LOCUS       sequence-207629-       11706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal
            super-folder GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: K785R Smarca4-sfGFP
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(4870..4872,4873..5586)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4912..4933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5520..5541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5617..6205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5670..5690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6088..6099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6292..6525
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6597..6718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6634..6653)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6688..6707
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6758..6893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6913..6932)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6914..6932)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6942..6959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6942..6958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6951..6973)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7100..7555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7187..7206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7397..7418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7581..7685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7686..7754,7755..8546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7904..7923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8717..9305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9206..9225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9459..9476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9593..9614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9629..9634,9635..9652,9653..9659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9667..9683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9672..9694
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9691..9707
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9691..9707
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9726..9746
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9728..9746
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9774..10000
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10001..10181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10228..10353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10846..11079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11264..11308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11575..11692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgtctactc cagacccacc cttgggtggg actcctcggc ctggtccttc cccaggccct
       61 ggtccttcac ctggtgcaat gctgggtcct agccctggcc cctcaccagg ttctgcccac
      121 agcatgatgg ggccaagccc aggacctcct tcagcaggac atcccatgcc cacccagggg
      181 cctggagggt acccccagga caacatgcat cagatgcaca agcctatgga gtccatgcac
      241 gagaagggca tgcctgatga cccacgatac aaccagatga aagggatggg catgcggtca
      301 ggggcccaca caggcatggc acctccacct agtcccatgg accagcattc tcaaggttac
      361 ccctcacccc tcggcggctc tgaacatgcc tccagtcctg tcccagccag tggcccatct
      421 tcaggccccc agatgtcctc tgggccagga ggggccccac tagatggttc tgatccccag
      481 gccttgggac agcaaaacag aggcccaacc ccatttaacc agaaccagct gcatcaactc
      541 agagctcaga taatggccta caagatgttg gccaggggcc agccattgcc cgaccacctg
      601 cagatggccg tgcaaggcaa gcggccgatg cctggaatgc agcaacagat gccaacacta
      661 cctccaccct cagtgtccgc cacaggaccc ggacctggac ccggccctgg ccctggccct
      721 ggcccaggac cagcccctcc aaattacagt agaccccatg gtatgggagg gcccaacatg
      781 cctcccccag gaccctcagg tgtgcccccc gggatgcctg gtcagccgcc tggagggcct
      841 cccaagccat ggcctgaagg acccatggcc aatgctgctg cccccacaag caccccacag
      901 aagctgattc ctccgcaacc aacaggccgt ccttcacctg cacctcctgc tgtcccgcct
      961 gctgcctcac ctgtaatgcc accacaaaca cagtccccag ggcagccagc ccagcctgct
     1021 ccattggtgc cactgcacca gaagcagagc cgaatcaccc ccatccagaa gccccgaggc
     1081 cttgaccctg tggagatcct acaagagcgg gagtacaggc ttcaggctcg aatcgcacac
     1141 agaattcagg aacttgaaaa cctccctggg tccctggctg gggaccttcg aaccaaagca
     1201 accatcgaac tcaaggccct taggttgctg aacttccaga ggcagctgcg ccaggaggtg
     1261 gtggtgtgca tgcgaagaga cacagccctg gagacagccc tcaatgccaa ggcctacaag
     1321 cgcagcaaac gtcagtcact acgggaggcc cgcatcactg agaagttgga gaagcagcag
     1381 aagattgaac aggagcgcaa gcgccgccag aagcaccagg agtacctcaa cagcattctg
     1441 cagcatgcca aggacttcag ggagtatcac agatcagtca caggcaaact ccagaaactc
     1501 accaaggctg tggccaccta ccatgccaac actgagcggg agcagaagaa agaaaatgag
     1561 cgcattgaga aggagcgaat gcggaggctt atggctgaag atgaggaggg ctaccgcaaa
     1621 ctcattgacc agaagaagga caagcgcctg gcctaccttc tgcagcagac agatgagtat
     1681 gtggccaacc tcacagagct ggtgcggcag cacaaagctg cccaggttgc caaggagaag
     1741 aagaagaaaa agaaaaagaa gaaggcagaa aatgctgaag gacagacacc tgctattgga
     1801 ccagatggtg agcctctgga tgagaccagc cagatgagtg acctccctgt gaaggtgatc
     1861 cacgtggaga gtggcaagat cctcactggc acagatgccc caaaagccgg gcagctggaa
     1921 gcctggcttg aaatgaaccc agggtatgaa gtagccccca ggtcagacag tgaagaaagt
     1981 ggctctgaag aggaggagga ggaggaggaa gaggagcagc ctcagcccgc acagccccct
     2041 acactgcctg tggaagaaaa gaagaagatt ccagacccag acagcgatga tgtctctgag
     2101 gtggacgccc gacacattat tgagaacgcc aagcaagatg tggacgatga gtacggtgtg
     2161 tcccaggccc ttgctcgtgg cctgcagtct tactatgctg tggcccatgc agtcacagag
     2221 agagtagata agcagtccgc cctcatggtc aacggtgtcc tcaaacagta ccagatcaag
     2281 ggtttggagt ggctggtgtc cctgtacaac aacaacctga atggcatcct ggctgatgag
     2341 atggggctgg ggaggaccat ccagaccatc gcgctcatca catacctcat ggagcacaag
     2401 cgcatcaacg ggcctttcct catcatcgtg cctctctcga cactgtcaaa ctgggcgtat
     2461 gaatttgaca agtgggcccc ctctgtggtg aaggtttctt acaagggctc tccagctgca
     2521 aggcgagctt ttgtcccaca gcttcgcagt gggaagttca acgtcttact gaccacctat
     2581 gaatatatca tcaaagacaa gcatatccta gccaagatcc gctggaagta catgattgtg
     2641 gatgaaggcc accgcatgaa aaaccaccac tgcaagttga cgcaggtcct taacacacac
     2701 tacgtggccc ctcggcgcct gcttcttaca ggcacaccac tgcagaacaa gctaccggag
     2761 ctctgggccc tgcttaactt cctgctcccc actatcttca agagctgcag caccttcgaa
     2821 cagtggttca atgcaccctt tgccatgact ggagaaaagg tggacctgaa tgaagaggag
     2881 actatcctca ttattcgtcg cctacacaaa gttctgcggc ccttcctgct gcggcggctc
     2941 aagaaggaag ttgaagccca gctccctgag aaggtagagt atgtcatcaa atgcgacatg
     3001 tcagccctgc agcgtgtgct gtaccgtcac atgcaggcca aaggtgtgct gctgactgac
     3061 ggctccgaga aggacaagaa gggcaaaggt ggcaccaaga cactgatgaa cactattatg
     3121 caactgcgta agatctgcaa ccacccctac atgttccagc acatcgagga gtccttttct
     3181 gagcacttgg ggttcaccgg cggcatcgtg caaggattgg acctttaccg tgcctcaggg
     3241 aaatttgaac ttcttgatag aattctaccc aaactccgtg caacgaacca taaagtgctc
     3301 ctcttttgcc aaatgacctc cctcatgacc atcatggaag actactttgc ataccgtggc
     3361 ttcaaatacc tcaggcttga tggaaccaca aaagcagaag accggggcat gctgttgaaa
     3421 acctttaatg aacctggctc tgagtatttc attttcctgc tcagtacccg tgctgggggg
     3481 ctgggcctga atctgcagtc agctgacact gtgatcatct ttgacagtga ctggaatccc
     3541 caccaggacc tgcaagcaca ggatcgagcc catcgcattg gacagcagaa tgaggtgcgt
     3601 gttcttcgcc tgtgcacggt caacagtgtg gaagagaaga tactggctgc tgccaaatac
     3661 aaactcaatg tggatcagaa ggtgatccag gcaggcatgt tcgaccagaa gtcgtccagc
     3721 catgagaggc gtgccttcct gcaggccatc ctggagcacg aggagcagga tgaggaggaa
     3781 gatgaggtgc ctgatgatga gaccgtcaac cagatgattg cccggcacga agaagagttt
     3841 gacctcttca tgcgcatgga cttggaccgc cggcgtgaag aagcccgcaa ccccaagcgg
     3901 aagccacgcc tgatggaaga ggatgagctc ccatcctgga tcatcaagga tgatgccgag
     3961 gtggagcggc tgacatgtga agaggaagag gagaagatgt tcggccgtgg ttctcgccac
     4021 cgcaaggagg tagactacag cgactcactg acagagaagc agtggctcaa ggctatcgag
     4081 gagggcacgc tggaggagat cgaagaggag gtccggcaga agaaatcttc acgtaagcgt
     4141 aagcgagaca gcgaggccgg ctcctccacc ccgaccacca gcacccgcag ccgtgacaag
     4201 gatgaggaga gcaagaagca gaagaaacgt gggcggccac ctgctgagaa gctgtcccca
     4261 aacccaccta acctcaccaa gaagatgaag aagatcgtgg atgctgtgat caagtacaaa
     4321 gacagcagca gtggacgtca gctcagcgag gtgttcatcc agctcccctc tcgcaaggag
     4381 cttcctgagt actatgagct catccgaaag cctgtggact tcaagaagat caaggaacgc
     4441 atccgaaacc acaagtaccg cagcctcaat gacctggaga aggatgtgat gctgctgtgc
     4501 cagaacgctc agacgttcaa cctcgagggt tccctgatct atgaggactc catcgtcctg
     4561 cagtctgtct tcaccagcgt acggcagaag attgagaagg aggacgacag tgaaggcgag
     4621 gaaagcgagg aggaggagga gggcgaggag gaaggctccg agtctgagtc ccgctccgtc
     4681 aaggtgaaga tcaagctggg ccgcaaggag aaggcccagg accgactcaa ggggggccgc
     4741 cggcggccaa gccggggatc ccgggccaag ccggttgtga gtgacgatga cagtgaggag
     4801 gagcaggagg aggaccgctc aggaagtggc agtgaggaag acggtggaac cggtagtgga
     4861 ggatccggtg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4921 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     4981 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5041 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5101 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5161 atctccttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     5221 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5281 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcacggc cgacaagcag
     5341 aagaacggca tcaaggcgaa cttcaagatc cgccacaacg tcgaggacgg cagcgtgcag
     5401 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5461 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     5521 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5581 aagtaagtcg acgatatcgt acaagtaaga ttcgacaatc aacctctgga ttacaaaatt
     5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6001 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6181 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     6241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6301 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6541 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6601 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6661 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6721 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6841 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6901 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6961 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7021 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7081 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7141 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7201 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7261 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7321 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7381 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7441 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7501 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7561 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7621 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7681 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7741 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7801 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7861 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7921 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7981 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8041 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8101 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8161 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8221 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8281 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8341 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8401 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8461 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8521 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8581 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8641 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9361 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9421 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9481 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9541 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9601 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9661 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9721 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     9781 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     9841 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     9901 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     9961 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
    10021 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10081 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10141 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10201 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10261 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10321 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10381 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10441 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    10501 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10561 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10621 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10681 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    10741 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10801 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    10861 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    10921 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    10981 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    11041 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    11101 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    11161 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    11221 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11281 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    11341 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    11401 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    11461 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    11521 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
    11581 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    11641 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca
    11701 cgagac
//
