LOCUS       sequence-20753-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..155)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(163..179)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(163..179)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(194..635)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(200..223)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    518..635
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag
       61 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      121 tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg gggatccact agttctagaa
      181 tccggggttt tttctccttg acgttaaagt atagaggtat attaacaatt ttttgttgat
      241 acttttatta catttgaata agaagtaata caaaccgaaa atgttgaaag tattagttaa
      301 agtggttatg cagtttttgc atttatatat ctgttaatag atcaaaaatc atcgcttcgc
      361 tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc tatggttgtt
      421 aatttgattc gttcatttga aggtttgtgg ggccaggtta ctgccaattt ttcctcttca
      481 taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac
      541 atctgcgttt caggaacgcg accggtgaag acgaagacgc acggaggaga gtcttccttc
      601 ggaaggctgt cacccgctcg gcggcttcta atccgta
//
