LOCUS       sequence-207367-        3290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG659
FEATURES             Location/Qualifiers
     source          1..3290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..23
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     257..274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             358..1287
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="TSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1297..1386
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(1431..1449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1517..1621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1843..2634
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2805..3290,1..103)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
       61 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      301 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ttcggctact
      361 tcgaaagttt atgatccaga acaaaggaaa cggatgataa ctggtccgca gtggtgggcc
      421 agatgtaaac aaatgaatgt tcttgattca tttattaatt attatgattc agaaaaacat
      481 gcagaaaatg ctgttatttt tttacatggt aacgcggcct cttcttattt atggcgacat
      541 gttgtgccac atattgagcc agtagcgcgg tgtattatac cagaccttat tggtatgggc
      601 aaatcaggca aatctggtaa tggttcttat aggttacttg atcattacaa atatcttact
      661 gcatggtttg aacttcttaa tttaccaaag aagatcattt ttgtcggcca tgattggggt
      721 gcttgtttgg catttcatta tagctatgag catcaagata agatcaaagc aatagttcac
      781 gctgaaagtg tagtagatgt gattgaatca tgggatgaat ggcctgatat tgaagaagat
      841 attgcgttga tcaaatctga agaaggagaa aaaatggttt tggagaataa cttcttcgtg
      901 gaaaccatgt tgccatcaaa aatcatgaga aagttagaac cagaagaatt tgcagcatat
      961 cttgaaccat tcaaagagaa aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa
     1021 atcccgttag taaaaggtgg taaacctgac gttgtacaaa ttgttaggaa ttataatgct
     1081 tatctacgtg caagtgatga tttaccaaaa atgtttattg aatcggaccc aggattcttt
     1141 tccaatgcta ttgttgaagg tgccaagaag tttcctaata ctgaatttgt caaagtaaaa
     1201 ggccttcatt tttcgcaaga agatgcacct gatgaaatgg gaaaatatat caaatcgttc
     1261 gttgagcgag ttctcaaaaa tgaacaatct gctgcctacc catacgatgt tcctgactat
     1321 gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat
     1381 tacgctgcct agctttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     1441 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1501 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1621 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1681 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     1741 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1801 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1861 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1921 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     1981 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2041 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2101 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2161 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2221 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2281 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2341 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2401 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2461 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2521 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2581 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     2641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
//
