LOCUS       sequence-207674-       11109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a EGFP-tagged full length human 53BP1 
            (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Tiwari A, Addis Jones O, Chan KL
  TITLE     53BP1 can limit sister-chromatid rupture and rearrangements driven 
            by a distinct ultrafine DNA bridging-breakage process.
  JOURNAL   Nat Commun. 2018 Feb 14;9(1):677. doi: 10.1038/s41467-018-03098-y.
  PUBMED    29445165
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-14"; volume: "9(1):677. doi"; pages: " 
            10.1038/s41467-018-03098-y"
COMMENT     Sequence Label: pEGFP-h53BP1 (siRNA resistant)
FEATURES             Location/Qualifiers
     source          1..11109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7479..7496)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7706..7725
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     polyA_signal    7897..8018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7934..7953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7988..8007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8025..8480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8162..8183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8374..8393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8507..8611
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8613..8970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8613..8804
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8636..8656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8821..8956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9005..9799
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9005..9799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9059..9078)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9669..9688
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9987..10006)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10031..10078
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10407..10995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10896..10915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt actcagatct cgagatggac cctactggaa gtcagttgga ttcagatttc
     1381 tctcagcaag atactccttg cctgataatt gaagattctc agcctgaaag ccaggttcta
     1441 gaggatgatt ctggttctca cttcagtatg ctatctcgac accttcctaa tctccagacg
     1501 cacaaagaaa atcctgtgtt ggatgttgtg tccaatcctg aacaaacagc tggagaagag
     1561 cggggcgacg gtaatagtgg gttcaatgaa catttgaaag aaaacaaggt tgcagaccct
     1621 gtggattctt ctaacttgga cacatgtggt tccatcagtc aggtcattga gcagttacct
     1681 cagccaaaca ggacaagcag tgttctggga atgtcagtgg aatctgctcc tgctgtggag
     1741 gaagagaagg gagaagagtt ggaacagaag gagaaagaga aggaagaaga tacttcaggc
     1801 aatactacac attcccttgg tgctgaagat actgcctcat cacagttggg ttttggggtt
     1861 ctggaactct cccagagcca ggatgttgag gaaaatactg tgccatatga agtggacaaa
     1921 gagcagctac aatcagtaac caccaactct ggttatacca ggctgtctga tgtggatgct
     1981 aatactgcaa ttaagcatga agaacagtcc aacgaagata tccccatagc agaacagtcc
     2041 agcaaggaca tccctgtgac agcacagccc agtaaggatg tacatgttgt aaaagagcaa
     2101 aatccaccac ctgcaaggtc agaggacatg ccttttagcc ccaaagcatc tgttgctgct
     2161 atggaagcaa aagaacagtt gtctgcacaa gaacttatgg aaagtggact gcagattcag
     2221 aagtcaccag agcctgaggt tttgtcaact caggaagact tgtttgacca gagcaataaa
     2281 acagtatctt ctgatggttg ctctactcct tcaagggagg aaggtgggtg ttctttggct
     2341 tccactcctg ccaccactct gcatctcctg cagctctctg gtcagaggtc ccttgttcag
     2401 gacagtcttt ccacgaattc ttcagatctt gttgctcctt ctcctgatgc tttccgatct
     2461 actcctttta tcgttcctag cagtcccaca gagcaagaag ggagacaaga taagccaatg
     2521 gacacgtcag tgttatctga agaaggagga gagccttttc agaagaaact tcaaagtggt
     2581 gaaccagtgg agttagaaaa cccccctctc ctgcctgagt ccactgtatc accacaagcc
     2641 tcaacaccaa tatctcagag cacaccagtc ttccctcctg ggtcacttcc tatcccatcc
     2701 cagcctcagt tttctcatga catttttatt ccttccccaa gtctggaaga acaatcaaat
     2761 gatgggaaga aagatggaga tatgcatagt tcatctttga cagttgagtg ttctaaaact
     2821 tcagagattg aaccaaagaa ttcccctgag gatcttgggc tatctttgac aggggattct
     2881 tgcaagttga tgctttctac aagtgaatat agtcagtccc caaagatgga gagcttgagt
     2941 tctcacagaa ttgatgaaga tggagaaaac acacagattg aggatacgga acccatgtct
     3001 ccagttctca attctaaatt tgttcctgct gaaaatgata gtatcctgat gaatccagca
     3061 caggatggtg aagtacaact gagtcagaat gatgacaaaa caaagggaga tgatacagac
     3121 accagggatg acattagtat tttagccact ggttgcaagg gcagagaaga aacggtagca
     3181 gaagatgttt gtattgatct cacttgtgat tcggggagtc aggcagttcc gtcaccagct
     3241 actcgatctg aggcactttc tagtgtgtta gatcaggagg aagctatgga aattaaagaa
     3301 caccatccag aggaggggtc ttcagggtct gaggtggaag aaatccctga gacaccttgt
     3361 gaaagtcaag gagaggaact caaagaagaa aatatggaga gtgttccgtt gcacctttct
     3421 ctgactgaaa ctcagtccca agggttgtgt cttcaaaagg aaatgccaaa aaaagaatgc
     3481 tcagaagcta tggaagttga aaccagtgtg attagtattg attcccctca aaagttggca
     3541 atacttgacc aagaattgga acataaggaa caggaagctt gggaagaagc tacttcagag
     3601 gactccagtg ttgtcattgt agatgtgaaa gagccatctc ccagagttga tgtttcttgt
     3661 gaacctttgg agggagtgga gaagtgctca gattcccagt catgggagga tattgctcca
     3721 gaaatagaac catgtgctga gaatagatta gacaccaagg aagaaaagag tgtagaatat
     3781 gaaggagatc tgaaatcagg gactgcagaa acagaacctg tagagcaaga ttcttcacag
     3841 ccttccttac ctttagtgag agcagatgat cctttaagac ttgaccagga gttgcagcag
     3901 ccccaaactc aggagaaaac aagtaattca ttaacagaag actcaaaaat ggctaatgca
     3961 aagcagctaa gctcagatgc agaggcccag aagctgggga agccctctgc ccatgcctca
     4021 caaagcttct gtgaaagttc tagtgaaacc ccatttcatt tcactttgcc taaagaaggt
     4081 gatatcatcc caccattgac tggtgcaacc ccacctctta ttgggcacct aaaattggag
     4141 cccaagagac acagtactcc tattggtatt agcaactatc cagaaagcac catagcaacc
     4201 agtgatgtca tgtctgaaag catggtggag acccatgatc ccatacttgg gagtggaaaa
     4261 ggggattctg gggctgcccc agacgtggat gataaattat gtctaagaat gaaactggtt
     4321 agtcctgaga ctgaggcgag tgaagagtct ttgcagttca acctggaaaa gcctgcaact
     4381 ggtgaaagaa aaaatggatc tactgctgtt gctgagtctg ttgccagtcc ccagaagacc
     4441 atgtctgtgt tgagctgtat ctgtgaagcc aggcaagaga atgaggctcg aagtgaggat
     4501 ccccccacca cacccatcag ggggaacttg ctccactttc caagttctca aggagaagag
     4561 gagaaagaaa aattggaggg tgaccataca atcaggcaga gtcaacagcc tatgaagccc
     4621 attagtcctg tcaaggaccc tgtttctcct gcttcccaga agatggtcat acaagggcca
     4681 tccagtcctc aaggagaggc aatggtgaca gatgtgctag aagaccagaa agaaggacgg
     4741 agtactaata aggaaaatcc tagtaaggcc ttgattgaaa ggcccagcca aaataacata
     4801 ggaatccaaa ccatggagtg ttccttgagg gtcccagaaa ctgtttcagc agcaacccag
     4861 actataaaga atgtgtgtga gcaggggacc agtacagtgg accagaactt tggaaagcaa
     4921 gatgccacag ttcagactga gagggggagt ggtgagaaac cagtcagtgc tcctggggat
     4981 gatacagagt cgctccatag ccagggagaa gaagagtttg atatgcctca gcctccacat
     5041 ggccatgtct tacatcgtca catgagaaca atccgggaag tacgcacact tgtcactcgt
     5101 gtcattacag atgtgtatta tgtggatgga acagaagtag aaagaaaagt aactgaggag
     5161 actgaagagc caattgtaga gtgtcaggag tgtgaaactg aagtttcccc ttcacagact
     5221 gggggctcct caggtgacct gggggatatc agctccttct cctccaaggc atccagctta
     5281 caccgcacat caagtgggac aagtctctca gctatgcaca gcagtggaag ctcagggaaa
     5341 ggagccggac cactcagagg gaaaaccagc gggacagaac ccgcagattt tgccttaccc
     5401 agctcccgag gaggcccagg aaaactgagt cctagaaaag gggtcagtca gacagggacg
     5461 ccagtgtgtg aggaggatgg tgatgcaggc cttggcatca gacagggagg gaaggctcca
     5521 gtcacgcctc gtgggcgtgg gcgaaggggc cgcccacctt ctcggaccac tggaaccaga
     5581 gaaacagctg tgcctggccc cttgggcata gaggacattt cacctaactt gtcaccagat
     5641 gataaatcct tcagccgtgt cgtgccccga gtgccagact ccaccagacg aacagatgtg
     5701 ggtgctggtg ctttgcgtcg tagtgactct ccagaaattc ctttccaggc tgctgctggc
     5761 ccttctgatg gcttagatgc ctcctctcca ggaaatagct ttgtagggct ccgtgttgta
     5821 gccaagtggt catccaatgg ctacttttac tctgggaaaa tcacacgaga tgtcggagct
     5881 gggaagtata aattgctctt tgatgatggg tacgaatgtg atgtgttggg caaagacatt
     5941 ctgttatgtg accccatccc gctggacact gaagtgacgg ccctctcgga ggatgagtat
     6001 ttcagtgcag gagtggtgaa aggacatagg aaggagtctg gggaactgta ctacagcatt
     6061 gaaaaagaag gccaaagaaa gtggtataag cgaatggctg tcatcctgtc cttggagcaa
     6121 ggaaacagac tgagagagca gtatgggctt ggcccctatg aagcagtaac acctcttaca
     6181 aaggcagcag atatcagctt agacaatttg gtggaaggga agcggaaacg gcgcagtaac
     6241 gtcagctccc cagccacccc tactgcctcc agtagcagca gcacaacccc tacccgaaag
     6301 atcacagaaa gtcctcgtgc ctccatggga gttctctcag gcaaaagaaa acttatcact
     6361 tctgaagagg aacggtcccc tgccaagcga ggtcgcaagt ctgccacagt aaaacctggt
     6421 gcagtagggg caggagagtt tgtgagcccc tgtgagagtg gagacaacac cggtgaaccc
     6481 tctgccctgg aagagcagag agggcctttg cctctcaaca agaccttgtt tctgggctac
     6541 gcatttctcc ttaccatggc cacaaccagt gacaagttgg ccagccgctc caaactgcca
     6601 gatggtccta caggaagcag tgaagaagag gaggaatttt tggaaattcc tcctttcaac
     6661 aagcagtata cagaatccca gcttcgagca ggagctggct atatccttga agatttcaat
     6721 gaagcccagt gtaacacagc ttaccagtgt cttctaattg cggatcagca ttgtcgaacc
     6781 cggaagtact tcctgtgcct tgccagtggg attccttgtg tgtctcatgt ctgggtccat
     6841 gatagttgcc atgccaacca gctccagaac taccgtaatt atctgttgcc agctgggtac
     6901 agccttgagg agcaaagaat tctggactgg caaccccgtg aaaatccttt ccagaatctg
     6961 aaggtactct tggtatcaga ccaacagcag aacttcctgg agctctggtc tgagatcctc
     7021 atgactggtg gtgcagcctc tgtgaagcag caccattcaa gtgcccataa caaagatatt
     7081 gctttagggg tatttgatgt ggtggtgacg gacccctcat gcccagcctc ggtgctgaag
     7141 tgtgctgaag cattgcagct gcctgtggtg tcacaagagt gggtgatcca gtgcctcatt
     7201 gttggggaga gaattggatt caagcagcat ccaaaatata aacacgatta tgtttctcac
     7261 taagcggccg catcgagaga tccgggagac acaacggttt ccctctagcg ggatcaattc
     7321 cgcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     7381 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7441 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     7501 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     7561 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     7621 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     7681 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     7741 ggatgcccag aaggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     7801 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     7861 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     7921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7981 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     8041 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8101 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8161 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8221 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8281 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8341 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     8401 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8461 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8581 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     8641 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8701 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8761 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8941 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     9001 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     9061 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     9121 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     9181 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     9241 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     9301 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     9361 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     9421 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     9481 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     9541 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     9601 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     9661 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     9721 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     9781 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     9841 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9901 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9961 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
    10021 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
    10081 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
    10141 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
    10201 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
    10261 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10321 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    10561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11041 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    11101 gccatgcat
//
