LOCUS       sequence-206136-        4974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC24
FEATURES             Location/Qualifiers
     source          1..4974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..69
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2910..2928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2996..3100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3322..4113
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4773..4792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
       61 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      121 attcattaat cactctgtgg tctcaaatgg aaatgtccct tactgactct gattgggata
      181 gctccagcga cagtggtagc agtgaacacg aagaagtcga gttttcttat ggtggacggg
      241 cacagaacat tttctcaaac cttgaagaaa ccattggcaa aatcgatgag ttcctgtcgt
      301 tcgagagggg atttatgtat ggtgacattg tgcggtccgc cactgaacca tcaggacaga
      361 gtggcagggt tatcaacata gacatgtttg tcaatctcga aagtactcat gggaagatca
      421 tgaaggaagt tgataccaag aggcttcaaa agttgcgttc tatttcactc tctgattatg
      481 tgattaacgg accttgggtt ggaagggttg acaaaatagt tgagcgtgtc tctgtcaccc
      541 ttgatgatgg gaccaactat gaggtccttg tagatggtca agataaactt gtggccattc
      601 ccccaaattt actcgaggat tctcaatatt cgtattaccc agggcaaaga gttcaggtaa
      661 agctggccca tgcccccaga tcaactacat ggttatgcgg gacctggaga ggaacccagg
      721 ttatgggaac tgtttgcact gtagaagcag gacttgtcta cgtcgattgg gttgcctcca
      781 tcgtaatgga gggtgatcgg aatttaactg cacctcaagc tttgcagaat cctgagagtt
      841 taactttgtt accttgtgtt tctcatgcga gttggcagct tggtgactgg tgtatactcc
      901 ctggctcttc ccactgtgat atagcagagc ggcaaactcc aaatgtggct gcctacaatc
      961 tcaatgaatg ccataagaca ttccaaaaag ggtttaacag aaatatgcag aactcaggtt
     1021 tggatgagct atttgttatc acaaagacaa agatgaaggt tgctgttatg tggcaagatg
     1081 gtagctgcag tctgggagtt gattcccaac agctgcttcc tgttggtgct gttaatgctc
     1141 atgatttttg gcccgaacag tttgttgtgg aaaaggaaac ctgcaacagc aaaaaatggg
     1201 gagttgtgaa ggctgtcaat gctaaggaac aaactgtgaa ggtacaatgg acaatacagg
     1261 ttgagaaaga agcaacaggt tgtgttgatg aggtgatgga agaaattgtc agtgcatatg
     1321 aactgcttga gcaccctgat tttggattct gtttcagcga tgtggtggtc aagttacttc
     1381 cagaaggaaa atttgatcca aatgcagaca caatcgtcgc tacagaggcg aaacacctac
     1441 ttacagagag tgactacagt ggcgcatatt ttttgtcaag tattggtgtt gttacaggtt
     1501 ttaaaaatgg ttccgtgaag gtgaaatggg ccaatggttc tactagcaag gttgcaccat
     1561 gtgaaatttg gaaaatggaa aggtctgaat attccaactc tagcactgta agttcggaag
     1621 gcagtgttca agatctaagt cagaagatta gtcaatcgga tgaagcatct tcaaaccatc
     1681 aggaaacggg tctggtgaag ctctacagtg ttggtgaaag ttgcaacgag aacattccgg
     1741 aatgtagttc atttttcctt ccaaaagctg ccattggatt tatcacaaac cttgcatcaa
     1801 gcctttttgg ttatcagggt tccacttcgg ttataagttc acattcacgt tgcaatgatt
     1861 ctgaagatca aagtgactct gaggtccttg ttcaagaaac agcagaatca tatgacaact
     1921 ctgaaacaaa ttcaggtgaa gtggatatga ccaccacgat ggtcaacata cctatagaag
     1981 gaaaaggaat taacaagaca ctggattcaa ctcttctaga gaacagcagg aaccaagtga
     2041 gattcagaca gtttgatatg gttaatgact gctcggacca tcatttcctt tcttcggata
     2101 aagggttggc tcagtcccag gtcacaaaga gttgggtgaa gaaagtccag caagaatgga
     2161 gcaatttgga ggcaaatctt ccgaacacga tatatgtgcg tgtgtgtgaa gaaaggatgg
     2221 accttctgcg tgcagccctg gttggtgccc ctggaacgcc atatcacgat gggcttttct
     2281 ttttcgacat aatgcttcca cctcaatatc ctcatgagcc accaatggta cattatcatt
     2341 caggtgggat gcgactgaat ccgaacctgt atgagtcagg aagagtttgc ttgagtctgc
     2401 tgaatacatg gagtggctct ggcactgaag tatggaacgc agggagctcc tccatccttc
     2461 aacttcttct ttcgtttcag gctctggttc tgaatgagaa gccttacttc aatgaagctg
     2521 gctatgataa gcagttgggc cgagccgagg gagagaaaaa ctcagtgagt tacaatgaga
     2581 atgcattcct cataacctgc aaatccatga tctcaatgct ccgtaagcct ccaaagcatt
     2641 ttgagatgct tgtgaaggac cattttacgc accgggccca gcatgttctg gctgcgtgca
     2701 aggcttatat ggaaggcgtc cctgtaggat catcagctaa cctgcagggg aactcaacca
     2761 caaattccac cggtttcaag atcatgctct ccaaactcta cccaaaactt cttgaagcat
     2821 tctcagagat tggagttgat tgcgtccaag agattggacc agaatcataa gctttgagac
     2881 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2941 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3001 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3061 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     3121 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3181 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     3241 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     3301 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     3361 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     3421 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     3481 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     3541 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     3601 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     3661 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     3721 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     3781 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     3841 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     3901 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     3961 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     4021 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     4081 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tatt
//
