LOCUS       sequence-207320-        8307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m 
            system for cellular recording in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT063a
FEATURES             Location/Qualifiers
     source          1..8307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..406
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    144..162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    252..270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     315..339
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             425..1111
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1160..4558,4559..4561,4562..5158,5159,5160..5162,
                     5163..5164,5165..5167,5168..5260)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1160..1183,1184..1186,1187..5260)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5273..5521
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5534..5554
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5563..5580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5603..5620)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5609..5833
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5904..5920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5904..5920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5917..5939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5928..5944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5952..5958,5959..5976,5977..5982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5997..6018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6135..6152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6306..6894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6386..6405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7065..7856,7857..7925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7688..7707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7926..8030)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8109..8128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggccctttc gtcttcaaga attcctcgag tttactccct atcagtgata gagaacgtat
       61 gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga
      121 tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact
      181 ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat
      241 atccagttta ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc
      301 gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca
      361 cttttgtctt ataccaactt tccgtaccac ttcctaccct cgtaaagtcg acagagccgc
      421 caccatgagc tcagagactg gcccagtggc tgtggacccc acattgagac ggcggatcga
      481 gccccatgag tttgaggtat tcttcgatcc gagagagctc cgcaaggaga cctgcctgct
      541 ttacgaaatt aattgggggg gccggcactc catttggcga catacatcac agaacactaa
      601 caagcacgtc gaagtcaact tcatcgagaa gttcacgaca gaaagatatt tctgtccgaa
      661 cacaaggtgc agcattacct ggtttctcag ctggagccca tgcggcgaat gtagtagggc
      721 catcactgaa ttcctgtcaa ggtatcccca cgtcactctg tttatttaca tcgcaaggct
      781 gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg gatttgatct cttcaggtgt
      841 gactatccaa attatgactg agcaggagtc aggatactgc tggagaaact ttgtgaatta
      901 tagcccgagt aatgaagccc actggcctag gtatccccat ctgtgggtac gactgtacgt
      961 tcttgaactg tactgcatca tactgggcct gcctccttgt ctcaacattc tgagaaggaa
     1021 gcagccacag ctgacattct ttaccatcgc tcttcagtct tgtcattacc agcgactgcc
     1081 cccacacatt ctctgggcca ccgggttgaa aagcggcagc gagactcccg ggacctcaga
     1141 gtccgccaca cccgaaagtg ataaaaagta ttctattggt ttagccatcg gcactaattc
     1201 cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat ttaaggtgtt
     1261 ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc tattcgatag
     1321 tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt atacacgtcg
     1381 caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca aagttgacga
     1441 ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga aacatgaacg
     1501 gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt acccaacgat
     1561 ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga ggttaatcta
     1621 cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg gtgatctaaa
     1681 tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct ataatcagtt
     1741 gtttgaagag aaccctataa atgcaagtgg cgtggatgcg aaggctattc ttagcgcccg
     1801 cctctctaaa tcccgacggc tagaaaacct gatcgcacaa ttacccggag agaagaaaaa
     1861 tgggttgttc ggtaacctta tagcgctctc actaggcctg acaccaaatt ttaagtcgaa
     1921 cttcgactta gctgaagatg ccaaattgca gcttagtaag gacacgtacg atgacgatct
     1981 cgacaatcta ctggcacaaa ttggagatca gtatgcggac ttatttttgg ctgccaaaaa
     2041 ccttagcgat gcaatcctcc tatctgacat actgagagtt aatactgaga ttaccaaggc
     2101 gccgttatcc gcttcaatga tcaaaaggta cgatgaacat caccaagact tgacacttct
     2161 caaggcccta gtccgtcagc aactgcctga gaaatataag gaaatattct ttgatcagtc
     2221 gaaaaacggg tacgcaggtt atattgacgg cggagcgagt caagaggaat tctacaagtt
     2281 tatcaaaccc atattagaga agatggatgg gacggaagag ttgcttgtaa aactcaatcg
     2341 cgaagatcta ctgcgaaagc agcggacttt cgacaacggt agcattccac atcaaatcca
     2401 cttaggcgaa ttgcatgcta tacttagaag gcaggaggat ttttatccgt tcctcaaaga
     2461 caatcgtgaa aagattgaga aaatcctaac ctttcgcata ccttactatg tgggacccct
     2521 ggcccgaggg aactctcggt tcgcatggat gacaagaaag tccgaagaaa cgattactcc
     2581 atggaatttt gaggaagttg tcgataaagg tgcgtcagct caatcgttca tcgagaggat
     2641 gaccaacttt gacaagaatt taccgaacga aaaagtattg cctaagcaca gtttacttta
     2701 cgagtatttc acagtgtaca atgaactcac gaaagttaag tatgtcactg agggcatgcg
     2761 taaacccgcc tttctaagcg gagaacagaa gaaagcaata gtagatctgt tattcaagac
     2821 caaccgcaaa gtgacagtta agcaattgaa agaggactac tttaagaaaa ttgaatgctt
     2881 cgattctgtc gagatctccg gggtagaaga tcgatttaat gcgtcacttg gtacgtatca
     2941 tgacctccta aagataatta aagataagga cttcctggat aacgaagaga atgaagatat
     3001 cttagaagat atagtgttga ctcttaccct ctttgaagat cgggaaatga ttgaggaaag
     3061 actaaaaaca tacgctcacc tgttcgacga taaggttatg aaacagttaa agaggcgtcg
     3121 ctatacgggc tggggacgat tgtcgcggaa acttatcaac gggataagag acaagcaaag
     3181 tggtaaaact attctcgatt ttctaaagag cgacggcttc gccaatagga actttatgca
     3241 gctgatccat gatgactctt taaccttcaa agaggatata caaaaggcac aggtttccgg
     3301 acaaggggac tcattgcacg aacatattgc gaatcttgct ggttcgccag ccatcaaaaa
     3361 gggcatactc cagacagtca aagtagtgga tgagctagtt aaggtcatgg gacgtcacaa
     3421 accggaaaac attgtaatcg agatggcacg cgaaaatcaa acgactcaga aggggcaaaa
     3481 aaacagtcga gagcggatga agagaataga agagggtatt aaagaactgg gcagccagat
     3541 cttaaaggag catcctgtgg aaaataccca attgcagaac gagaaacttt acctctatta
     3601 cctacaaaat ggaagggaca tgtatgttga tcaggaactg gacataaacc gtttatctga
     3661 ttacgacgtc gatcacattg taccccaatc ctttttgaag gacgattcaa tcgacaataa
     3721 agtgcttaca cgctcggata agaaccgagg gaaaagtgac aatgttccaa gcgaggaagt
     3781 cgtaaagaaa atgaagaact attggcggca gctcctaaat gcgaaactga taacgcaaag
     3841 aaagttcgat aacttaacta aagctgagag gggtggcttg tctgaacttg acaaggccgg
     3901 atttattaaa cgtcagctcg tggaaacccg ccaaatcaca aagcatgttg cacagatact
     3961 agattcccga atgaatacga aatacgacga gaacgataag ctgattcggg aagtcaaagt
     4021 aatcacttta aagtcaaaat tggtgtcgga cttcagaaag gattttcaat tctataaagt
     4081 tagggagata aataactacc accatgcgca cgacgcttat cttaatgccg tcgtagggac
     4141 cgcactcatt aagaaatacc cgaagctaga aagtgagttt gtgtatggtg attacaaagt
     4201 ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag ataggcaagg ctacagccaa
     4261 atacttcttt tattctaaca ttatgaattt ctttaagacg gaaatcactc tggcaaacgg
     4321 agagatacgc aaacgacctt taattgaaac caatggggag acaggtgaaa tcgtatggga
     4381 taagggccgg gacttcgcga cggtgagaaa agttttgtcc atgccccaag tcaacatagt
     4441 aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa tcgattcttc caaaaaggaa
     4501 tagtgataag ctcatcgctc gtaaaaagga ctgggacccg aaaaagtacg gtggcttcga
     4561 tagccctaca gttgcctatt ctgtcctagt agtggcaaaa gttgagaagg gaaaatccaa
     4621 gaaactgaag tcagtcaaag aattattggg gataacgatt atggagcgct cgtcttttga
     4681 aaagaacccc atcgacttcc ttgaggcgaa aggttacaag gaagtaaaaa aggatctcat
     4741 aattaaacta ccaaagtata gtctgtttga gttagaaaat ggccgaaaac ggatgttggc
     4801 tagcgccgga gagcttcaaa aggggaacga actcgcacta ccgtctaaat acgtgaattt
     4861 cctgtattta gcgtcccatt acgagaagtt gaaaggttca cctgaagata acgaacagaa
     4921 gcaacttttt gttgagcagc acaaacatta tctcgacgaa atcatagagc aaatttcgga
     4981 attcagtaag agagtcatcc tagctgatgc caatctggac aaagtattaa gcgcatacaa
     5041 caagcacagg gataaaccca tacgtgagca ggcggaaaat attatccatt tgtttactct
     5101 taccaacctc ggcgctccag ccgcattcaa gtattttgac acaacgatag atcgcaaacg
     5161 atacacttct accaaggagg tgctagacgc gacactgatt caccaatcca tcacgggatt
     5221 atatgaaact cggatagatt tgtcacagct tgggggtgac tctggtggtt ctactaatct
     5281 gtcagatatt attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat
     5341 gctcccagag gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca
     5401 caccgcctac gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga
     5461 atacaagcct tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct
     5521 ctctggtggt tctcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat
     5581 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5641 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5701 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5761 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5821 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct
     5881 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga
     6001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8041 cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtcg
     8101 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8161 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     8221 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     8281 cgggccagat atacgcgttg acattga
//
