LOCUS       sequence-207839-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant 
            silent mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_CR
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1726..1843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1912..2123
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5964..6020
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6039..6833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6093..6112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6703..6722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6849..7437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6902..6922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7320..7331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7509..7742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7820..7941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7857..7876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7911..7930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7981..8116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8136..8155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8137..8155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8165..8182)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8165..8181)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8174..8196)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8323..8778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8410..8429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8620..8641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8804..8908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8909..8977,8978..9769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9127..9146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9940..10528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10429..10448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10682..10699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10816..10837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10852..10857,10858..10875,10876..10882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10890..10906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10895..10917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10914..10930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10914..10930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10949..10969
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10951..10969
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccagac cacaatttta aaagaaaagg
     1741 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1801 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1861 cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag tgggcagagc
     1921 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     1981 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2041 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2101 acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgccacccg gaggtttcac
     2161 tgcacaacaa gatggcggcg gcggcggcga gcggagctgg cggggctgcc ggggccggga
     2221 ctgggggagc cgggcccgcg ggccgcctgc tgcctccgcc cgcgccgggg tccccagccg
     2281 cccccgctgc cgtgtcccct gcggccggcc agccgcgtcc cccagccccg gcctcccgcg
     2341 gacccatgcc cgcccgtatc ggctactacg agatcgaccg caccatcggc aagggcaact
     2401 tcgcggtggt caagcgggcc acgcacctcg tcaccaaggc caaggttgct atcaagatca
     2461 tagataagac ccagctggat gaagaaaact tgaagaagat tttccgggaa gttcaaatta
     2521 tgaagatgct ttgccacccc catatcatca ggctctacca ggttatggag acagaacgga
     2581 tgatttatct ggtgacagaa tatgctagtg gaggggaaat atttgaccac ctggtggccc
     2641 atggtagaat ggcagaaaag gaggcacgtc gcaagttcaa acagatcgtc acagctgtct
     2701 atttttgtca ctgtcggaac attgttcatc gtgatttaaa agctgaaaat ttacttctgg
     2761 atgccaatct gaatatcaaa atagcagatt ttggtttcag taacctcttc actcctgggc
     2821 agctgctgaa gacctggtgt ggcagccctc cctatgctgc acctgaactc tttgaaggaa
     2881 aagaatatga tgggcccaaa gtggacatct ggagccttgg agttgtcctc tacgtgcttg
     2941 tgtgcggtgc cctgccattt gatggaagca cactgcagaa tctgcgggcc cgcgtgctga
     3001 gtggaaagtt ccgcatccca ttttttatgt ccacagaatg tgagcatttg atccgccata
     3061 tgttggtgtt agatcccaat aagcgcctct ccatggagca gatctgcaag cacaagtgga
     3121 tgaagctagg ggacgccgat cccaactttg acaggttaat agctgaatgc caacaactaa
     3181 aggaagaaag acaggtggac cccctgaatg aggatgtcct cttggccatg gaggacatgg
     3241 gactggacaa agaacagaca ctgcaggcgg agcaggcagg tactgctatg aacatcagcg
     3301 ttccccaggt gcagctgatc aacccagaga accaaattgt ggagccggat gggacactga
     3361 atttggacag tgatgagggt gaagagcctt cccctgaagc attggtgcgc tatttgtcaa
     3421 tgaggaggca cacagtgggt gtggctgacc cacgcacgga agttatggaa gatctgcaga
     3481 agctcctacc tggctttcct ggagtcaacc cccaggctcc attcctgcag gtggccccta
     3541 atgtgaactt catgcacaac ctgttgccta tgcaaaactt gcaaccaacc gggcaacttg
     3601 agtacaagga gcagtctctc ctacagccgc ccacgctaca gctgttgaat ggaatgggcc
     3661 cccttggccg gagggcatca gatggaggag ccaacatcca actgcatgcc cagcagctgc
     3721 tgaagcgccc acggggaccc tctccgcttg tcaccatgac accagcagtg ccagcagtta
     3781 cccctgtgga cgaggagagc tcagacgggg agccagacca ggaagctgtg cagaggtact
     3841 tggcaaatag gtccaaaaga catacactgg ccatgaccaa ccctacagct gagatcccac
     3901 cggacctaca acggcagcta ggacagcagc ctttccgttc ccgggtctgg cctcctcacc
     3961 tggtacctga tcagcatcgc tctacctaca aggactccaa cactctgcac ctccctacgg
     4021 agcgtttctc ccctgtgcgc cggttctcag atggggctgc gagcatccag gccttcaaag
     4081 ctcacctgga aaaaatgggc aacaacagca gcatcaaaca gctgcagcag gagtgtgagc
     4141 agctgcagaa gatgtacggg gggcagattg atgaaagaac cctggagaag acccagcagc
     4201 agcatatgtt ataccagcag gagcagcacc atcaaattct ccagcaacaa attcaagact
     4261 ctatctgtcc tcctcagcca tctccacctc ttcaggctgc atgtgaaaat cagccagccc
     4321 tccttaccca tcagctccag aggttaagga ttcagccttc aagcccaccc cccaaccacc
     4381 ccaacaacca tctcttcagg cagcccagta atagtcctcc ccccatgagc agtgccatga
     4441 tccagcctca cggggctgca tcttcttccc agtttcaagg cttaccttcc cgcagtgcaa
     4501 tctttcagca gcaacctgag aactgttcct ctcctcccaa cgtggcacta acctgcttgg
     4561 gtatgcagca gcctgctcag tcacagcagg tcaccatcca agtccaagag cctgttgaca
     4621 tgctcagcaa catgccaggc acagctgcag gctccagtgg gcgcggcatc tccatcagcc
     4681 ccagtgctgg tcagatgcag atgcagcacc gtaccaacct gatggccacc ctcagctatg
     4741 ggcaccgtcc cttgtccaag cagctgagtg ctgacagtgc agaggctcac agtgcacatc
     4801 agcagccgcc acactatacc acgtcggcac tacagcaggc cctgctgtct cccacgccgc
     4861 cagactatac aagacaccag caggtacccc acatccttca aggactgctt tctccccggc
     4921 attcgctcac cggccactcg gacatccggc tgcccccaac agagtttgca cagctcatta
     4981 aaaggcagca gcaacaacgg cagcagcagc agcaacagca gcaacagcaa gaataccagg
     5041 aactgttcag gcacatgaac caaggggatg cggggagtct ggctcccagc cttgggggac
     5101 agagcatgac agagcgccag gctttatctt atcaaaatgc tgactcttat caccatcaca
     5161 ccagccccca gcatctgcta caaatcaggg cacaagaatg tgtctcacag gcttcctcac
     5221 ccaccccgcc ccacgggtat gctcaccagc cggcactgat gcattcagag agcatggagg
     5281 aggactgctc gtgtgagggg gccaaggatg gcttccaaga cagtaagagt tcaagtacat
     5341 tgaccaaagg ttgccatgac agccctctgc tcttgagtac cggtggacct ggggaccctg
     5401 aatctttgct aggaactgtg agtcatgccc aagaattggg gatacatccc tatggtcatc
     5461 agccaactgc tgcattcagt aaaaataagg tgcccagcag agagcctgtc atagggaact
     5521 gcatggatag aagttctcca ggacaagcag tggagctgcc ggatcacaat gggctcgggt
     5581 acccagcacg cccctccgtc catgagcacc acaggccccg ggccctccag agacaccaca
     5641 cgatccagaa cagcgacgat gcttatgtac agctggataa cttgccagga atgagtctcg
     5701 tggctgggaa agcacttagc tctgcccgga tgtcggatgc agttctcagt cagtcttcgc
     5761 tcatgggcag ccagcagttt caggatgggg aaaatgagga atgtggggca agcctgggag
     5821 gtcatgagca cccagacctg agtgatggca gccagcattt aaactcctct tgctatccat
     5881 ctacgtgtat tacagacatt ctgctcagct acaagcaccc cgaagtctcc ttcagcatgg
     5941 agcaggcagg cgtgggcagc ggcgccacca acttcagcct gctgaagcag gccggcgacg
     6001 tggaggagaa ccccggcccc atgaccgagt acaagcccat gattgaacaa gatggattgc
     6061 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6181 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6241 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6301 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6361 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6421 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6481 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6541 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6601 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6661 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6721 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6781 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtt
     6841 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6901 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6961 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7021 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7081 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7141 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7201 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7261 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7321 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7381 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     7441 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7501 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7561 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7741 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7801 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7921 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8041 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8101 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     8161 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8221 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8281 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     8341 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8401 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8461 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8521 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8581 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8641 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8701 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8761 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8821 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8881 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8941 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9001 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9061 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9121 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9181 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9241 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9301 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9361 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9421 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9481 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9541 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9601 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9661 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9721 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9781 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10621 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10681 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10741 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10801 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10861 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10921 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    10981 ggagctgca
//
