac tgggtttaag
      241 cacagagtta ctgataatct tcaaaaatgg gtaattgagg taacaggagc acctggtacg
      301 ctttacgcta atgaaactta taatcttcag gttgagtttc ctcaacatta tcctatggaa
      361 gctcctcagg tgatttttgt tccaccagca ccgttacatc ctcatattta cagcaatgga
      421 catatttgtt tagatattct gtatgactca tggtcaccag caatgacagt gagttcagtc
      481 tgcatcagca tcctctcaat gctatcaagt tcccccgaaa agcaacgccc gactgataac
      541 gatcgatatg tgaagaactg caagaacggg aggtcaccta aagagacaag gtggtggttc
      601 catgacgaca aggtttaagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc
      661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      841 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      901 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
      961 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1021 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1081 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1141 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1201 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1261 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1321 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1381 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1441 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1501 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1561 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1621 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1681 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1741 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1801 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     1861 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1921 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1981 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2041 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2101 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2161 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2221 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2281 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2401 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2641 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2701 tgattctgtg gataaccgta ttaccgcctt tgagtg
//
