LOCUS       sequence-206199-        2964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC34
FEATURES             Location/Qualifiers
     source          1..2964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..76
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(907..925)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        993..1097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1319..2110
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2281..2869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2770..2789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
       61 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      121 ttggccgatt cattaatcac tctgtggtct caaatggcag aaaaggcctg tataaaacga
      181 cttcaaaagg aatatagagc actttgcaag gaaccagtct cccatgttgt tgctcgtcct
      241 tccccaaatg acattctcga gtggcattat gtgctggaag gcagtgaggg aacacctttt
      301 gcaggtggat tttactatgg aaagatcaag tttcctcccg agtatcctta caagccacca
      361 gggattacaa tgactacacc aaatggtcga ttcatgacac agaagaaaat ttgtttatct
      421 atgagtgatt ttcatccaga aagctggaat ccgatgtggt ctgtatcaag catacttaca
      481 ggacttctct catttatgat ggataccagt ccgacaactg gaagtgttaa cactactgta
      541 attgagaaac aacggttggc taagtcatct ctcgctttca attgtaaaac cccagcattt
      601 cgaaagctat ttccagagta tgtagagaag tacaaccagc agcaactagc tgagcaagct
      661 accacacaac tgacaacacc agagtctcct caaaagagcg ataccaaagt cgagtcagag
      721 aaaaccatcg atccaacaaa gggagattca gaaggtggct tgaaggagag gaaaaagaac
      781 aataaacagg gattgccagc gtggataata ctgttgctag tgtcggtttt cggtgtggta
      841 atggcgttgc ctctgcttca actgtgagct ttgagaccac gaagtggctc ttcagtggac
      901 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      961 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1021 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1081 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1141 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     1201 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1261 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     1321 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1381 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     1441 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     1501 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     1561 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1621 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1681 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1741 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1801 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1861 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1921 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1981 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2041 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     2101 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2161 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2881 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2941 gttatcccct gattctgtgg ataa
//
