LOCUS       sequence-20730-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..239)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             298..375
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             403..423
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
       61 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
      121 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
      181 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
      241 atgggctgca ggaattcgat atcaagctta tcgataggtg gaccaggtgg tggaatgaag
      301 cgacgatgga aaaagaattt catagccgtc tcagcagcca accgctttaa gaaaatctca
      361 tcctccgggg cacttgatta tggtacccca actactgctt ctgaaaatct atattttcaa
      421 ggtgaactaa aaactgctgc tttggctcaa catgcgatta agggtgaagc tcaaaaactt
      481 aatgactctc aagctccaaa agctgatgcg caacaaaata acttcaacaa agatcaacaa
      541 agcgccttct atgaaatctt gaacatgcct aacttaaacg aagcgcaacg taacggcttc
      601 attcaaagtc ttaaagacga cccaagccaa agcactaacg ttttaggtga agctaaaaaa
      661 ttaaacgaat ctcaagcac
//
