LOCUS       sequence-207279-        4086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cGAL-N driver under the control of C. elegans hsp-16.41 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115."
COMMENT     Sequence Label: pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1795..1899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1900..1968,1969..2760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2118..2137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2931..3519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3420..3439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3673..3690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3807..3828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3843..3848,3849..3866,3867..3873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3881..3897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3886..3908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3905..3921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3905..3921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acgttgagct ggacggaaat agtggtaaag tgacatgatt atagtttgaa gatttctaat
       61 ttcacaatta gagcaaatgt tgttcggtat ttattttcaa cggtatttat actattttcc
      121 acctttttct agaacattcg agctgcttgt tgcaaaagga gggcgactca cattcggtac
      181 atggaaaagt agtgtacaca ataaagagac ccagatacat tttccgtctg cgtctctttg
      241 cacccaccgg gagtattttc aaacgaatgc atctaggacc ttctagaaca ttctgtaagg
      301 ctgcagaatg cgggtatata aggaaagcgg gctcagagga agccaacacg ctttgttcta
      361 gtgcatctaa aaaacttcga aaggcgcgcc tctagaggat ccccgggatt ggccaaagga
      421 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
      481 gctaggatgg gccctaaaaa gaagcgtaaa gctagcatga agctgttgtc ttcaatggaa
      541 caagcttgcg acatatgccg gttaaaaaaa ctcaggtgct cgaaggaaaa gcccaagtgt
      601 gccaagtgtc tgaagaacaa ctcggagtgc cgttattctc ccaaggctat gaggtcccca
      661 ctgaccaggg cgcatctgac agaggtagaa tcaaggttgg aaaaattgga agatttgttt
      721 ctgctcatgt ttcctcggga aaacctggac agcattttga acatggactc tttggacgaa
      781 gtgaaagtaa tgttgaaaca attatatatg caggactatg gtgacacgga tgctgctatg
      841 gatagcttga cccctatgga aaatggcatg tctgaagggc cgagaaaaca catgaagact
      901 gcgacatcat cgccaaaagt ggatgaagat ggaagccaaa gtcagttgag tgtatcgccg
      961 gaattcccgc tgcagtctgg tggcggaggg gctcctaggt gcctcgacct caagacccaa
     1021 gtccaaaccc cacaaggaat gaaggagatc tccaacatcc aagtcggaga cctcgtcctc
     1081 tccaacaccg gatacaacga ggtcctcaac gtcttcccaa agtccaagaa gaagtcctac
     1141 aagatcaccc tcgaggacgg aaaggagatc atctgctccg aggagcacct cttcccaacc
     1201 caaaccggag agatgaacat ctccggagga ctcaaggagg gaatgtgcct ctacgtcaag
     1261 gagtaaggta ccgaattcgc tagccggcca tacaagtaat ccggatgatc gacgccaacg
     1321 tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
     1381 tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt
     1441 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
     1501 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
     1561 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
     1621 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa
     1681 ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccca
     1741 aaaggccttt tttttttggc gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     3841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     3901 cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac
     3961 taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg
     4021 atggatacgc taacaacttg gaaatgaaat aagcttgcat gcgcggccgc actgactggg
     4081 ccggcc
//
