LOCUS       sequence-207224-        3759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_MRAS-G22V
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1477..1611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1514..1533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1568..1587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1647..1666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1648..1666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1753..1770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1924..2512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2004..2023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2683..3474,3475..3543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3306..3325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3544..3648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3716..3734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgttgtatc tttcgttaag tgctggcgtc gagcagggct aacctcattt cgatttgacg
       61 attcgggtaa aaccgagcga tggacaggac ctggaaatgg gaaaggctta ctagaatcag
      121 ggtcgaacga gataagaaga tcaggattcg caaattagca cttggctttt ttacgtagaa
      181 acataggatg tccgctgagc cccttatata tacgtagtac gaatcaccgg cctggcgttg
      241 tataagcgag accagcgtgg acgaaaaggt aattgtttcc gaatggatcg cgttctctag
      301 aggatccggc aaagcttcgt gtattgtacc ggcccattgt caatcatgca aacttgatat
      361 tatattgaca agagaagaag gcagtttaaa ttaaaactct aaagtagaga gacattaatc
      421 tcagctgaca aggcaggtgg tcacagtaag ttcatttaaa tagttggcca acaatagcct
      481 ttccaagaaa gtatttttgt tccaggtcta tacaaaaata acacacaagc ggccgcaacc
      541 atggcgaccg tgccgaatca gaatgagaac ttaacgacat ataagttggt cgtggttggc
      601 gatgttggtg tcgggaagag cgcgctcacc atccaattct tccaaaagat gttcgttgaa
      661 gaatatgatc caactataga ggattcatat atccaacaca cagatatcga tggacagtgg
      721 tgtattttag atgtgctcga cacagccggc caggaggaat ttagtgcaat gcgtgaacaa
      781 tacatgagaa caggtgatgg tttccttatc gtgtacagcg taacggaccg cgcaagttac
      841 gatcacgtac cagagttcca cgatcttatt ctaagggtaa aagatgaaac acaattcccc
      901 gccctcctag tggccaacaa agttgatctt gtgcaccagc gagtggtgga agaagacgag
      961 gggaaggaaa tggcgcgaca attccagatt ccttacattg aaacgagcgc caaagaccct
     1021 ccgcaaaacg ttgataaagc tttccaagat attgttcgtt taataaggca acaaggtctt
     1081 caacaaaacg ggacgaccac cggaggtcgg gagagcaaag gatgttgtca ggttatgtag
     1141 ctccacctag aggccagaat tccagctgag cgccggtcgc taccattacc agttggtctg
     1201 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
     1261 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta
     1321 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca
     1381 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt
     1441 ccaaccgctg tttggtctgc tttctgacaa actcggaact tgtttattgc agcttataat
     1501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1561 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cgacaaagtc
     1621 aaagcggcca tcagatctgc cggtctccct atagtgagtc gtattaattt cgataagcca
     1681 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3661 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3721 gcgtatcacg aggcccttac gtattaatta aggcgcgcc
//
