LOCUS       sequence-207066-        3363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-419
FEATURES             Location/Qualifiers
     source          1..3363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1337..1355)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1423..1527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1749..2540
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2711..3299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3200..3219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
       61 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc
      181 tcaaatgaac accggcggtg gccaatacac ggattttccg gcggtggaaa ctcacggagg
      241 acagttcata agttacgata tcttcggtag tttattcgag atcacatcta agtatcgtcc
      301 tccgattata ccaattggtc gtggagctta tggaatcgtt tgctctgtgt tggatacgga
      361 gacgaacgag ctagtagcga tgaagaagat agctaatgct tttgataatc atatggatgc
      421 taagcgtacg cttcgtgaga tcaagcttct tcgtcatctt gatcatgaaa acattatagc
      481 tataagagat gttgttccac caccactaag aaggcagttc agtgatgttt atatctctac
      541 tgaattaatg gatactgatc ttcatcaaat catcagatct aaccagagtt tatcagaaga
      601 acactgtcag tacttcttgt accagctact tcgaggactg aagtatatcc actcagctaa
      661 cattattcat agggatttaa agccgagcaa tcttctgttg aacgcgaatt gcgatttaaa
      721 gatttgtgat ttcggtcttg ctagacctac ttcagagaat gattttatga ctgagtatgt
      781 tgttacgaga tggtatagag cacctgagct tctgttgaac tcttcagatt acacagctgc
      841 tattgatgtt tggtctgttg gttgtatctt tatggagctt atgaatagaa agcctttgtt
      901 ccctggtaaa gaccatgttc atcaaatgcg cttattgaca gagttgcttg gcacaccgac
      961 agaatctgat ctcggtttta ctcacaatga ggatgcgaaa agatacatcc ggcaacttcc
     1021 caacttccca cgtcagccct tagctaaact tttctctcat gttaacccaa tggccattga
     1081 tcttgttgac agaatgttga cgtttgaccc caacagaaga atcactgttg aacaagctct
     1141 gaatcaccag taccttgcta aattgcacga cccgaatgat gagccaatct gtcaaaagcc
     1201 attctctttt gagttcgaac aacagcctct ggatgaggaa cagataaaag agatgatcta
     1261 ccaagaagcc atagcactca atccaacata cggttaggct ttgagaccac gaagtggctc
     1321 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1381 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1501 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
     1561 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     1621 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1681 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1741 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1801 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1861 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     1921 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     1981 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2041 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2101 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2161 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2221 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2281 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2341 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2401 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     2461 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     2521 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     2581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3361 tct
//
