LOCUS       sequence-207696-       12708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in 
            nematode. Cas9 is optimized by removing all piRNA targeting sites to
            allow germline expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC
  TITLE     The piRNA targeting rules and the resistance to piRNA silencing in 
            endogenous genes.
  JOURNAL   Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840.
            Epub 2018 Feb 1.
  PUBMED    29420292
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aao2840"; journalName: "Science"; date: "2018-02-2-
            2"; volume: "359"; issue: "6375"; pages: "587-592"
COMMENT     Sequence Label: pCFJ150-Cas9(dpiRNA)
FEATURES             Location/Qualifiers
     source          1..12708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3800..3816
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3800..3816
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3836..3856
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3857..4456
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             8780..8800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8822..9145
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(9149..9169)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             11207..11218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc ccagtcacga
     2341 cgtcacagca cgtgtgtttt tgaacacggc gatatgtatc tgtagatcat ccccaatttt
     2401 caacaatgaa tctaaatact gaactttttg tgtaatcttt agtgcagaaa aatgaccaaa
     2461 atcagcgatt ttccgattat acccaaaata tctcagctca atgagcacct ataaaaatgt
     2521 aaaaataaag aaagtagtcg aaccatattt tgtaaaggct aaaaaactga ataaattttt
     2581 atccatccag cgcggcgtta caatccgtgg cccatgtcac gcacggcttt caaagaaaaa
     2641 aaaacagtct gtttgcgcga catcgatctg gcaaaaatcc taaaaacgac tatgcgtggc
     2701 tcactttata ttaatatgaa aaatacaaac ttggtggtgc aggtgctgca gaggccatcg
     2761 atgaagaagt ggaggccttg ttgtcgatct acaaaattgt atggttgttt gagattttcc
     2821 ttgaaacgct ctcaatcttg gatatgatat aaattctgga taagatatga tggatatgat
     2881 ctatgtttga tagagtgcat ttgaaaggat aacggccctg cagacatttt tgaaaaaata
     2941 acaaacattt aattttctca ctcgtttagg ctattcccct atttttgata ttccttcgca
     3001 catatgaaaa ctactttttt tcgaaactgt taactccaga atttataaat ctatagccct
     3061 tacttgatta tttattatca tggttactca cctcatgtct ctttctcttt tcaagaagtc
     3121 ttcgttcata tctgttgtct ctttcctcct tttgaatctc tgctttgctc ttcgccattg
     3181 tttcctgaaa ataatgtaac ttgaattgtg taatatactt ttttaatttg aatttggctt
     3241 gtaacgcgga atcactacgt gcgggatcat ttcttactag aaaacccaga aaatgccata
     3301 tttcacttat ctcggggtca tttctaatta gaaaagctac aaaaaccatt tctaattacg
     3361 gtgactttag aactggtcga aatagctaat tagaaatggg tttttgctaa ttagaggtga
     3421 ctctatgtta tctgagtaag cctcgtttgt atgtaaactc acagtactaa taaatgcaag
     3481 acacccgggt ttgtctagat atgaaataat tgaaatatca attctgacag acaataatgg
     3541 taatcttgat aaggagttcc acgcccagga gaacacgtta gttttcttgt ttttgattgc
     3601 gtgcgttatt ttggagaaaa actcgatttt ttacaaaata attttttgaa aggaacactg
     3661 ttcaataagt tttgtctttt ttctcagttg tgatacggtt ttttattctt ttttgtagtt
     3721 atacagaaga ccgttacgaa acagactgat atcgaaacaa acactgatct tacttgcact
     3781 tataatacga ctcactagtc aggaaacagc tatgaccatg attacgccaa gctatcaact
     3841 ttgtatagaa aagttggcac ctttggtctt ttattgtcaa cttccattgg ttcttccatt
     3901 gtttctgtta aattaatgaa tttttcataa aataaagaca ttatacaata taaaaatgaa
     3961 gaatttattg aaaataaact gccagagaga aaaagtatgc aacactcccg ccgagagtgt
     4021 ttgaaatggt gtacggtaca ttttcgtgct aggagttaga tgtgcaggca gcaacgagag
     4081 ggggagagat ttttttgggc cttgtgaaat taacgtgagt tttctggtca tctgactaat
     4141 catgttggtt ttttgttggt ttattttgtt tttatctttg tttttatcca gattaggaaa
     4201 tttaaatttt atgaatttat aatgaggtca aacattcagt cccagcgttt ttcctgttct
     4261 cactgtttag tcgaattttt attttaggct ttcaacaaat gttctaactg tcttatttgt
     4321 gacctcactt tttatatttt tttaattttt aaaaatatta gaagtttcta ggataatttt
     4381 ttcgactttt attctctcta ccgtccgcac tcttcttact tttaaattaa attgtttttt
     4441 tttcagttgg gaaacaccct gcacacccct ttgtacacaa atggacaaaa aatacagcat
     4501 cggactcgac atcggtacca actccgtcgg ttgggccgtc atcaccgacg agtacaaggt
     4561 gcgttggcta atttcctgaa tatcttcaat ttactacatt tcaggtccca tccaagaagt
     4621 tcaaggtcct cggtaacaca gaccgtcact caatcaagaa gaacctcatc ggagccctcc
     4681 tcttcgactc cggagagacc gccgaggcca cccgtctcaa gcgtaccgcc cgtcgtcgtt
     4741 acacccgtcg taagaaccgt atctgctacc tccaggagat cttctccaac gagatggcca
     4801 aggtcgacga ctcattcttc caccgcctcg aggagtcctt cctcgtcgag gaggacaaga
     4861 agcacgagcg tcaccctatc ttcggaaaca tcgtcgacga ggtcgcctac cacgagaagt
     4921 acccaaccat ctaccacctc cgtaagaagc tcgtcgactc caccgacaag gccgacctcc
     4981 gtctcatcta cctcgccctc gcccacatga tcaagttccg tggacacttc ctcatcgagg
     5041 gagacctcaa cccagacaac tccgacgtcg acaagctctt catccagctc gtccagacct
     5101 acaaccagct cttcgaggag aaccctatca acgcctccgg agtcgacgcc aaggccatcc
     5161 tctccgcccg tctctccaag tcccgtcgtc tcgagaacct catcgcccag ctcccaggag
     5221 agaagaagaa cggactcttc ggaaacctca tcgccctctc cctcggactc acccctaact
     5281 tcaagtccaa cttcgacctc gccgaggacg ccaagctcca gctctccaaa gacacctacg
     5341 acgacgacct cgacaacctc ctcgcccaga tcggagacca gtacgccgac ctcttcctcg
     5401 ccgccaaggt cagaagaaaa catgtatttt tgctcactgt aaacattttc agaacctctc
     5461 cgacgccatc ctcctctccg acatcctccg tgtcaacacc gagataacca aggccccact
     5521 ctccgcctcc atgatcaagc gttacgacga gcaccaccag gacctcaccc tcctcaaggc
     5581 cctcgtccgt cagcagctcc cagagaagta caaggagatc ttcttcgacc agtccaagaa
     5641 cggatacgcc ggatacatcg acggaggagc ctcccaggag gagttctaca agttcatcaa
     5701 gccaatcctc gaaaagatgg acggaacaga ggagctcctc gtcaagctca accgtgagga
     5761 cctcctccgt aagcagcgta ccttcgacaa cggatccatc ccacaccaga tccacctcgg
     5821 agagctccac gccatcctcc gtcgtcagga ggacttctac ccattcctga aagacaaccg
     5881 cgaaaagatc gaaaagatac tcaccttccg tatcccatac tacgtcggac cactcgcccg
     5941 tggaaactcc cgtttcgcct ggatgacccg taagtccgag gagaccatca ccccatggaa
     6001 cttcgaggag gtcgtcgaca agggagcctc cgcccagtcc ttcatcgagc gtatgaccaa
     6061 cttcgacaag aacctcccta acgagaaggt cctcccaaag cactccctcc tctacgagta
     6121 cttcaccgtc tacaacgagc tcaccaaggt caagtacgtc accgagggaa tgcgtaagcc
     6181 agccttcctc tccggagaac agaagaaggc catcgtcgac ctcctcttca aggtaagttt
     6241 aaacatgatt ttactaacta actaatctga tttaaatttt cagaccaacc gtaaggtgac
     6301 agtcaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgact ccgtcgagat
     6361 ctccggagtc gaggaccgtt tcaacgcctc cctcggaacc taccacgacc tcctcaagat
     6421 catcaaggac aaggacttcc tcgacaacga ggagaacgag gacatcctcg aggacatcgt
     6481 cctcaccctc accctcttcg aggaccgtga gatgatcgag gagcgtctca agacctacgc
     6541 ccacctcttc gacgacaagg tcatgaagca gctcaagcgt cgtcgttaca ccggatgggg
     6601 acgtctctcc cgtaagctca tcaacggaat ccgtgacaag cagtccggaa agaccatcct
     6661 cgacttcctc aagtccgacg gattcgccaa ccgtaacttc atgcagctca tccacgacga
     6721 ctccctcacc ttcaaggagg acatccagaa ggcccaggtc tccggacagg gagactccct
     6781 ccacgagcac atcgccaacc tcgccggatc cccagccatc aagaagggaa tcctccagac
     6841 cgtcaaggtc gtcgacgagc tcgtcaaggt catgggacgt cacaagccag agaacatcgt
     6901 catcgagatg gcccgcgaga accagaccac ccagaaggga cagaagaact cccgtgagcg
     6961 tatgaagcgt atcgaggagg gaatcaagga gctcggatcc cagatcctca aggagcaccc
     7021 agtcgagaac acccagctcc agaacgagaa gctctacctc tactacctcc agaacggacg
     7081 tgacatgtac gtcgaccagg agctggacat caaccgtctc tccgactacg acgtcgacca
     7141 catcgtccca cagtccttcc tcaaggacga ctccatagac aacaaggtgc tcacccgttc
     7201 cgacaagaac cgtggaaagt ccgacaacgt cccatccgag gaggtcgtca agaagatgaa
     7261 gaactactgg cgtcagctcc tcaacgccaa gctcatcacc cagcgtaagt tcgacaacct
     7321 gaccaaggcc gagcgtggag gactctccga gctcgacaag gccggattca tcaagcgtca
     7381 gctcgtcgag acccgtcaga tcaccaagca cgtcgcccag atcctcgact cccgtatgaa
     7441 caccaagtac gacgagaacg acaagctcat ccgtgaggtc aaggtcatca ccctcaagtc
     7501 caagctagtc tccgacttcc gtaaagactt ccagttctac aaggtccgtg agatcaacaa
     7561 ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc ggaaccgccc tcatcaagaa
     7621 gtacccaaag ctcgagtccg agttcgtcta cggagactac aaggtctacg acgtccgtaa
     7681 ggtaagaatg aagcaaacaa attatgttaa ttccaatttt tagatgatcg ccaagtccga
     7741 acaggagata ggaaaggcca ccgccaagta cttcttctac tccaacatca tgaacttctt
     7801 caagaccgag ataaccctcg ccaacggaga gatccgtaag cgtccactca tcgagaccaa
     7861 cggagagacc ggagagatcg tctgggacaa gggacgtgac ttcgccaccg tccgtaaggt
     7921 cctctccatg ccacaggtca acatcgtcaa gaagaccgag gtccagaccg gaggattctc
     7981 caaggagtcc atcctcccaa agcgtaactc cgacaagctc atcgcccgta aaaaggactg
     8041 ggaccctaag aagtacggag gattcgactc cccaaccgtc gcctactccg tcctcgtcgt
     8101 cgccaaggtc gagaagggaa agtccaagaa gctcaagtcc gtcaaggagc tcctcggaat
     8161 caccatcatg gagcgttcct ccttcgagaa gaacccaatc gacttcctcg aggccaaggg
     8221 atacaaggag gtaaagaaag acctcatcat caagctccca aagtactccc tcttcgagct
     8281 cgagaacgga cgtaagcgta tgctcgcctc cgccggagag ctccagaagg gaaacgagct
     8341 cgccctccca tccaagtacg tcaacttcct ctacctcgcc tcccactacg agaagctcaa
     8401 aggttcccca gaagacaacg agcagaagca gctcttcgtc gagcagcaca agcactacct
     8461 cgacgagatc atcgagcaga tctccgagtt ctccaagcgt gtcatcctcg ccgacgccaa
     8521 cctcgacaag gtcctctccg cctacaacaa gcaccgtgac aagccaatcc gtgagcaggc
     8581 cgagaacatc atccacctct tcaccctcac caacctcgga gccccagccg ccttcaagta
     8641 cttcgacacc accatcgacc gtaagcgtta cacctccacc aaggaggtcc tcgacgccac
     8701 cctcatccac cagtccatca ccggactcta cgagacccgt atcgacctct cccagctcgg
     8761 aggagactcc cgtgccgacc caaagaagaa gcgtaaggtc taattgtaca aagtgggatc
     8821 catgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     8881 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     8941 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     9001 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     9061 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     9121 ttattgtgcc gccaagaaaa aagtctcaca actttattat acatagttga taattcccta
     9181 ggtgagctct ggtaccctct agtcaaggcc ttaagtgagt cgtattacgt agcccgggcc
     9241 tagttctaga cattctctaa tgaaaaaatc tttcagttga aattgaaaat gagttaaagt
     9301 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga
     9361 cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga
     9421 gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag
     9481 ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat
     9541 catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt
     9601 cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt
     9661 tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat
     9721 agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac
     9781 acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga
     9841 tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag
     9901 caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt
     9961 tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact
    10021 tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt
    10081 tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg
    10141 tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg
    10201 gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga
    10261 aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca
    10321 cctgcgacgg gaaggtcgcc gagccgggtg gaatcgatcg ttgttgctcg gctttcatat
    10381 cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt
    10441 gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa
    10501 aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat
    10561 gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt
    10621 tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctattg
    10681 cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg
    10741 aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc
    10801 gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac
    10861 agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga
    10921 agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca
    10981 gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt
    11041 atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag
    11101 ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca
    11161 tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc
    11221 taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca
    11281 aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa tactttttac
    11341 aaggacttgg ataaattggc tcaaagacga agtgagtttg ctaccatcat ggaatcagga
    11401 ggacatttcg acaatgtcat ataatgtttg aaaaatgatt ttaacaaggg tctcattcga
    11461 gataagcaat gctctgagca agtggctcgt cgtcttcaag aatctcttgt tgatattcat
    11521 ttggatcctg ttttgcacga agcatgtgcc atggatattc agagtttttg caaaaaagtt
    11581 ccgccaggtc aaacaatctt tttaattttt atatgatact tcgggtgtat ttttcttctt
    11641 tttcctgttc attttcgctt ttttctcact tttttgtaac aaaatctctc acttttctgt
    11701 aagtcttaac aaaagttcaa ttggctcgat caagtaaaat ttcggccgta tgaacgtttt
    11761 caaaggtttt catttttttt taagttcatt cacaatatca tgaatctctc gtcctttggt
    11821 ttccggtaag tagcgaaata atataagcat acttataaat gaaggaattg aatatagaat
    11881 aaggaaagca taactaccaa taacggaata tagaggtaat actgtaaatg tactgataac
    11941 caccattaca gtattcattg aatatgccac agattgagca atactacgat gtttttgagg
    12001 taccagttca gaggatataa accacgaaat tggaccaact ccagttctga aatttcatta
    12061 ttcgattaaa tcaaaatata tttgaaaaac ctcacccata agtataaccg tagatgatca
    12121 atgcaactag gcatccgtat ttcatcgggt caatcagaat acgtattttt gcgaagaata
    12181 cgaatgcaat tagagctaaa ttattgagaa atgtgaagag aaggagaagc ttgcgtcgac
    12241 caactctgta aacttattca tacaattttg tcttcaaatt ttgaacgttt aaaactatct
    12301 tattcaatga gttagcgggt ttttatagtg agttagttca cgattatcta ataaaactat
    12361 gtccaaacat cctaactgcg accgtattct cactaactcg tcttaaaaac gttgagatta
    12421 ttttaaaccc ttcttcagta caactacagt actgatgcac aaatacaata ataataatac
    12481 attcagacat actctgaaca atgtaacttg tttttgttat actgtggtaa catttattga
    12541 aatgaaatat aaccttactt ttcaatcaca attccaccag taattgttcc agaaacataa
    12601 gctaaaccca ttgcagttgt actccattca gcaatttctg actcaacatt tgcattttct
    12661 agaaagaaag tagaggataa aagaagagcc cataatccga cagggccc
//
