LOCUS       sequence-207838-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA with neomycin 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             246..426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    473..598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1091..1324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1509..1553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1737..1854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1923..2134
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5975..6031
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6050..6844
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6104..6123)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6714..6733
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6860..7448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6913..6933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7331..7342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7520..7753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7831..7952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7868..7887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7922..7941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7992..8127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8147..8166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8148..8166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8176..8193)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8176..8192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8185..8207)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8334..8789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8421..8440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8631..8652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8815..8919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8920..8988,8989..9780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9138..9157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9951..10539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10440..10459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10693..10710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10827..10848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10863..10868,10869..10886,10887..10893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10901..10917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10906..10928
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10925..10941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10925..10941
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10960..10980
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10962..10980
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
       61 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
      121 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
      181 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
      241 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      421 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      481 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      541 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      601 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      661 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      721 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      781 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      841 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      901 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
      961 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1021 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1081 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1141 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1201 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1261 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1321 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1381 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1441 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1501 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1561 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1621 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1681 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccaga ccacaatttt
     1741 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1801 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1861 tattacaggg acagcagaga tccactttgg cgccggctcg agtggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccaccc
     2161 ggaggtttca ctgcacaaca agatggcggc ggcggcggcg agcggagctg gcggggctgc
     2221 cggggccggg actgggggag ccgggcccgc gggccgcctg ctgcctccgc ccgcgccggg
     2281 gtccccagcc gcccccgctg ccgtgtcccc tgcggccggc cagccgcgtc ccccagcccc
     2341 ggcctcccgc ggacccatgc ccgcccgtat cggctactac gagatcgacc gcaccatcgg
     2401 caagggcaac ttcgcggtgg tcaagcgggc cacgcacctc gtcaccaagg ccaaggttgc
     2461 tatcaagatc atagataaga cccagctgga tgaagaaaac ttgaagaaga ttttccggga
     2521 agttcaaatt atgaagatgc tttgccaccc ccatatcatc aggctctacc aggttatgga
     2581 gacagaacgg atgatttatc tggtgacaga atatgctagt ggaggggaaa tatttgacca
     2641 cctggtggcc catggtagaa tggcagaaaa ggaggcacgt cggaagttca aacagatcgt
     2701 cacagctgtc tatttttgtc actgtcggaa cattgttcat cgtgatttaa aagctgaaaa
     2761 tttacttctg gatgccaatc tgaatatcaa aatagcagat tttggtttca gtaacctctt
     2821 cactcctggg cagctgctga agacctggtg tggcagccct ccctatgctg cacctgaact
     2881 ctttgaagga aaagaatatg atgggcccaa agtggacatc tggagccttg gagttgtcct
     2941 ctacgtgctt gtgtgcggtg ccctgccatt tgatggaagc acactgcaga atctgcgggc
     3001 ccgcgtgctg agtggaaagt tccgcatccc attttttatg tccacagaat gtgagcattt
     3061 gatccgccat atgttggtgt tagatcccaa taagcgcctc tccatggagc agatctgcaa
     3121 gcacaagtgg atgaagctag gggacgccga tcccaacttt gacaggttaa tagctgaatg
     3181 ccaacaacta aaggaagaaa gacaggtgga ccccctgaat gaggatgtcc tcttggccat
     3241 ggaggacatg ggactggaca aagaacagac actgcaggcg gagcaggcag gtactgctat
     3301 gaacatcagc gttccccagg tgcagctgat caacccagag aaccaaattg tggagccgga
     3361 tgggacactg aatttggaca gtgatgaggg tgaagagcct tcccctgaag cattggtgcg
     3421 ctatttgtca atgaggaggc acacagtggg tgtggctgac ccacgcacgg aagttatgga
     3481 agatctgcag aagctcctac ctggctttcc tggagtcaac ccccaggctc cattcctgca
     3541 ggtggcccct aatgtgaact tcatgcacaa cctgttgcct atgcaaaact tgcaaccaac
     3601 cgggcaactt gagtacaagg agcagtctct cctacagccg cccacgctac agctgttgaa
     3661 tggaatgggc ccccttggcc ggagggcatc agatggagga gccaacatcc aactgcatgc
     3721 ccagcagctg ctgaagcgcc cacggggacc ctctccgctt gtcaccatga caccagcagt
     3781 gccagcagtt acccctgtgg acgaggagag ctcagacggg gagccagacc aggaagctgt
     3841 gcagaggtac ttggcaaata ggtccaaaag acatacactg gccatgacca accctacagc
     3901 tgagatccca ccggacctac aacggcagct aggacagcag cctttccgtt cccgggtctg
     3961 gcctcctcac ctggtacctg atcagcatcg ctctacctac aaggactcca acactctgca
     4021 cctccctacg gagcgtttct cccctgtgcg ccggttctca gatggggctg cgagcatcca
     4081 ggccttcaaa gctcacctgg aaaaaatggg caacaacagc agcatcaaac agctgcagca
     4141 ggagtgtgag cagctgcaga agatgtacgg ggggcagatt gatgaaagaa ccctggagaa
     4201 gacccagcag cagcatatgt tataccagca ggagcagcac catcaaattc tccagcaaca
     4261 aattcaagac tctatctgtc ctcctcagcc atctccacct cttcaggctg catgtgaaaa
     4321 tcagccagcc ctccttaccc atcagctcca gaggttaagg attcagcctt caagcccacc
     4381 ccccaaccac cccaacaacc atctcttcag gcagcccagt aatagtcctc cccccatgag
     4441 cagtgccatg atccagcctc acggggctgc atcttcttcc cagtttcaag gcttaccttc
     4501 ccgcagtgca atctttcagc agcaacctga gaactgttcc tctcctccca acgtggcact
     4561 aacctgcttg ggtatgcagc agcctgctca gtcacagcag gtcaccatcc aagtccaaga
     4621 gcctgttgac atgctcagca acatgccagg cacagctgca ggctccagtg ggcgcggcat
     4681 ctccatcagc cccagtgctg gtcagatgca gatgcagcac cgtaccaacc tgatggccac
     4741 cctcagctat gggcaccgtc ccttgtccaa gcagctgagt gctgacagtg cagaggctca
     4801 cagtgcacat cagcagccgc cacactatac cacgtcggca ctacagcagg ccctgctgtc
     4861 tcccacgccg ccagactata caagacacca gcaggtaccc cacatccttc aaggactgct
     4921 ttctccccgg cattcgctca ccggccactc ggacatccgg ctgcccccaa cagagtttgc
     4981 acagctcatt aaaaggcagc agcaacaacg gcagcagcag cagcaacagc agcaacagca
     5041 agaataccag gaactgttca ggcacatgaa ccaaggggat gcggggagtc tggctcccag
     5101 ccttggggga cagagcatga cagagcgcca ggctttatct tatcaaaatg ctgactctta
     5161 tcaccatcac accagccccc agcatctgct acaaatcagg gcacaagaat gtgtctcaca
     5221 ggcttcctca cccaccccgc cccacgggta tgctcaccag ccggcactga tgcattcaga
     5281 gagcatggag gaggactgct cgtgtgaggg ggccaaggat ggcttccaag acagtaagag
     5341 ttcaagtaca ttgaccaaag gttgccatga cagccctctg ctcttgagta ccggtggacc
     5401 tggggaccct gaatctttgc taggaactgt gagtcatgcc caagaattgg ggatacatcc
     5461 ctatggtcat cagccaactg ctgcattcag taaaaataag gtgcccagca gagagcctgt
     5521 catagggaac tgcatggata gaagttctcc aggacaagca gtggagctgc cggatcacaa
     5581 tgggctcggg tacccagcac gcccctccgt ccatgagcac cacaggcccc gggccctcca
     5641 gagacaccac acgatccaga acagcgacga tgcttatgta cagctggata acttgccagg
     5701 aatgagtctc gtggctggga aagcacttag ctctgcccgg atgtcggatg cagttctcag
     5761 tcagtcttcg ctcatgggca gccagcagtt tcaggatggg gaaaatgagg aatgtggggc
     5821 aagcctggga ggtcatgagc acccagacct gagtgatggc agccagcatt taaactcctc
     5881 ttgctatcca tctacgtgta ttacagacat tctgctcagc tacaagcacc ccgaagtctc
     5941 cttcagcatg gagcaggcag gcgtgggcag cggcgccacc aacttcagcc tgctgaagca
     6001 ggccggcgac gtggaggaga accccggccc catgaccgag tacaagccca tgattgaaca
     6061 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6121 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6181 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6241 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6301 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6361 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6421 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6481 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6541 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6601 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6661 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6721 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6781 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6841 ctgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6901 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6961 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7021 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7081 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7141 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7201 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7261 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7321 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7381 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7441 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7561 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7741 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     7801 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7981 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8041 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8101 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8161 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8221 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8281 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8761 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8821 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8881 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8941 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9001 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9061 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9121 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9181 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9241 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9301 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9361 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9421 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9481 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9541 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9601 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9661 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9721 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9781 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10621 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10681 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    10741 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    10801 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    10861 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    10921 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
    10981 gaacaaaag
//
