LOCUS       sequence-20635-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(19..143)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(151..167)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(151..167)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(182..623)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(188..211)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    506..623
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
       61 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      121 tcagcttttt tgtacaaact tgtgatgggg gatccactag ttctagaatc cggggttttt
      181 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca
      241 tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttatgca
      301 gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
      361 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
      421 tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
      481 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
      541 ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca
      601 cccgctcggc ggcttctaat ccgtact
//
