LOCUS       sequence-207342-        9008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Calcium channel beta4 auxillary subunit in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Brodbeck J, Davies A, Courtney JM, Meir A, Balaguero N, Canti C, 
            Moss FJ, Page KM, Pratt WS, Hunt SP, Barclay J, Rees M, Dolphin AC
  TITLE     The ducky mutation in Cacna2d2 results in altered Purkinje cell 
            morphology and is associated with the expression of a truncated 
            alpha 2 delta-2 protein with abnormal function.
  JOURNAL   J Biol Chem. 2002 Mar 8;277(10):7684-93. doi: 
            10.1074/jbc.M109404200. Epub 2001 Dec 26.
  PUBMED    11756448
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109404200"; journalName: "J Biol Chem"; date: 
            "2002-03-8- 8"; volume: "277"; issue: "10"; pages: "7684-93"
COMMENT     Sequence Label: cacnb4 (rat) in pMT2 vector
FEATURES             Location/Qualifiers
     source          1..9008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..648
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    835..969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(872..891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     926..945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1642..1659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1813..2401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1893..1912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2572..3363,3364..3432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3195..3214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3433..3537)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3605..3623
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3661..3683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3783..3802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3996..4018
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4010..4027
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4011..4027
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4037..4366)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4051..4186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4351..4371
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          4835..5064
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5047..5066
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     5127..5143
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5128..5144
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggacagctca gggctgcgat ttcgcgccaa acttgacggc aatcctagcg tgaaggctgg
       61 taggatttta tccccgctgc catcatggtt cgaccattga actgcatcgt cgccgtgtcc
      121 caaaatatgg ggattggcaa gaacggagac ctaccctggc ctccgctcag gaacgagttc
      181 aagtacttcc aaagaatgac cacaacctct tcagtggaag gtaaacagaa tctggtgatt
      241 atgggtagga aaacctggtt ctccattcct gagaagaatc gacctttaaa ggacagaatt
      301 aatatagttc tcagtagaga actcaaagaa ccaccacgag gagctcattt tcttgccaaa
      361 agtttggatg atgccttaag acttattgaa caaccggaat tggcaagtaa agtagacatg
      421 gtttggatag tcggaggcag ttctgtttac caggaagcca tgaatcaacc aggccacctc
      481 agactctttg tgacaaggat catgcaggaa tttgaaagtg acacgttttt cccagaaatt
      541 gatttgggga aatataaact tctcccagaa tacccaggcg tcctctctga ggtccaggag
      601 gaaaaaggca tcaagtataa gtttgaagtc tacgagaaga aagactaaca ggaagatgct
      661 ttcaagttct ctgctcccct cctaaagcta tgcattttta taagaccatg ggacttttgc
      721 tggctttaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      781 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      961 gtctggatcc ccggccaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac
     1021 gcgagtaagc ccttgagtca aagacgtagt cgttgcaagt ccgcaccagg tactgatatc
     1081 ccaccaaaaa gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc
     1141 cgggggcgag gtcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg
     1201 tgatgccggc ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc
     1261 gcagcggcaa aaagtgctcc atggtcggga cgctctggcc ggtgaggcgt gcgcagtcgt
     1321 tgacgctcta gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg
     1381 gataaattcg caagggtatc atggcggacg accggggttc gaaccccgga tccggccgtc
     1441 cgccgtgatc catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac
     1501 gggggagcgc tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg
     1561 cgagctcgaa ttaattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1621 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1681 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1741 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1801 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1861 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1921 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1981 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2041 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2101 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2161 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2221 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2281 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2341 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2401 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2461 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2521 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2581 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2641 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2701 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2761 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2821 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2881 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2941 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3001 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3061 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3121 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3181 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3241 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3301 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3361 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3421 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3481 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3541 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3601 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3661 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3721 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3781 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3841 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     3901 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3961 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     4021 ggccagtgcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4081 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4141 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4201 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4261 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4321 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacaggatcc
     4381 ggtcgcgcga atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc
     4441 tgagacgaag gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc
     4501 gttgtccact agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc
     4561 atcaaggaag gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg
     4621 gctataaaag ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag
     4681 ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg
     4741 atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc
     4801 atcgaccgga tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc
     4861 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     4921 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     4981 ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca
     5041 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga
     5101 attcctcgag ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
     5161 attggcctct ctgggcgaat tccgggtgct ctccccaccc cgcgaggggg gaggggagcg
     5221 cttcggcccc tttaaggtgc ggcgctcggc gaagtgcggg caggcggccc gcgagccggc
     5281 gcggggccag gagccgggta gggaagtcct gcctccccct ccgcccgcac cggcccgcgc
     5341 cctccgccca ccgcgccgcg agggccatgc ggcgcctctg aaccatgtcg tcctcctacg
     5401 ccaagaacgg ggcggcggac gggccgcact cccccagctc gcaggtggcc cgaggcacca
     5461 cgacccggag gagcaggttg aaaagatccg atggcagcac cacttccacc agcttcatcc
     5521 tcagacaggg gtcagcagat tcctatacaa gcaggccatc ggactccgat gtctctttgg
     5581 aagaggaccg ggaagcgatt cggcaagaga gagaacagca agcagctatc cagcttgaga
     5641 gagcgaagtc caaacctgta gcttttgcgg tgaaaacgaa tgtgagctac tgtggtgccc
     5701 tggatgagga tgtgcccgtt cccagcacag ccatctcctt tgacgccaag gactttcttc
     5761 acattaaaga gaaatataac aatgattggt ggataggaag gctggtgaag gagggctgtg
     5821 agatcggctt catcccaagt ccactgcgtt tggagaacat acggattcaa caggaacaga
     5881 aaagaggccg ttttcatggc gggaaatcga gtggaaactc ttcctccagt ctgggagaaa
     5941 tggtgtcagg aacatttcga gcaactccca caacaacagc aaaacagaag cagaaagtga
     6001 cggagcacat tcctccgtat gatgttgtgc catcaatgcg tcctgtggtg ttagtagggc
     6061 catcactgaa aggttatgag gtaacagaca tgatgcagaa agccctcttt gatttcctga
     6121 agcacaggtt tgatgggagg atatcaataa ccagagtgac agctgacatt tctcttgcta
     6181 agaggtctgt cctcaacaat cctagcaaga gagcaataat tgaacgttca aacaccagat
     6241 ccagcctagc ggaagtacag agtgaaattg aaagaatttt cgagttggca agatctttgc
     6301 aattggttgt tctcgatgca gacaccatca atcacccagc acagctgata aagacatcgt
     6361 tagcgcccat catcgtccac gtgaaggtct cgtccccaaa ggttttacag cggctgatta
     6421 agtccagagg aaagtcccag agtaaacact tgaatgttca actggtggca gctgataaac
     6481 tggcccagtg tccccctgaa atgtttgatg ttatattaga tgagaatcaa cttgaggatg
     6541 cctgtgaaca tctgggagag tacctggagg catattggcg tgccacccac acaagcagta
     6601 gcacccctat gaccccattg ctggggagga acgtgggctc cacagctctc tcaccgtatc
     6661 ccacagcaat ctctggacta cagagtcagc ggatgaggca tagcaaccac tctacagaga
     6721 attctccaat tgaaagacga agcctaatga cctctgatga aaattaccac aatgagaggg
     6781 cccgcaagag taggaaccgc ttgtcctcca gctcccagca cagccgagat cactaccctc
     6841 tggtggaaga agattatcct gactcatacc aggacactta caaaccccat aggaaccgag
     6901 gctcacctgg gggatgcagc catgactccc gacataggct ttgaggctgc ccaaacaaac
     6961 aaacaaacaa acaaacatca tctgttgaca atttgccata gtactgctaa gataaaccag
     7021 tcatcttaat ttggcaagca tggcacagta tctgctgtgc gggtggtctg ctgtcgtttg
     7081 atgtaagggg caagaattaa atagaagtac attatgccct tgagtctagg agggtattag
     7141 atacccggtc atataggtat ttttaagtgc tacatttaca tgataacagt accttttgtt
     7201 ttcttcatag attttagaca catcaatttg taatttaggg tactgatcaa ggcacatata
     7261 aaataatttc ccgtgtagga aattccaaat gaccagttcc agttggaacc aatttataaa
     7321 ccaattcctg ttggaatttg ggtgaattga ctggaaagtc tacctgagca tgttgaaatc
     7381 aattgaaaaa atcagagaag aaaaaaatac ccaataagga aatcaaaata ctaatagaaa
     7441 ccaaacccag ctatggtatt tgtttgctgg aagctaaatt tacacttcaa gttggaatgt
     7501 agaaacattt taataatgtt gctggaatgg cttttcaaac ttaccaaata tattgatagt
     7561 gccaaaaaaa aatctcagaa atcttaatgc agaaattata taatcgcctc agattttaat
     7621 atttttattt ttcctgatat agctgttgta ttcaaagtgt tttgccttct gttcagtcag
     7681 tactgttagc cagtttgaag ttgacaaaat ccaggggata tttgagaagt ggtaaagaga
     7741 agccctagca tggacagttc cagagagtgg agaatgaggt gttgttcctt ctgcagtcag
     7801 tctgcacaca gctgcacaca tctggagacc tggtgtcagg tagcagagat gtggcggggc
     7861 ctctctggct acaacggtaa ttggcttcag atgggaagtc gtccatcgta cagcacaccg
     7921 acacgggctt aggagctgtg ccagcatggc tgagtgtctt tccctcactt cagccagtct
     7981 ccatctttga atataatgaa tgttttggcc ctgcatcaca cttcttttat gtttcatggg
     8041 cataatcaaa ttaggtactg aaagttatgt tttcacaaaa taattaggaa aacttaaaac
     8101 actgaattaa aatcttgcag agaaagaaaa aaaaaccctc atagaatcag aatatattta
     8161 ttcactaaaa gcaagacagt ttcaatacac ggcattagag agagttgaga gactgctcgg
     8221 ttccattgat agtgaacagc ctttagactt ctatgagtgt atttatggaa ttgatgacct
     8281 atgtcctatg ttattaaaga cagctttaat ccaagaaatt agtgaaattg accctttgaa
     8341 acaaatgtct ttctgaaagg gcttgtttct gaaatgacgc aatacatgtg ttttctaaag
     8401 acccaattgg agctagacgt aaagtctctt tctactaatg ctttgaagtc aatttccgaa
     8461 acaagtataa ttaatagtat gttaatttct ccaaaaaaaa gacatgggtc ctcaaaactg
     8521 tgtaccagtt cattaattct cctgatcaag agtctaagtt tcatattctt aatcagttat
     8581 cctatctcgg atcccatcaa cccattgtct tgactgtatg tgcaacccat tatgaacctg
     8641 cttatctgat aatgaggctg cctgaaattt tgtggtaatc tcaaacattt gatacattta
     8701 aacacgggct cagtcaaatt gacaagctaa catctgacac tggcttgtga agcttttttg
     8761 tcatccagcg aggcttacag aattttctgt taagacattt aacagagctg tattcattgg
     8821 gctctttaat aagcatataa tcttccatta ccaaatactt tgttagaaga cagaagagca
     8881 tttttccagc gaacaaacta tatttattac atgtatagca catatttgca tgagaaagaa
     8941 aaacattagc caaacatttt cctgtatgct ttactggttt cttgtttgtg cgcattaaag
     9001 catttaat
//
