LOCUS       sequence-208263-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of proISG15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, 
            Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander
            D
  TITLE     Irreversible inactivation of ISG15 by a viral leader protease 
            enables alternative infection detection strategies.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 
            10.1073/pnas.1710617115. Epub 2018 Feb 20.
  PUBMED    29463763
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1710617115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-6- 6"; volume: "115"; issue: "10"; pages: "2371-2376"
COMMENT     Sequence Label: ISG15 C-term. Domain deltaC intein/chitin bind...
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             329..922
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             953..1105
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQL"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg gctagctcgc gagtcgacgg cggccgcgaa
       61 ttcctcgagg gctcttcctg cggaagagca accacctatg gatgaaccgc tgagcattct
      121 ggtgcgtaat aacaaaggtc gtagcagcac ctatgaagtt cgtctgaccc agacagttgc
      181 acatctgaaa cagcaggtta gcggtctgga aggtgttcag gatgacctgt tttggctgac
      241 ctttgaaggt aaaccgctgg aagatcagct gccgctgggt gaatatggtc tgaaaccgct
      301 gtcaaccgtt tttatgaatc tgcgtctgtg catcacggga gatgcactag ttgccctacc
      361 cgagggcgag tcggtacgca tcgccgacat cgtgccgggt gcgcggccca acagtgacaa
      421 cgccatcgac ctgaaagtcc ttgaccggca tggcaatccc gtgctcgccg accggctgtt
      481 ccactccggc gagcatccgg tgtacacggt gcgtacggtc gaaggtctgc gtgtgacggg
      541 caccgcgaac cacccgttgt tgtgtttggt cgacgtcgcc ggggtgccga ccctgctgtg
      601 gaagctgatc gacgaaatca agccgggcga ttacgcggtg attcaacgca gcgcattcag
      661 cgtcgactgt gcaggttttg cccgcgggaa acccgaattt gcgcccacaa cctacacagt
      721 cggcgtccct ggactggtgc gtttcttgga agcacaccac cgagacccgg acgcccaagc
      781 tatcgccgac gagctgaccg acgggcggtt ctactacgcg aaagtcgcca gtgtcaccga
      841 cgccggcgtg cagccggtgt atagccttcg tgtcgacacg gcagaccacg cgtttatcac
      901 gaacgggttc gtcagccacg ctactggcct caccggtctg aactcaggcc tcacgacaaa
      961 tcctggtgta tccgcttggc aggtcaacac agcttatact gcgggacaat tggtcacata
     1021 taacggcaag acgtataaat gtttgcagcc ccacacctcc ttggcaggat gggaaccatc
     1081 caacgttcct gccttgtggc agcttcatga ctgcnnggaa ggggatccgg ctgctaacaa
     1141 agcccgaaag gaagctgaat tggctgctgc caccgctgaa caataactag cataacccct
     1201 tggggcctct aaacggggtc ttggagggtt ttttgctgaa aggaggaa
//
