LOCUS       sequence-207530-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KALRN (KALRNA-c001)
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca tcatcatcat cattcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccat gcgcaagaaa gagttcatca tggctgaact tctgcaaacc
      121 gagaaagcgt atgttcgcga tttacacgaa tgtctggaaa cgtatctgtg ggaaatgacc
      181 tcaggagtgg aagagattcc tccagggatt ctgaacaaag agcacattat ctttggcaat
      241 attcaggaaa tctacgactt tcacaacaac atctttctca aagaactcga gaaatacgaa
      301 caactgcccg aagatgtggg tcattgcttt gtgacatggg cagacaaatt ccagatgtac
      361 gtcacctatt gcaagaacaa accggattcg aatcaactga ttctggaaca tgccggtacg
      421 ttctttgacg aaattcagca gcgtcatggc ttagccaatt ccatttctag ctatctgatc
      481 aaaccggtac agcgtgtcac caaatatcag ttgcttctga aagaattgct gacttgttgc
      541 gaggaaggta aaggcgaatt gaaggatggc ttagaggtga tgctgagtgt tccgaagaaa
      601 gcgaatgatg cgatgcatgt atgacagtaa aggtggatac ggatccgaa
//
