LOCUS       sequence-206369-        3905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI067
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2230..2248)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2316..2420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2642..3433
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3604..3905,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagcc
      541 caagtagagg aatttcatag tgaattcaaa agattaactg tattccaccg tcgtattttg
      601 ataacattta gttattcctt ttcttttttt tcttctgcaa cagttttttt ttaatacatt
      661 tagtgttggt ttggttcaat gaaatattat atgttacttc tttttttgga aataaattat
      721 tcattctttc tactataaaa ggaattgttc atgctttttt gatacaatag tataccattt
      781 caaaagatac catagaccag ttattacatg aatcgccaaa acaacactaa aatcagaaaa
      841 tcagtatatt ttggtatagt ctccaacata caatcataaa acctctgtga aatttaaaat
      901 ctatatttga catttcaaag tttaacaaca tagttctaaa taattaccta aattttaagt
      961 caaatgtgaa ttatatttta ctcttcgata tcggttgttg acgattaacc atgcaaaaaa
     1021 gaaacattaa ttgcgaatgt aaataacaaa acatgtaact cttgtagata tacatgtatc
     1081 gacatttaaa cccgaatata tatgtatacc tataatttct ctgattttca cgctacctgc
     1141 cacgtacatg ggtgataggt ccaaactcac aagtaaaagt ttacgtacag tgaattcgtc
     1201 tttttgggta taaacgtaca tttaatttac acgtaagaaa ggattaccaa ttctttcatt
     1261 tatggtacca gacagagtta aggcaaacaa gagaaacata tagagttttg atatgttttc
     1321 ttggataaat attaaattga tgcaatattt agggatggac acaaggtaat atatgccttt
     1381 taaggtatat gtgctatatg aatcgtttcg catgggtact aaaattattt gtccttactt
     1441 tatataaaca aattccaaca aaatcaagtt tttgctaaaa ctagtttatt tgcgggttat
     1501 ttaattacct atcatattac ttgtaatatc attcgtatgt taacgggtaa accaaaccaa
     1561 accggatatt gaactattaa aaatcttgta aatttgacac aaactaatga atatctaaat
     1621 tatgttactg ctatgataac gaccattttt gtttttgaga accataatat aaattacagg
     1681 tacgtgacaa gtactaagta tttatatcca cctttagtca cagtaccaat attgcgccta
     1741 ccgggcaacg tgaacgtgat catcaaatca aagtagttac caaacgcttt gatctcgata
     1801 aaactaaaag ctgacacgtc ttgctgtttc ttaatttatt tctcttacaa cgacaatttt
     1861 gagaaatatg aaatttttat atcgaaaggg aacagtcctt atcatttgct cccatcactt
     1921 gcttttgtct agttacaact ggaaatcgaa gagaagtatt acaaaaacat ttttctcgtc
     1981 atttataaaa aaatgacaaa aaattaaata gagagcaaag caagagcgtt gggtgacgtt
     2041 ggcctcttca ttaactcctc tcatctaccc cttcctctgt tcgcctttat atccttcacc
     2101 ttccctctct catcttcatt aactcatctt caaaaatacc ctaatcacat tttgtaacaa
     2161 taatacaatt atacattaaa actctccgac aatgtgagac cacgaagtgg ctcttcagtg
     2221 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2281 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2341 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2401 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2461 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
     2521 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2581 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2641 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2701 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2761 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2821 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2881 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2941 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3001 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3061 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3121 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3181 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3241 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3301 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3361 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     3421 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3901 taagg
//
