LOCUS       sequence-207541-        2067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2067)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ANO10 (TMEM16KA-c001)
FEATURES             Location/Qualifiers
     source          1..2067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1984..2004
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2005..2034
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2035..2058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgaaagtga cgttatcagc tttggatact tctgagagtt ctttcacacc tttggtggtc
       61 atagaacttg ctcaggatgt caaagaagaa accaaagaat ggctgaaaaa cagaattata
      121 gctaaaaaaa aagatggagg tgcccagttg ttgtttagac cattgttaaa taaatatgaa
      181 caagaaacac tagaaaatca gaacttatat cttgttggtg cctccaagat tagaatgtta
      241 ctaggggcag aagcagtggg attggtaaaa gagtgcaatg ataacaccat gagagccttc
      301 acatacagaa ccagacagaa cttcaaaggt tttgatgata acaatgatga tttcctgaca
      361 atggcagaat gtcaattcat tatcaaacat gaacttgaaa atcttagagc taaagatgaa
      421 aaaatgatcc ctggttaccc tcaggcaaag ttgtatccag gaaaatcatt gttgagaaga
      481 ttgctcacgt ctggcatcgt gattcaggtg tttccactgc atgacagtga agccctgaag
      541 aagcttgagg acacctggta cactcggttt gctttgaagt atcagcccat agacagtatt
      601 cgtggctact ttggggaaac aattgctctg tactttggat ttttggagta tttcactttt
      661 gcattaatcc ccatggctgt cattgggtta ccttactact tgtttgtgtg ggaagactat
      721 gacaagtacg tgatctttgc ctcgttcaac ctcatctggt ccacggtgat tctggaactg
      781 tggaagcgtg gctgtgccaa catgacctac aggtggggga cactgctcat gaagagaaag
      841 tttgaggagc cccggccagg atttcatggt gtcttgggta tcaattccat cactgggaag
      901 gaggagcctc tgtaccccag ctacaagaga cagttgcgca tttacctggt ctccctgcca
      961 ttcgtgtgcc tctgcctcta tttctcactg tatgtcatga tgatttactt cgacatggag
     1021 gtttgggcct tgggtctaca tgagaacagc gggtctgagt ggaccagtgt cctgttgtat
     1081 gtgcccagca tcatctatgc cattgtgatt gagatcatga atcgtctcta tcgatatgct
     1141 gccgagtttt taacttcatg ggagaatcac agattggaat ctgcctatca gaaccatcta
     1201 attctgaaag ttttagtgtt caacttcctc aattgctttg cctcactctt ctatattgcc
     1261 tttgtcttga aagatatgaa gcttttgcgc cagagcttgg ccactctcct aattacctcc
     1321 cagatcctca accaaattat ggaatctttt cttccttatt ggctccaaag gaagcatggt
     1381 gtgcgggtga agaggaaggt gcaggcttta aaggcagaca ttgatgctac attatatgaa
     1441 caagtcatcc tggaaaaaga aatgggaact tatttgggca cctttgatga ttacttggag
     1501 ttattcctgc agtttggtta tgtgagcctt ttctcctgtg tttacccatt agcagctgcc
     1561 tttgctgtgt taaataactt cactgaagta aattcagatg ccttaaaaat gtgcagggtc
     1621 ttcaaacgtc cattctcaga accttcagcc aatattggtg tgtggcagtt ggcttttgaa
     1681 acgatgagtg ttatatctgt ggtcactaac tgtgcgctga ttggaatgtc accacaagtg
     1741 aatgcagtct ttccagaatc aaaagcagac ctcattttga ttgtagtagc agtggagcac
     1801 gcactcctgg ctttaaagtt tatacttgca tttgccatac ctgataagcc acggcatatc
     1861 cagatgaaac tagccagact ggaatttgag tctttggagg cactcaagca gcagcaaatg
     1921 aagctcgtga ccgagaacct gaaggaggaa ccaatggaaa gcgggaagga gaaggcaacc
     1981 gcagagaacc tctacttcca atcgcaccat catcaccatc accatcacca ccatgattac
     2041 aaggatgacg acgataagtg aggatcc
//
