LOCUS       sequence-206251-       11305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  virus-induced gene silencing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTRV2-GG
FEATURES             Location/Qualifiers
     source          1..11305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    182..206
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        674..1019
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     962..982
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        2689..2791
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2792..3475
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(2858..2877)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3795..4100
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3979..3998
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    4925..4949
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6280..6909
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7338..8411
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8477..8671
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8907..8929)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9015..9155
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9070..9089
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9170..9187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9341..9929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9421..9440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10175..10966)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atcatccgtg tttaaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
       61 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
      121 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
      181 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
      241 ggaattcaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      301 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      361 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      421 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      481 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      541 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      601 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      661 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      721 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      781 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      841 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      901 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      961 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
     1021 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
     1081 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1141 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1201 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1261 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1321 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1381 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1441 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1501 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1561 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1621 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1681 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1741 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1801 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1861 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1921 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1981 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     2041 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     2101 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2161 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2221 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2281 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2341 gtagcatttg agtttcgcaa tgcacgaatt acttaggaag tggcttgacg acactaatgt
     2401 gttattgtta gataatggtt tggtggtcaa ggtacgtagt agagtcccac atattcgcac
     2461 gtatgaagta attggaaagt tgtcagtttt tgataattca ctgggagatg atacgctgtt
     2521 tgagggaaaa gtagagaacg tatttgtttt tatgttcagg cggttcttgt gtgtcaacaa
     2581 agatggacat tgttactcaa ggaagcacga tgagctttat tattacggac gagtggactt
     2641 agattctgtg agtaaggtta ccgaattctt atgagagacc tgagacgttg atcggcacgt
     2701 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     2761 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     2821 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     2881 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     2941 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     3001 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     3061 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     3121 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     3181 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     3241 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     3301 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     3361 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     3421 gtactgcgat gagtggcagg gggggcgtaa acgccgcgtg gatccggctt actaaaagcc
     3481 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3541 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     3601 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     3661 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     3721 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     3781 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     3841 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     3901 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     3961 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtttccgtta
     4021 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4081 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtcg gtctcaggtg
     4141 ggcccgggca tgtcccgaag acattaaact acggttcttt aagtagatcc gtgtctgaag
     4201 ttttaggttc aatttaaacc tacgagattg acattctcga ctgatcttga ttgatcggta
     4261 agtcttttgt aatttaattt tctttttgat tttattttaa attgttatct gtttctgtgt
     4321 atagactgtt tgagatcggc gtttggccga ctcattgtct taccataggg gaacggactt
     4381 tgtttgtgtt gttattttat ttgtatttta ttaaaattct caacgatctg aaaaagcctc
     4441 gcggctaaga gattgttggg gggtgagtaa gtacttttaa agtgatgatg gttacaaagg
     4501 caaaaggggt aaaacccctc gcctacgtaa gcgttattac gcccgtctgt acttatatca
     4561 gtacactgac gagtccctaa aggacgaaac gggagaacgc tagccaccac caccaccacg
     4621 cttccatgct agagtcagca aaaatcacca gtctctctct acaaatctat ctctctctat
     4681 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     4741 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     4801 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcgacg
     4861 tcgagcttag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
     4921 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     4981 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     5041 cacagggttc ccctcgggat caaagtactt taaagtactt taaagtactt taaagtactt
     5101 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtgtt
     5161 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     5221 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     5281 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5341 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5401 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5461 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5521 gcacccgcga cctactggac attgccgaga gcatccagga ggccggcgcg ggcctgcgta
     5581 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5641 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5701 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5761 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5821 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5881 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5941 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     6001 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6061 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6121 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     6181 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     6241 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6301 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6361 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6421 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6481 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6541 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6601 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6661 gtcacggatg gaaggctaca agcggccttt gtagtgtcgc gggcgatcaa aggcacgcgc
     6721 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6781 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6841 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6901 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6961 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     7021 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7081 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7141 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     7201 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     7261 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     7321 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     7381 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     7441 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     7501 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     7561 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     7621 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     7681 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     7741 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     7801 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     7861 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     7921 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7981 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     8041 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     8101 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     8161 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     8221 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     8281 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     8341 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     8401 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     8461 gaaaaggtgt ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     8521 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     8581 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     8641 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     8701 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     8761 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     8821 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     8881 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8941 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9001 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     9061 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9121 cgcacagatg cgtaaggaga aaataccgca taaggcgctc ttccgcttcc tcgctcactg
     9181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9781 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatgat atatctccca
    10021 atttgtgtag ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt
    10081 ttggctgtga gcaattatgt gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa
    10141 gcggcgtcgg cttgaacgaa tttctagcta gacattattt gccgactacc ttggtgatct
    10201 cgcctttcac gtagtggaca aattcttcca actgatctgc gcacgaggcc aagcgatctt
    10261 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
    10321 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
    10381 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
    10441 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
    10501 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
    10561 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
    10621 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
    10681 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
    10741 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
    10801 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
    10861 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
    10921 cctctgatag ttgagtcgat acttcggcga tcaccgcttc ccccatgatg tttaactttg
    10981 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
    11041 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
    11101 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
    11161 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg
    11221 cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta
    11281 caatcagcat gctaccctcc gcgag
//
