LOCUS       sequence-208149-        1858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of tTA under the Syn1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Sakaguchi R, Leiwe MN, Imai T
  TITLE     Bright multicolor labeling of neuronal circuits with fluorescent 
            proteins and chemical tags.
  JOURNAL   eLife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350.
  PUBMED    30454553
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-11-20"; volume: "7. pii: 40350. doi"; pages: " 
            10.7554/eLife.40350"
COMMENT     Sequence Label: pAAV-Syn1-tTA
FEATURES             Location/Qualifiers
     source          1..1858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   2..131
                     /label=AAV2 ITR
     promoter        213..660
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(675..1292,1293..1298,1299..1334,1335..1337,1338..1373,
                     1374..1376,1377..1412,1413..1421)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1445..1566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1456..1475)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1510..1529
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   1728..1857
                     /label=AAV2 ITR
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagatc tctgcagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg
      241 aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc
      301 cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga
      361 ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc
      421 gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa
      481 actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac
      541 cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac
      601 tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag
      661 tcgaattcgc caccatgtct agactggaca agagcaaagt cataaactct gctctggaat
      721 tactcaatga agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
      781 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg
      841 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
      901 aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg
      961 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
     1021 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
     1081 ccgtgggcca ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag
     1141 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
     1201 tgttcgacca tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
     1261 gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg
     1321 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
     1381 acgctcttga cgattttgac cttgacatgc tccccgggta agcggccgct tcgagcagac
     1441 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1501 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1561 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     1621 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga
     1681 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     1741 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     1801 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagc
//
