LOCUS       sequence-20656-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(125..249)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             290..379
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(424..442)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(463..479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(463..479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(476..498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    487..503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(511..517,518..535,536..541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    556..577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gttatcgggg agaagtggct gatctcagcc caccgcgaaa atgacatcaa aaacgccatt
       61 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
      121 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
      181 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
      241 caaagtggtg atgggctgca ggaattcgat atcaagctta ggtggaatgt acccatacga
      301 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
      361 tgacgttcca gattacgctg ctcagtgcta actcgagggg gggcccggta ccagcttttg
      421 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
      481 gtgaaattgt tatccgctca caattccaca caacatacaa gccggaagca taaagtgtaa
      541 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      601 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      661 aggcggtttg cgtatt
//
