LOCUS       sequence-207288-        4666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115."
COMMENT     Sequence Label: pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2375..2479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2480..2548,2549..3340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2698..2717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3511..4099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4000..4019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4253..4270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4387..4408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4423..4428,4429..4446,4447..4453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4461..4477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4466..4488
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4485..4501
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4485..4501
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagtatcct ttgctttaaa tgtccataaa actaatttta taatcaataa aacaacgttt
       61 gtaaatcaac tgagtttaca agtagagaca ttgagggata ctttcactat gctaaagtga
      121 taatcgacca aataataact tcactttggt atttattcct gtcttataaa tgttatgtat
      181 gaattaaatt catatgcata tggctcactc tgacaatttt taataatctt ccagatcaat
      241 attgactacc gatgcgggtg gtcttttgct ttgaattctg ctgaacttta caccccgaac
      301 agcaatgtgt gcttcagcct aaaaaaaagt aagtgtgtta atcagtgccc ccgattcttc
      361 attttttgcc cctctctccc gtttcgtcgg caaaagaaga gaaaataaag ataagtctca
      421 agataggttg gtaatcgcta aagtggttgt gtggataaga gtagcaaaat ggcaggaaga
      481 gcactttgcg cgcacacact gtactcattg ttctggataa aattctctcg ttgtttgccg
      541 tcggatgtct gcctctctgc attgagccgg cttcttcact atctttagtt aacctaaaat
      601 gccgtttctt ttctcgtatc cccactatcc cgttgaggtt ctctgctctc ttcgctccct
      661 accgccagcg agcaactatc cgtgggggcg ccttgctcgg aagatggggg ggaagaaaga
      721 agatttttgc tatttgcact tgagaaagag acttttcctg cgtcgatggt tagagaacag
      781 tgtgcagaca cttttcagct acctagaatt acaattggat atccccgcct cccaatccac
      841 ccacccaggg aaaaagaagg gctcgccgaa aatcaaagtt atctccaggc tcgcgcatcc
      901 caccgagcgg ttgacttctc tccaccactt ttcattttaa ccctcgatcg tcagacacag
      961 aaggcgcgcc tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
     1021 gatactaaca taacatagaa cattttcagg aggacccttg gctaggatgg gccctaaaaa
     1081 gaagcgtaaa gctagcatga agctgttgtc ttcaatggaa caagcttgcg acatatgccg
     1141 gttaaaaaaa ctcaggtgct cgaaggaaaa gcccaagtgt gccaagtgtc tgaagaacaa
     1201 ctcggagtgc cgttattctc ccaaggctat gaggtcccca ctgaccaggg cgcatctgac
     1261 agaggtagaa tcaaggttgg aaaaattgga agatttgttt ctgctcatgt ttcctcggga
     1321 aaacctggac agcattttga acatggactc tttggacgaa gtgaaagtaa tgttgaaaca
     1381 attatatatg caggactatg gtgacacgga tgctgctatg gatagcttga cccctatgga
     1441 aaatggcatg tctgaagggc cgagaaaaca catgaagact gcgacatcat cgccaaaagt
     1501 ggatgaagat ggaagccaaa gtcagttgag tgtatcgccg gaattcccgc tgcagtctgg
     1561 tggcggaggg gctcctaggg ctctcgacct caagacccaa gtccaaaccc cacaaggaat
     1621 gaaggagatc tccaacatcc aagtcggaga cctcgtcctc tccaacaccg gatacaacga
     1681 ggtcctcaac gtcttcccaa agtccaagaa gaagtcctac aagatcaccc tcgaggacgg
     1741 aaaggagatc atctgctccg aggagcacct cttcccaacc caaaccggag agatgaacat
     1801 ctccggagga ctcaaggagg gaatgtgcct ctacgtcaag gagtaaggta ccgaattcgc
     1861 tagccggcca tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt
     1921 aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct
     1981 tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc
     2041 caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct
     2101 ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg
     2161 gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt
     2221 ttaaaagaaa acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag
     2281 aaattaggcg aacgatgaga attgtcctcg cttgggccca aaaggccttt tttttttggc
     2341 gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2401 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2461 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2521 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2581 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2641 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2701 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2761 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2821 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2881 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2941 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3001 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3061 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3121 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3181 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3241 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3301 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3361 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3421 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3481 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3541 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3601 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3661 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3721 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3781 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3841 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3901 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3961 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4021 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4081 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4141 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4201 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4261 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4321 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4381 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4441 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4501 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
     4561 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
     4621 gaaatgaaat aagcttgcat gcgcggccgc actgactggg ccggcc
//
